Retinol detection kit and preparation method thereof
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A retinol and reagent technology, applied in the field of detection kits for detecting human retinol-binding protein, can solve the problems of being unsuitable for routine testing, cumbersome operation, and long time consumption
Inactive Publication Date: 2012-12-26
谢兵
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Problems solved by technology
These methods have the disadvantages of cumbersome operation, long time consumption, excessive waste of resources, and are not suitable for routine inspection.
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Embodiment 1
[0033] The kit of the present invention is exemplified as a double reagent, wherein:
[0034] Reagent R1:
[0035] Tris buffer 200mmol / L
[0036] Polyethylene glycol 8 mmol / L
[0037] Sodium azide 5 mmol / L
[0038] Sodium edetate 5mmol / L
[0039] Bovine serum albumin 5mmol / L;
[0040] Reagent R2:
[0041] Tris buffer 200mmol / L
[0042] Latex particles conjugated to retinol binding protein monoclonal antibody 5% (v / v)
[0043] Tween-20 5mmol / L
[0044] Sodium azide 3 mmol / L
[0045] Sodium edetate 5mmol / L
[0046] Bovine serum albumin 5mmol / L.
Embodiment 2
[0047] Embodiment 2: Kit use method
[0048] 1. Reagent preparation, the reagent is a liquid double reagent, ready to use after opening the bottle, of which:
[0049] Reagent R1:
[0050] Tris buffer 200mmol / L
[0051] Polyethylene glycol 8 mmol / L
[0052] Sodium azide 5 mmol / L
[0053] Sodium edetate 5mmol / L
[0054] Bovine serum albumin 5mmol / L;
[0055] Reagent R2:
[0056] Tris buffer 200mmol / L
[0057] Latex particles conjugated to retinol binding protein monoclonal antibody 5% (v / v)
[0064] (b) Detection wavelength: the main wavelength is 340nm;
[0065] (c) Reaction time: 10 minutes, wherein, the incubation time is 5 minutes, and the reaction time is 5 minutes.
[0066] 3. Detection steps (all completed in the automatic biochemi...
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Abstract
The invention relates to a detection kit for a retinol binding protein. The interior of a detection kit body comprises reagents R1 and reagents R2, wherein the reagents R1 comprise a buffer solution, polyethylene glycol, sodium azide, sodium ethylene diamine tetracetate and bovine serum albumin; and the reagents R2 comprise a buffer solution, latex grains combined with retinol binding protein monoclonal antibodies, tween-20, sodium azide, sodium ethylene diamine tetracetate and bovine serum albumin. A sample and the reagents are mixed at a certain volume ratio and generate a series of reactions, and then a reactant is placed under a semi / full-automatic biochemical analyzer, and the speed of absorbance change in the position of a main wavelength of 340nm is detected, so that the concentration magnitude of the retinol binding protein is measured and calculated. The detection kit has the advantages of accuracy, stability and convenience.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a detection kit for detecting human retinol binding protein. Background technique [0002] Retinol-binding protein is a binding protein that specifically binds vitamin A in plasma and plays an important role in the metabolism of vitamin A. [0003] The clinical detection of retinol binding protein is helpful for the early diagnosis of hepatitis, diabetes and kidney disease. [0004] Retinol-binding protein is mainly synthesized by the rough endoplasmic reticulum of the liver. Widely distributed in human serum, urine, cerebrospinal fluid. Retinol-binding protein exists as a complex in the blood. When liver cells are damaged. Synthesis of retinol-binding protein is inhibited. Since the half-life of retinol-binding protein is 3 to 12 hours. Its relative molecular weight is small, and it is related to serum total bilirubin, albumin, and prothrombin time. So it can sensitiv...
Claims
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