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Ischemia modified albumin determination colour-developing agent and preparation method thereof

A chromogenic agent and albumin technology, which is applied in the field of medical testing, can solve the problems of difficulty in storage, the chromogenic agent is easily oxidized by air, and has poor stability, and achieves the effects of rapid detection, convenient preparation and low price.

Active Publication Date: 2012-01-18
ZYBIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compounds commonly used for cobalt ion color development include dithiothreitol (DTT), 1-nitroso-2-naphthol, etc. At present, the color developer prepared with DTT is the main one, and the color developer is easy to Oxidized by air, poor stability, difficult to preserve

Method used

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  • Ischemia modified albumin determination colour-developing agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Weigh 0.8 grams of 1-nitroso-2-naphthol and 1.2 grams of sodium hydroxide, dissolve them in 100 milliliters of purified water, and make solution A; weigh 0.5 grams of citric acid, dissolve them in 100 milliliters of purified water, and prepare into solution B; use solution B to adjust solution A to pH 7.50, and make solution C

[0019] (2) Measure 100 ml of solution C, weigh 0.5 g of fructose, 0.05 ml of Tween 20, and 0.01 ml of Proclin 300 into solution C, stir evenly, and prepare a chromogen for the determination of ischemia-modified albumin.

Embodiment 2

[0021] (1) Weigh 1.0 grams of 1-nitroso-2-naphthol and 1.4 grams of sodium hydroxide, dissolve them in 100 milliliters of purified water, and make solution A; weigh 0.8 grams of citric acid, dissolve them in 100 milliliters of purified water, and prepare into solution B; use solution B to adjust solution A to pH8.00, and make solution C

[0022] (2) Measure 100 ml of solution C, weigh 0.6 g of fructose, 0.08 ml of Tween 20, and 0.02 ml of Proclin 300 into solution C, stir evenly, and prepare a chromogen for the determination of ischemia-modified albumin.

Embodiment 3

[0024] (1) Weigh 1.2 grams of 1-nitroso-2-naphthol and 1.6 grams of sodium hydroxide, dissolve them in 100 milliliters of purified water, and make solution A; weigh 1.0 grams of citric acid, dissolve them in 100 milliliters of purified water, and prepare into solution B; use solution B to adjust solution A to pH8.50, and make solution C

[0025] (2) Measure 100 ml of solution C, weigh 0.8 g of fructose, 0.10 ml of Tween 20, and 0.04 ml of Proclin 300 into solution C, stir evenly, and prepare a chromogen for the determination of ischemia-modified albumin.

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Abstract

The invention discloses an ischemia modified albumin determination colour-developing agent and a preparation method thereof. The preparation method comprises the steps of: taking 0.8-3.6 parts of 1-nitroso-2-naphthol and 1.2-3.5 parts of sodium hydroxide, dissolving in 100ml of purified water, and preparing into a solution A; taking 0.5-4.0 parts of citric acid, dissolving into 100ml of purified water, and preparing into a solution B; and blending the solution A with the solution B until the pH reaches 7.50-10.00, and preparing into a solution C; taking 100ml of solution C, taking 0.5-3.0 parts of fructose, 0.05-0.5 part of Tween 20 and 0.01-0.06 part of Proclin 300, adding into the solution C, uniformly stirring, and preparing into the ischemia modified albumin determination colour-developing agent. The invention has the advantages that the ischemia modified albumin determination colour-developing agent can be used for detecting ischemia modified albumin more sensitively, components of the ischemia modified albumin are common and are lower in price; and the whole preparation is more convenient and the preparation procedure is simple and easy to operate.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to an in vitro diagnostic reagent. Background technique [0002] Ischemia modified albumin (IMA) is a new biochemical marker discovered in recent years, which has unique advantages in the early diagnosis of acute myocardial infarction (AMI). IMA can rise within 5-10 minutes of myocardial ischemia and last for 6 hours. Its sensitivity in diagnosing myocardial ischemia is superior to all current markers and other tests. IMA is usually determined by albumin cobalt binding (albumin cobalt binding, ACB) test, under normal circumstances, the N-terminal of serum albumin (HA) can have a strong interaction with transition metal elements (such as Co, Cu and Ni, etc.) binding ability. Add cobalt chloride solution, cobalt ions combine with the N-terminus of albumin, and the concentration of free cobalt in the solution is low. When myocardial ischemia, IMA does not combine with Co...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
Inventor 吉权李民友潘能科
Owner ZYBIO INC
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