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Paper-based analysis device and method based on zinc oxide nanowire fluorescence enhancement

A zinc oxide nanowire, fluorescence enhancement technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of relying on testing centers and hospitals, and the detection of AMI is expensive, achieving good selectivity, short detection time, and lightening the environment. effect of stress

Inactive Publication Date: 2019-12-13
千藤(南京)环保科技有限公司
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Problems solved by technology

[0005] The technical problems to be solved by the present invention include: first, the paper-based analysis device developed is cheap, which solves the problem of high cost of detecting AMI; Centers and hospitals; third, the developed paper-based analysis method can detect three myocardial markers at the same time, improving the accuracy of AMI diagnosis

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  • Paper-based analysis device and method based on zinc oxide nanowire fluorescence enhancement
  • Paper-based analysis device and method based on zinc oxide nanowire fluorescence enhancement
  • Paper-based analysis device and method based on zinc oxide nanowire fluorescence enhancement

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[0035] The present invention will be further described below in conjunction with the accompanying drawings. The following examples are only used to illustrate the technical solution of the present invention more clearly, but not to limit the protection scope of the present invention.

[0036] The present invention adopts the following technical scheme: a paper-based analysis device based on zinc oxide nanowire fluorescence enhancement, which is characterized in that it includes a detection layer A and a sample addition layer B, and the detection layer A includes three detection areas, which are respectively used to detect fatty acids Binding protein (FABP), cardiac troponin I (cTnI) and myoglobin (myoglobin), samples are added to the central region of the sample addition layer B, and then the sample addition layer B is folded onto the detection layer In A, the sample is shunted to the three detection areas to realize the simultaneous detection of the FABP, cTnI and myoglobin. ...

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Abstract

The invention discloses a paper-based analysis device and a paper-based analysis method based on zinc oxide nanowire fluorescence enhancement. The device comprises a detection layer A and a sample adding layer B. The detection layer A comprises three detection areas which are used for detecting fatty acid binding protein FABP, cardiac troponin cTnI and myoglobin respectively. A sample is added tothe central area of the sample adding layer B, and then, the sample adding layer B is folded onto the detection layer A so that the sample is distributed to the three detection areas to realize simultaneous detection of fatty acid binding protein FABP, cardiac troponin cTnI and myoglobin. The paper-based analysis device and the paper-based analysis method disclosed by the invention can detect thethree myocardial markers at the same time and have high sensitivity and good selectivity for the detection of FABP, cTnI and myoglobin, and the detection limits of the three myocardial markers are 1.36ng / mL, 1.00ng / mL and 2.38ng / mL respectively.

Description

technical field [0001] The invention relates to a paper-based analysis device and method based on zinc oxide nanowire fluorescence enhancement, in particular to a method of integrating zinc oxide nanowires on a paper base and simultaneously detecting a variety of myocardial A paper-based analysis device and method for markers belong to the technical field of paper-based analysis and detection. Background technique [0002] Since 2007, the Whitesides research group of Harvard University started the research on paper-based analysis and detection, and used photolithography to prepare a paper-based analysis device for the detection of glucose and protein, which promoted the application of paper-based analysis technology in medicine. They also superimposed multi-layer planar paper chips, and fixed them with double-sided adhesive to make the first three-dimensional paper-based analysis device, which realized high-throughput detection of various substances, and proposed a microflui...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/533
CPCG01N33/6887G01N33/6803G01N33/54306G01N33/54346G01N33/533
Inventor 于海东郭雪英许智惠
Owner 千藤(南京)环保科技有限公司
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