L-FABP (Liver-Fatty Acid Binding Protein) rapid detection reagent and preparation method thereof

A detection reagent and rapid technology, which is applied in the field of L-FABP rapid detection reagent and its preparation, can solve the problems of complex reagents, large differences in NC membranes, and uneven release, and achieve the effects of improving accuracy, rapid detection, and high sensitivity

Inactive Publication Date: 2015-09-02
SHANGHAI SUNFORY BIOPHARM INC
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  • Abstract
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AI Technical Summary

Problems solved by technology

The reaction specificity of latex turbidimetry is not good, and the reagents required are relatively complicated
The immunochromatography method is easy to operate, but on the one hand, its sensitivity is not high; on

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A preparation method of L-FABP rapid detection reagent comprises the following steps:

[0042] 1. Antibody labeled with fluorescent microspheres

[0043] Label L-FABP polyclonal antibody and goat anti-mouse IgG on the surface of fluorescent microspheres according to the following procedures:

[0044] Take 50mM MEST (pH 5.0), dilute the antibody to a concentration of 2mg / ml, and the total volume is 100ul;

[0045] Take 100ul of 0.02g / 100ml fluorescent microspheres, add them to the antibody solution, and sonicate for 1min at 90% power (ultrasonic instrument with a total power of 350W, 10-90% power adjustable, ultrasonic frequency 25KHZ, the same below);

[0046] Stir and incubate at room temperature (25°C) for 15 minutes;

[0047] Add 5ul of 0.5g / ml EDC, mix well, and sonicate at 90% power for 1min;

[0048] Add 0.4ul of 1M sodium hydroxide, adjust the pH to 6.5 ± 0.2, and stir the reaction at room temperature for 3 hours;

[0049] Stop the reaction, sonicate with 90...

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Abstract

The invention discloses an L-FABP rapid detection reagent. The L-FABP rapid detection reagent comprises a substrate and a cover plate covering the substrate, wherein a sample feeding region, a reaction region, a detection region and a waste liquid region are sequentially machined on the substrate; each of the sample feeding region, the reaction region, the detection region and the waste liquid region is composed of a micro-fluid channel; the sample feeding region is used for storing a polyester film for filtering a biological sample; a dry fluorescent microsphere marked antibody covers the reaction region; the dry fluorescent microsphere marked antibody comprises a fluorescent microsphere marked L-FABP polyclonal antibody and a fluorescent microsphere marked goat-anti-mouse immune globulin (IgG) antibody; a detection line and a control line cover the detection region; the L-FABP polyclonal antibody covers the detection line; the mouse IgG antibody covers the control line; and the waste liquid region is composed of the micro-fluid channel with a capillary action. The L-FABP rapid detection reagent disclosed by the invention has the advantages of convenience in operation, rapidness in detection, high sensitivity, good accuracy and the like; and a reagent production process is simple and convenient, pollution to the environment is not caused in the production process and the cost is low.

Description

technical field [0001] The invention belongs to the field of medical reagents, in particular to a rapid detection reagent for L-FABP (liver fatty acid binding protein) and a preparation method thereof. Background technique [0002] Acute kidney injury (AKI) is one of the most common emergencies in clinical departments. From mildly elevated serum creatinine to severe anuric AKI, the degree and clinical manifestations of AKI vary. AKI is closely related to the mortality of patients, the occurrence of chronic kidney disease (CKD) and the rate of maintenance dialysis. The incidence of AKI has been increasing year by year, from 610 cases (per million people) in 1988 to 2888 cases (per million people) in 2009. National statistics for the past 10 years show that the proportion of AKI patients in hospitalized patients during the same period is 0.28% to 3.19%, and as high as 30% to 50% in the ICU; the fatality rate is 14.5% to 41.9%. The incidence of AKI in critically ill patients,...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/347
Inventor 李锐闵洪中刘辉荣
Owner SHANGHAI SUNFORY BIOPHARM INC
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