7 results about "Fatty acid-binding protein" patented technology

The application belongs to the technical field of biological detection, and discloses a cardiovascular marker control article and a preparation method thereof. The cardiovascular marker control article comprises a serum matrix, antigens, a preservative and a stabilizer. The antigens include cardiac troponin I antigens, creatine kinase isoenzyme antigens, myoglobin antigens, amino-terminal brain natriuretic peptide precursor antigens, brain natriuretic peptide antigens, D-dimer antigens, heart-type fatty acid-binding protein antigens, myeloperoxidase antigens and lipoprotein phospholipase A2 antigens. The stabilizer includes at least one of D-trehalose, sucrose, glycine, inositol and polyethylene glycol. The cardiovascular marker control article can cover various cardiovascular disease markers, including heart failure, pulmonary embolism, vasculitis and atherosclerosis, myocardial injury, myocardial infarction and the like. The control article can be used as a third-party control article for indoor and inter-laboratory quality control of a cardiovascular disease screening and auxiliary diagnosis measurement system.

This specification discloses FABP inhibitor compounds and their use in pharmaceutical compositions for treating diseases that highly express any of these FABPs, including cancers, particularly triple-negative breast cancer (TNBC), and other pro-inflammatory diseases, including cardiovascular diseases, obesity or obesity-related disorders, diabetes, dyslipidemia, impaired glucose tolerance or impaired fasting blood glucose, vitiligo, psoriasis, autoimmune disorders, pain, and dementia. This specification also discloses methods for preparing the disclosed compounds.

This invention relates to the application of fatty acid-binding protein 4 (FABP4) inhibitors in the treatment of heart failure with preserved ejection fraction. Specifically, the application involves the oral administration of the fatty acid-binding protein 4 (FABP4) inhibitor BMS309403 for the prevention and / or treatment of heart failure with preserved ejection fraction.

Disclosed herein are FABP inhibitor compounds and their use in pharmaceutical compositions for the treatment of diseases, including cancers that highly express any of FABP, particularly triple negative breast cancer (TNBC), and other inflammation-inducing diseases, including cancer that highly express any of FABP. Comprise cardiovascular diseases, obesity or obesity-related diseases, diabetes, dyslipidemia, impaired blood glucose tolerance or impaired fasting blood glucose, vitiligo, psoriasis, autoimmune diseases, pain and dementia. Also disclosed herein are methods for preparing the disclosed compounds.

The invention provides a preparation method of an intestinal fatty acid binding protein IFABP antigen, which comprises the following steps: S1, designing a mature peptide into a signal peptide-6 * His-linker peptide-IFABP-linker peptide-IFABP-YINK-containing signal peptide dimer protein based on a gene sequence of an NCBI (National Center of Biotechnology Information) login number NP000125.2 encoding human IFABP mature peptide, and performing codon optimization on an amino acid sequence of the signal peptide-6 * His-linker peptide-IFABP-linker peptide-IFABP-YINK to obtain a nucleic acid sequence; s2, cloning the optimized gene into a eukaryotic expression vector, and constructing a recombinant expression plasmid; and S3, transfecting the recombinant plasmid into a mammalian cell 293F, carrying out cell culture after transfection, and purifying to obtain the IFABP antigen. According to the preparation method, the high-purity and high-activity IFABP recombinant antigen can be efficiently obtained on a large scale, and then the high-performance anti-IFABP monoclonal antibody is obtained.

The invention discloses a yang-warming and kidney-tonifying paste patch for preventing diabetes from being converted into diabetic kidney and a preparation method thereof. The traditional Chinese medicine ointment patch is prepared from traditional Chinese medicines such as radix rehmanniae preparata, fructus evodiae, clove, cortex cinnamomi, radix codonopsis, radix puerariae and semen cuscutae, and is prepared by grinding the traditional Chinese medicines into powder, blending the powder into paste and preparing the paste into a cylindrical medicine cake. The traditional Chinese medicine composition acts on the Shenshu acupoint through acupoint application, and traditional Chinese medicine ingredients are used for regulating kidney qi and blood and protecting kidney functions. Clinical tests show that a test group using the application is remarkably superior to a control group in the aspect of improving the traditional Chinese medicine syndromes. The fatty acid binding protein 1 in the early kidney injury marker urine has the effects of regulating lipid metabolism and resisting oxidative stress, can protect renal tubule cells, and has the early kidney injury warning capability, and a test group applying the application has a remarkable effect of reducing the level of the fatty acid binding protein 1. Safety analysis shows that the application is low in adverse reaction occurrence rate and good in safety. The invention provides a traditional Chinese medicine non-drug therapy which has no gastrointestinal burden and is convenient for long-term persistence for preventing and treating the diabetic kidney disease.

A kind of heart-shaped fatty acid binding protein determination kit, including box, box cover, fixed seat, movable plate, sleeve, pull plate, mounting bracket, lock sleeve, the box hinge box cover, box cover connects lock tongue, box connects lock seat, lock tongue is adapted to lock seat, lock tongue connects lock seat, the inside connection fixed seat of box, fixed seat has slide, slide is adapted to movable plate, movable plate connects slide, movable plate slides in slide, movable plate has multiple top rods, fixed seat has multiple placement grooves, placement groove is adapted to top rod, top rod connects placement groove, top rod upper portion connects rubber top block, rubber top block connects reagent bottle, reagent bottle connects placement groove.