Reagent casing for detecting blood-lacking modification albumin and method thereof

Abstract

It is a detecting ischemia modified albumin (IMA) reagent kit and detection method, and the reagent kit contains different division to remove the background interference material device, which can be centrifugal ultrafiltration IMA detection reagent kit, immunomagnetic beads IMA detection reagent kit, immunochromatography IMA detection reagent kit, immune chromatography membrane IMA detection board reagent kit or immune membrane filtration plate IMA detection reagent kit. After division removal of the background interference material, it can enhance the specific of detection IMA reagent kit and detection method, and using this reagent kit and hospital existing equipments, in about half an hour, it can measure the IMA value to diagnosis myocardial ischemia symptoms. Particularly suitable for bedside rapid detection kit development, ease to use, low cost, it is a very development promising product.

Description

field of invention [0001] The invention relates to a kit for detecting ischemic modified albumin and a detection method thereof. This type of kit uses the albumin cobalt binding test to detect cobalt ions that are not bound to human albumin after removing the background interference of albumin, and the obtained value can be used to diagnose whether the patient has symptoms of myocardial ischemia. technical background [0002] Acute Coronary Syndrome (ACS) is a large category of diseases that cause chest discomfort and other symptoms due to acute myocardial ischemia. It is a common clinical heart and vascular emergency and is also the main cause of acute death. ACS covers a group of progressive diseases, including Unstable Angina (UA), Non-ST Segment Elevation Myocardial Infarction (NSTEMI), ST Segment Elevation Myocardial Infarction (referred to as STEMI) and sudden cardiac death. The pathological basis of ACS is the thrombosis of unstable plaques in the coronary arteries....

AI Technical Summary

Problems solved by technology

Generally speaking, as long as there are parameters on the machine, the biochemical reagent kit should be able to be used on the automatic biochemical analyzers of various brands. The possible reason why the existing technology cannot be used on general biochemical analyzers is that the IMA kit detected by its IMA kit is positive and negative. There is no obvious difference in sexual OD value, the signal is too weak and needs special amplification

Since manual spectrophotometers or small and medium-sized biochemical analyzers cannot effectively amplify the small photoelectric signal differences by tens of thousands of times, they are not suitable for use.

The emergency laboratories of hospitals in my country are generally not equipped with large-scale biochemical analyzers, but ACS are all emergency patients, which has formed an obstacle for the popularization and use of IMA indicators in clinical rapid diagnosis of myocardial ischemia.

[0010] 2. A small change in the OD value of the IMA kinetic curve in the prior art can make the IMA value change in a large range

[0011] According to the IMA kinetic curve of the Bar-Or D method, the OD value on the abscissa only changes within the range of less than 0.3 from the negative low value to the positive high value, while the IMA value on the ordinate changes in a large range of about 200 units, that is to say, the OD A slight change in the IMA value will cause a large change in the IMA value. In this way, the IMA-negative high-value samples can easily become positive, and the IMA-positive low-value samples are likely to be overestimated. This change may be caused by systematic errors and is not It must be caused by the inherent differences in the sample itself, which will cause the overestimation or underestimation of the patient's ischemic condition, which will cause errors in clinical judgment

[0012] 3. The specificity of existing technical kits and detection methods is not high

[0014] In summary, acute coronary syndrome is a dangerous condition, and IMA is an ideal reference index for early ischemia, but the deficiencies in existing technical kits and detection methods limit its further promotion and use, so it is urgent to develop a detection method The IMA kit can improve the specificity of IMA by removing background interference, especially by reducing the OD value of IMA-negative samples; a kinetic curve that does not cause large changes in IMA values ​​due to small changes in OD values ​​is required ; Need a kind of kit that can satisfy common small and medium-sized automatic biochemical analyzer and even common spectrophotometer manual detection IMA; A kind of bedside quick reagent can meet the urgent, simple and correct needs of clinical ACS diagnosis

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