Kit for determination of ischemia modified albumin and preparation method thereof
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A technology of albumin and kits, which is applied in the fields of medicine and biochemistry, can solve the problems of low accuracy and poor stability, and achieve the effects of high accuracy, improved stability, and stable existence
Inactive Publication Date: 2016-11-09
ANHUI IPROCOM BIOTECH CO LTD
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Problems solved by technology
[0008] The technical problem to be solved by the present invention is to provide a kit for measuring ischemia-modified albumin and its preparation method in order to overcome the defects of poor stability and low accuracy in the prior art
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Embodiment 1
[0058] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein
[0059] Reagent R1:
[0060] Tris Buffer 100mmol / L
[0061] Cobalt chloride 0.50 mmol / L
[0062] Sodium azide 5g / L
[0063] Its solvent is purified water.
[0064] Reagent R2:
[0065] Na 2 HPO 4 100mmol / L
[0066] Dithiothreitol 1.5g / L
[0067] Sodium azide 5g / L
[0068] Its solvent is purified water.
Embodiment 2
[0070] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein
[0071] Reagent R1:
[0072] 4-Hydroxyethylpiperazine propanesulfonic acid buffer 200mmol / L
[0073] Cobalt chloride 1.00 mmol / L
[0074] Sodium azide 1g / L
[0075] Its solvent is purified water.
[0076] Reagent R2:
[0077] Na2 HPO 4 10mmol / L
[0078] Dithiothreitol 3.0g / L
[0079] Sodium azide 10g / L
[0080] Its solvent is purified water.
Embodiment 3
[0082] Kit preparation and method of use
[0083] 1. Prepare the reagent according to the content of the following components:
[0084] Reagent R1:
[0085] Tris Buffer 100mmol / L
[0086] Cobalt chloride 0.50 mmol / L
[0087] Sodium azide 5g / L
[0088] Its solvent is purified water.
[0089] Reagent R2:
[0090] Na 2 HPO 4 100mmol / L
[0091] Dithiothreitol 1.5g / L
[0092] Sodium azide 5g / L
[0093] Its solvent is purified water.
[0094] 2. Parameter setting of automatic biochemical analyzer
[0095] (a) Detection temperature: 37°C;
[0096] (b) Detection wavelength: main wavelength 505nm, secondary wavelength 700nm;
[0097] (c) Reaction time: 10 minutes, among them, the incubation time is 5 minutes, measure and read the absorbance A1 immediately after adding the reagent R2, read the absorbance A2 after 5 minutes, and calculate the change of absorbance ΔA = A2-A1;
[0098] (d) Reaction direction: positive reaction.
[0099] 3. Detection steps
[0100] (a) Mix...
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Abstract
The invention discloses a kit for determination of ischemia modified albumin and a preparation method thereof, wherein the kit includes double-liquid components of a reagent R1 and a reagent R2 which are independent with each other and includes the components and the corresponding content: the reagent R1 including 10-200 mmol / L of a buffer liquid, 0.01-1.00 mmol / L of cobalt chloride, 1-10 g / L of a stabilizing agent, and a solvent being purified water; and the reagent R2 including 10-200 mmol / L of Na2HPO4, 0.1-3.0 g / L of a chromogenic agent, 1-10 g / L of a stabilizer, and a solvent being purified water. The preparation method includes the following steps: according to the component content, preparing the reagents; mixing a to-be-tested serum sample with the reagent R1 and the reagent R2, and carrying out full reaction; determining the absorbance difference value after the reaction with a fully automatic biochemical analyzer; and calculating the concentration of the ischemia modified albumin in the sample according to the absorbance change value. The kit has the advantages of high accuracy and the like.
Description
technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring ischemia-modified albumin and a preparation method thereof. Background technique [0002] During myocardial ischemia, when human serum albumin passes through the ischemic site, the amino acid sequence of serum albumin is destroyed by free radicals, etc., and its amino terminal is modified. This kind of albumin whose N-terminal is damaged or occupied by copper is called Ischemia-modified albumin (IMA), which is characterized by a decrease in the binding rate of the N-terminal and transition metal ions such as cobalt, myocardial ischemia 5-10min, ischemia-modified albumin can be detected in the blood, 1-2h Peak, 3-6h to return to the basic level. [0003] Ischemia-modified albumin is a biomarker for the diagnosis of acute coronary syndrome (ACS). ACS has the characteristics of acute onset, rapid changes, uneven clinical manifestations and ris...
Claims
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