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Kit for detecting heart-type fatty acid binding proteins by latex enhanced immune turbidimetry method

A fatty acid combination and immunoturbidimetric technology, which is applied in the field of medical testing, can solve the problems of unsatisfactory detection sensitivity and specificity, cumbersome operation, waste of resources, etc., and achieve the effect of fast and sensitive determination, good stability, and easy operation

Active Publication Date: 2016-08-31
HONGKUI BIOLOGICAL CHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of cumbersome operation, long time-consuming, excessive waste of resources, and unsatisfactory detection sensitivity and specificity in the detection of heart-type fatty acid binding protein in the prior art, the application provides a detection method that is convenient, easy to operate, and takes a short time. , high sensitivity, good stability, and strong specificity heart-type fatty acid binding protein detection kit

Method used

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  • Kit for detecting heart-type fatty acid binding proteins by latex enhanced immune turbidimetry method
  • Kit for detecting heart-type fatty acid binding proteins by latex enhanced immune turbidimetry method
  • Kit for detecting heart-type fatty acid binding proteins by latex enhanced immune turbidimetry method

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Embodiment 1

[0031] A kit for detecting heart-type fatty acid binding protein by latex-enhanced immunoturbidimetric method, comprising reagent R1 and reagent R2, wherein reagent R1 contains the following components:

[0032] Tris buffer 120mmol / L,

[0033] Polyethylene glycol p-isooctyl phenyl ether 20mmol / L,

[0034] pH is 7.0-8.0,

[0035] Reagent R2 contains the following components:

[0036] Mouse anti-heart fatty acid binding protein monoclonal antibody latex particles 4.5g / L,

[0037]

[0038]

[0039] The pH value is 7.0-8.0,

[0040] In the latex particles of the mouse anti-human heart fatty acid binding protein monoclonal antibody latex particles, the latex particles with a diameter of 10-30nm account for 10% of the total volume, the latex particles with a diameter of 30-60nm account for 60% of the total volume, and the latex particles with a diameter of 60-100nm account for 10% of the total volume. Latex particles make up 30% of the total volume,

[0041] The volume ra...

Embodiment 2

[0045] A kit for detecting heart-type fatty acid binding protein by latex-enhanced immunoturbidimetric method, comprising reagent R1 and reagent R2, wherein reagent R1 contains the following components:

[0046] Tris buffer 120mmol / L,

[0047] Polyethylene glycol p-isooctyl phenyl ether 20mmol / L,

[0048] pH is 7.0-8.0,

[0049] Reagent R2 contains the following components:

[0050] Mouse anti-heart fatty acid binding protein monoclonal antibody latex particles 4.5g / L,

[0051]

[0052] The pH value is 7.0-8.0,

[0053]In the latex particles of the mouse anti-human heart fatty acid binding protein monoclonal antibody latex particles, the latex particles with a diameter of 10-30nm account for 30% of the total volume, the latex particles with a diameter of 30-60nm account for 40% of the total volume, and the latex particles with a diameter of 60-100nm account for 30% of the total volume. Latex particles make up 30% of the total volume,

[0054] The volume ratio of reagen...

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Abstract

The invention relates to the technical field of medical detection, in particular to a kit for detecting heart-type fatty acid binding proteins by a latex enhanced immune turbidimetry method. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains a Tris buffer solution and polyethylene glycol p-isooctyl phenyl ether, and has the pH of 7.0 to 8.0; the reagent R2 contains mouse-anti-human heart-type fatty acid binding protein monoclonal antibody latex particles, a Tris buffer solution, albumin bovine serum, alkyl glycoside APG0810, mannitol and polyethylene glycol p-isooctyl phenyl ether, and has the pH of 7.0 to 8.0. According to the kit disclosed by the invention, the ratios of the latex particles with different particle sizes are strictly defined, so that the sensitivity and the specificity of detection are improved; therefore, a surfactant and stabilizing agent combination suitable for the system is obtained; on the premise of high measurement speed and sensitivity and high specificity, the whole system is high in stability and easy and convenient to operate, and is suitable for being matched with a clinically full-automatic or semi-automatic biochemical analyzer in use.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a heart-shaped fatty acid binding protein detection kit, and also to a preparation method of the heart-shaped fatty acid binding protein detection kit. Background technique [0002] Heart fatty acid binding protein (hFABP) is a novel small cytosolic protein abundant in the heart. It is highly cardiac specific (ie, predominantly expressed in cardiac tissue), but is also expressed at low concentrations in tissues other than the heart. After the occurrence of myocardial ischemic injury, hFABP can be found in the blood as early as 1-3 hours after the onset of chest pain, peak at 6-8 hours and the level returns to normal within 24-30 hours. Cardiac fatty acid binding cytoplasmic protein consists of 132 amino acids with a molecular weight of 15 kDa. [0003] The normal range of hFABP in serum depends on the test and method. In 1991, Tanaka et al. reported a normal range of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 王保全尚凯王霞
Owner HONGKUI BIOLOGICAL CHINA CO LTD
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