Detection kit for ischemic modified albumin and preparation method thereof

A detection kit and a technology for albumin, which are applied to the detection kit for ischemia-modified albumin and its preparation field, can solve the problems of poor stability and bottle opening stability, poor anti-interference performance of reagents, poor sample correlation, etc. The effect of increasing anti-interference ability, improving sample correlation, and increasing stability

Active Publication Date: 2018-06-08
SHANGHAI KEHUA BIO ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since DTT itself is a strong reducing agent, it is easily oxidized by air and has a relatively short half-life, so the reagent has disadvantages such as poor long-term use stability and bottle opening stability, poor anti-interference performance of the reagent, and poor sample correlation.

Method used

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  • Detection kit for ischemic modified albumin and preparation method thereof
  • Detection kit for ischemic modified albumin and preparation method thereof
  • Detection kit for ischemic modified albumin and preparation method thereof

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Embodiment Construction

[0042] In order to make the object, technical solution and advantages of the present invention clearer, various embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. However, those of ordinary skill in the art can understand that, in each implementation manner of the present invention, many technical details are provided for readers to better understand the present application. However, even without these technical details and various changes and modifications based on the following implementation modes, the technical solution claimed in this application can also be realized.

[0043] The first embodiment of the present invention relates to a detection kit for ischemic modified albumin, comprising R1 reagent and R2 reagent, wherein,

[0044] The components and concentrations of the R1 reagent are:

[0045]

[0046] The components and concentrations of the R2 reagent are:

[0047]

[0048] It should be note...

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Abstract

The invention relates to the field of biotechnologies, and discloses a detection kit for ischemia modified albumin and a preparation method of the detection kit. The kit comprises a reagent R1and a reagent R2, wherein stabilizing agents of the R2 comprise ethanolamine, a nonionic surfactant and 2,2'-diethylamino dithio dihydrochloride, and the massic volume of ethanolamine is 20%-80%. According to the preparation method, by adding ethanolamine, the nonionic surfactant and 2,2'-diethylamino dithio dihydrochloride into the reagent R2, dithiothreitol (DTT) in the reagent R2 of the kit can not be easily oxidized and is long in half-life period, therefore, the detection kit for the ischemia modified albumin is high in stability, good in sample correlation and high in anti-jamming capability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit for ischemic modified albumin and a preparation method thereof. Background technique [0002] Ischemia modified albumin (IMA) is when tissue ischemia / reperfusion occurs, due to the destruction of the amino acid sequence of serum albumin by free radicals, resulting in albumin and transition metals (including Cu, Co and Ni ), the albumin whose binding ability to transition metals changes due to ischemia is called ischemia-modified albumin (IMA). [0003] In recent years, markers of myocardial injury have developed rapidly. The detection of the quality of ECG (electrocardiogram), troponin (cTn), myoglobin (Myo), creatine kinase isoenzyme MB (CK-MB) has been widely used in clinical practice, gradually replacing the original enzyme activity detection. However, the diagnosis of acute coronary syndrome (ACS) has not been properly resolved. [0004] ACS is characterized b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/32
Inventor 王会李基韩立敏杨君赵亚茹
Owner SHANGHAI KEHUA BIO ENG
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