Kit for detecting ischemia modified albumin and method for making same

A kit and albumin technology, applied in biological testing, material testing products, etc., can solve the problems of poor stability of reagents, easy decomposition, slow reaction time, etc., achieve good clinical application prospects, improve sensitivity, and ensure stability. sexual effect

Inactive Publication Date: 2009-06-17
SHANGHAI FOSUN PHARMA (GROUP) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reaction time is also relatively slow, and sometimes the reaction is difficult to carry out on the automatic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0067] formula

[0068] Reagent 1

[0069] Sodium citrate 300mmol / L

[0070] Lactic acid 50mmol / L

[0071] C O Cl 2 40mmol / L

[0072] Triton x-100 5ml / L

[0073] NaCl 40mmol / L

[0074] MnCl 2 30mmol / L

[0075] Deionized water 1L

[0076] Reagent 2

[0077] Na 2 HPO 4 50mmol / L

[0078] NaH 2 PO 4 20mmol / L

[0079] KCl 80mmol / L

[0080] DTT 6g / L

[0081] Glutathione 8g / L

[0082] proclin300 0.5ml / L

[0083] Deionized water 1L

[0084] method:

[0085] (1) Add 0.8L of deionized water to container one;

[0086] (2) Add 300mmol sodium citrate, 50mmol lactic acid, 10mmol C O Cl 2 , 10ml of Triton x-100, 20mmol NaCl and 20mmol MnCl 2 ;

[0087] (3) Add 0.8L of deionized water into container two;

[0088] (4) Add 10mmol Na 2 HPO 4 , 10mmolNaH 2 PO 4 , 20mmol KCl, 15gDTT, 10g glutathione and 0.3mlproclin300;

[0089] (5) adjust the pH value of the mixed solution in the contai...

example 2

[0094] Reagent 1

[0095] Glycine 100mmol / L

[0096] Hydrochloric acid 50mmol / L

[0097] HgCl2 50mmol / L

[0098] C O Cl 2 50mmol / L

[0099] Triton x-100 2ml / L

[0100] NaCl 40mmol / L

[0101] MnCl 2 30mmol / L

[0102] Deionized water 1L

[0103] Reagent 2

[0104] Na 2 HPO 4 50mmol / L

[0105] NaH 2 PO 4 50mmol / L

[0106] KCl 80mmol / L

[0107] DTT 10g / L

[0108] Glutathione 8g / L

[0109] proclin300 0.5ml / L

[0110] Deionized water 1L.

[0111] Preparation method is the same as example 1.

example 3

[0113] Reagent 1

[0114] Phthalic acid 100mmol / L

[0115] Hydrochloric acid 50mmol / L

[0116] C O Cl 2 50mmol / L

[0117] Triton x-100 10ml / L

[0118] NaCl 30mmol / L

[0119] MnCl 2 50mmol / L

[0120] Deionized water 1L

[0121] Reagent 2

[0122] Na 2 HPO 4 60mmol / L

[0123] NaH 2 PO 4 40mmol / L

[0124] KCl 40mmol / L

[0125] DTT 10g / L

[0126] Glutathione 10g / L

[0127] proclin300 0.5ml / L

[0128] Deionized water 1L

[0129] Preparation method is the same as example 1.

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PUM

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Abstract

The invention discloses a kit for detecting ischemia modified albumin. The kit consists of a reagent 1 and a reagent 2. A surfactant is added to accelerate the reaction of the ischemia modified albumin; and a protective agent is selected to ensure the stability of the reagents. The kit has the better clinical application prospect. The invention further provides a preparation method of the kit.

Description

Technical field: [0001] The present invention relates to biological reagents. In particular, it relates to a kit for detecting ischemic modified albumin and a preparation method thereof. Background technique: [0002] Ischemia-modified albumin is when ischemia / reperfusion occurs, due to the destruction of the amino acid sequence of serum albumin by free radicals, etc., resulting in changes in the binding ability of albumin to transition metals, which occurs due to ischemia and transition metals Albumin with altered binding capacity is called ischemia-modified albumin (IMA). [0003] In recent years, markers of myocardial injury have developed rapidly. The detection of the quality of troponin (cTn), myoglobin (Myo), and creatine kinase isoenzyme MB (CK-MB) has been widely used clinically, gradually replacing the original detection of enzyme activity. However, the diagnosis of acute coronary syndrome (ACS) has not been properly resolved. [0004] In addition, ECG (electroc...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 景晟
Owner SHANGHAI FOSUN PHARMA (GROUP) CO LTD
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