Kit for rapidly detecting ischemia modified albumin and application of kit

A kit and albumin technology, which is applied in the field of medical testing, can solve problems such as poor stability, low detection precision, and poor repeatability of measurement results, and achieve good stability, good repeatability, and improved accuracy and stability.

Inactive Publication Date: 2017-05-24
GUANGZHHOU HUAHONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the disadvantages of poor repeatability of measurement results, low detection precision and poor stability of the prior art ischemia-modified albumin kit

Method used

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  • Kit for rapidly detecting ischemia modified albumin and application of kit
  • Kit for rapidly detecting ischemia modified albumin and application of kit
  • Kit for rapidly detecting ischemia modified albumin and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. A kit for rapid detection of ischemia-modified albumin

[0029] The kit for rapid detection of ischemia-modified albumin includes reagent R1 and reagent R2 with a volume ratio of 6:1,

[0030] The reagent R1 is composed of the following components: 90mmol / L of glycine-sodium hydroxide buffer solution with a pH value of 8.5, 50mmol / L of cobalt chloride, 2mol / L of cetyltrimethylammonium chloride and proclin3000.05mol / L.

[0031] The preparation method of the reagent R1 is as follows: add cobalt chloride, cetyltrimethylammonium chloride and proclin300 into the glycine-sodium hydroxide buffer solution with a pH value of 8.5 and stir evenly to obtain the reagent R1.

[0032] The reagent R2 is composed of the following components: 90 mmol / L tris buffer solution with a pH value of 7.5, 20 mmol / L of α-pyridylazo-β-naphthol, 20 mmol / L of hexadecyl trimethyl chloride Ammonium 2mol / L, dipotassium hydrogen phosphate 0.1mol / L, stabilizer 2mmol / L and proclin3000.05mol / L...

Embodiment 2

[0034] Example 2. A kit for rapid detection of ischemia-modified albumin

[0035] The kit for rapid detection of ischemia-modified albumin includes reagent R1 and reagent R2 with a volume ratio of 6:1,

[0036] The reagent R1 is composed of the following components: 120mmol / L of glycine-sodium hydroxide buffer solution with a pH value of 8.8, 70mmol / L of cobalt chloride, 4mol / L of cetyltrimethylammonium chloride and proclin3000.08mol / L.

[0037]The preparation method of reagent R1 is similar to Example 1.

[0038] The reagent R2 is composed of the following components: 120 mmol / L tris buffer solution with a pH value of 8.2, 32 mmol / L of α-pyridylazo-β-naphthol, 32 mmol / L of hexadecyl trimethyl chloride Ammonium 4mol / L, dipotassium hydrogen phosphate 0.2mol / L, stabilizer 3mmol / L and proclin3000.08mol / L; the stabilizer is composed of sodium alginate and sodium oxalate in a weight ratio of 5:2.

[0039] The preparation method of reagent R2 is similar to that of Example 1.

Embodiment 3

[0040] Example 3. A kit for rapid detection of ischemia-modified albumin

[0041] The kit for rapid detection of ischemia-modified albumin includes reagent R1 and reagent R2 with a volume ratio of 6:1,

[0042] The reagent R1 is composed of the following components: 140mmol / L of glycine-sodium hydroxide buffer solution with a pH value of 9.0, 80mmol / L of cobalt chloride, 6mol / L of cetyltrimethylammonium chloride and proclin3000.1mol / L.

[0043] The preparation method of reagent R1 is similar to Example 1.

[0044] The reagent R2 is composed of the following components: 140 mmol / L tris buffer solution with a pH value of 8.5, 40 mmol / L of α-pyridylazo-β-naphthol, 40 mmol / L of cetyl trimethyl chloride Ammonium 6mol / L, dipotassium hydrogen phosphate 0.3mol / L, stabilizer 5mmol / L and proclin3000.1mol / L; the stabilizer is composed of sodium alginate and sodium oxalate in a weight ratio of 6:1.

[0045] The preparation method of reagent R2 is similar to that of Example 1.

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Abstract

The invention belongs to the technical field of medical examination, and in particular relates to a kit for rapidly detecting ischemia modified albumin and application of the kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 mainly consists of a glycine-sodium hydroxide buffer solution, cobalt chloride, hexadecyl trimethyl ammonium chloride and a preservative; and the reagent R2 mainly consists of a trihydroxymethyl aminomethane buffer solution, alpha-pyridine azo-beta-naphthol, hexadecyl trimethyl ammonium chloride, dipotassium phosphate, a stabilizing agent and a preservative. The kit for rapidly detecting ischemia modified albumin, which is provided by the invention, is high in detection result precision, good in repeatability and good in interference resistance, also has the advantages of being good in stability, low in price and easy to preserve, is a relatively ideal ischemia modified albumin detection kit, and is beneficial to clinical application of kits for rapidly detecting ischemia modified albumin.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a kit for rapidly detecting ischemia-modified albumin and an application thereof. Background technique [0002] Human serum albumin (Human serum albumin, HSA) is a protein in human plasma, and its non-glycosylated single-chain polypeptide contains 585 amino acids with a molecular weight of 66kD. Under the action of oxygen free radicals, the amino acid terminal structure of human serum albumin changes, resulting in weakened albumin binding ability to some exogenous metals cobalt, copper, nickel, called ischemia modified albumin (ischemia modified albumin) albumin, IMA). In recent years, many studies have found that ischemia-modified albumin is an ideal biochemical marker of acute myocardial ischemia. Compared with the traditional markers of acute myocardial ischemia, the blood concentration of ischemia-modified albumin (IMA) will increase in a short time afte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 陈立国邹伟权张亚丽李庆祥母润红王涛苏焱
Owner GUANGZHHOU HUAHONG BIOLOGICAL TECH
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