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Method, reagent and kit for quantitatively detecting ischemia modified albumin

A technology for quantitative determination of albumin, applied in the measurement of color/spectral characteristics, material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. It can solve the problem of expensive and unsuitable for routine inspection. , time-consuming and other problems, to achieve the effect of simple operation and fully automatic analysis

Inactive Publication Date: 2015-06-03
ZHEJIANG KAICHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have the disadvantages of cumbersome operation, long time-consuming, expensive, excessive waste of resources, and are not suitable for routine testing, especially large-scale epidemiological examination or simultaneous detection of many items of clinical large-scale specimens

Method used

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  • Method, reagent and kit for quantitatively detecting ischemia modified albumin
  • Method, reagent and kit for quantitatively detecting ischemia modified albumin
  • Method, reagent and kit for quantitatively detecting ischemia modified albumin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Configure the following reagent I and reagent II of the present invention according to the following ingredients and ratios:

[0021] Reagent I:

[0022] Sodium dihydrogen phosphate 80mmol / L

[0023] Disodium hydrogen phosphate 80mmol / L

[0024] Cobalt chloride 12mmol / L

[0025] Reagent II:

[0026] Sodium dihydrogen phosphate 80mmol / L

[0027] Disodium hydrogen phosphate 80mmol / L

[0028] Dithiothreitol 10mmol / L

[0029] Mix 200 μl of reagent I and 30 μl of serum sample in a sample tube, incubate at 37°C for 5 minutes, use Hitachi 7180 automatic biochemical analyzer, measure the absorbance A1 at the main wavelength of 510nm and the secondary wavelength of 700nm, and then add Mix 100 μl of reagent II, incubate at 37°C for 5 minutes, and measure the absorbance A2 at the same wavelength. Calculate the ΔA sample according to the following formula (1). Use the same method and conditions to measure the absorbance value of the standard solution, and calculate the △A s...

Embodiment 2

[0034] Configure the following reagent I and reagent II of the present invention according to the following ingredients and ratios:

[0035] Reagent I:

[0036] Sodium dihydrogen phosphate 30mmol / L

[0037] Disodium hydrogen phosphate 30mmol / L

[0038] Cobalt chloride 5mmol / L

[0039] Reagent II:

[0040] Sodium dihydrogen phosphate 30mmol / L

[0041] Disodium hydrogen phosphate 30mmol / L

[0042] Dithiothreitol 5mmol / L

[0043] Mix 200 μl of reagent I and 30 μl of serum sample in a sample tube, incubate at 37°C for 5 minutes, use Abbott C16000 automatic biochemical analyzer, measure the absorbance A1 at the main wavelength of 510nm and the secondary wavelength of 700nm, and then add to the sample Mix 100 μl of reagent II, incubate at 37°C for 5 minutes, and measure the absorbance A2 at the same wavelength. Calculate the ΔA sample according to the following formula (1). Use the same method and conditions to measure the absorbance value of the standard solution, and calcu...

Embodiment 3

[0047] Configure the following reagent I and reagent II of the present invention according to the following ingredients and ratios:

[0048] Reagent I:

[0049] Sodium dihydrogen phosphate 100mmol / L

[0050] Disodium hydrogen phosphate 100mmol / L

[0051] Cobalt chloride 50mmol / L

[0052] Reagent II:

[0053] Sodium dihydrogen phosphate 100mmol / L

[0054] Disodium hydrogen phosphate 100mmol / L

[0055] Dithiothreitol 30mmol / L

[0056] Mix 200 μl reagent I and 30 μl serum sample in the sample tube, incubate at 37°C for 5 minutes, use the Olympus AU400 automatic biochemical analyzer, measure the absorbance A1 at the main wavelength of 510nm and the secondary wavelength of 700nm, and then Add 100 μl of reagent II to the sample, mix well, incubate at 37°C for 5 minutes, and measure the absorbance A2 at the same wavelength. Calculate the ΔA sample according to the following formula (1). Use the same method and conditions to measure the absorbance value of the standard solutio...

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Abstract

The invention relates to a reagent for quantitatively detecting the content of ischemia modified albumin in human serum. The reagent comprises a reagent I and a reagent II which are respectively placed, wherein the reagent I comprises sodium dihydrogen phosphate, disodium hydrogen phosphate and cobalt chloride, and the reagent II comprises sodium dihydrogen phosphate, disodium hydrogen phosphate and dithiothreitol. The kit and the detection method only need dozens of microlitres of serum without centrifugal or electrophoresis and other separation treatments, is simple and convenient to operate, can be used for meeting the requirement of full-automatic analysis, and are suitable for timely accurate detection of large-scale samples.

Description

technical field [0001] This application relates to a quantitative determination method, reagent and kit for ischemia-modified albumin in human serum Background technique [0002] Ischemia-modified albumin is when ischemia / reperfusion occurs, due to the destruction of the amino acid sequence of serum albumin by free radicals, etc., resulting in changes in the binding ability of albumin to transition metals, which occurs due to ischemia and transition metals Albumin with altered binding capacity. [0003] Ischemia-modified albumin can detect acute myocardial ischemia earlier, is a sensitive indicator for detecting early myocardial ischemia, is also a biomarker for the diagnosis of acute coronary syndrome, and can also indicate the early stage of cerebral hemorrhage and acute coronary syndrome. [0004] Current methods for the determination of ischemia-modified albumin include colorimetric assays, liquid chromatography, mass spectrometry, and nuclear magnetic resonance. These...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
Inventor 梁朝阳
Owner ZHEJIANG KAICHENG BIOTECH
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