Ischemia modified albumin detection reagent and detection method thereof

A detection reagent and detection method technology are applied in the field of ischemia-modified albumin detection to achieve the effects of improving performance, meeting clinical needs, and having good accuracy and stability.

Active Publication Date: 2014-02-05
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of poor reproducibility and specificity and serious interference in the detection of ischemic modified albumin in the above prior art, the present invention provides A high sensitivity, good anti-interference performance, stable ischemia-modified albumin detection reagent

Method used

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  • Ischemia modified albumin detection reagent and detection method thereof
  • Ischemia modified albumin detection reagent and detection method thereof
  • Ischemia modified albumin detection reagent and detection method thereof

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Experimental program
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Embodiment 1

[0036] Detection reagents for ischemia-modified albumin, including reagent R1 and reagent R2:

[0037] 1) The composition of its R1 is:

[0038] PIPES (piperazine-1,4-diethanesulfonic acid) buffer (pH=7.6, 25°C) ·························· 0.1mol / L

[0039] CoCl 2 (Cobalt chloride) ······················································································· 24mmol / L

[0040] Lauryl dimethyl betaine ··········································································· 3g / L

[0041] Preservative NaN 3···························································································· 0.5g / L

[0042] 2) The components of reagent R2 are:

[0043] MES buffer (pH=5.0, 25°C) ································································· 0.1mol / L

[0044] Thiadiazole azodimethylaminophenol (TDAMAP) ················································· 24mmol / L

[0045] Lauryl dimethyl betaine ·······················································...

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Abstract

The invention relates to the technical field of detection of ischemia modified albumin and in particular relates to an ischemia modified albumin detection reagent. A reagent R1 contains a buffer solution, cobalt chloride, dodecyl dimethyl betaine and a preservative; a reagent R2 contains a buffer solution, thiadiazolyazo dimethylaminophenol, dodecyl dimethyl betaine and a preservative. A novel color developing agent, namely the thiadiazolyazo dimethylaminophenol is adopted, so that interference of metal ions such as Pd, Ni, Zn and Cu is effectively avoided, the reagent is stably and easily preserved at low toxicity, and the stability of the reagent is greatly improved. A novel ampholytic surfactant, namely the dodecyl dimethyl betaine is adopted, so that the determination performance is obviously improved, the reagent has obvious effects of maintaining stabilization of the system and avoiding lipid turbidity, and the stability and interference resistance of the reagent are also obviously improved.

Description

technical field [0001] The invention relates to the technical field of detection of ischemia-modified albumin, in particular to a detection reagent of ischemia-modification albumin and a detection method using the detection reagent. Background technique [0002] Ischemia-modified albumin (IMA) is due to the amino acid sequence of serum albumin being damaged by free radicals during ischemia, resulting in changes in the ability to bind transition metals. Ischemia-modified albumin is mainly used for auxiliary diagnosis of acute myocardial ischemia, and is of great significance for excluding acute coronary syndrome (ACS) such as acute myocardial infarction and unstable angina. It is an ideal new type of drug sensitive to myocardial ischemia Mark. Compared with the traditional markers of myocardial injury, the blood concentration of ischemia-modified albumin (IMA) will increase in a short period of time after the onset of ischemia, but at this time the irreversible damage of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31
CPCG01N21/78G01N33/6803G01N33/683G01N2021/775
Inventor 谭柏清李志明甘宜梧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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