A method of promoting keratin dissolution and enhancing strength of a keratin material

A keratin material and keratin technology, applied in the field of materials, can solve the problems of low strength and poor solubility of keratin materials, and achieve the effects of reducing cytotoxicity, enhancing mechanical strength, and improving mechanical strength

Inactive Publication Date: 2017-06-20
SUZHOU BAIRUI BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Aiming at the problem of poor solubility in water of regenerated keratin and low s...

Method used

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  • A method of promoting keratin dissolution and enhancing strength of a keratin material
  • A method of promoting keratin dissolution and enhancing strength of a keratin material
  • A method of promoting keratin dissolution and enhancing strength of a keratin material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 0.3g of keratin powder and add it to 2ml of water or phosphate buffer (pH=7.4), then add cysteine ​​accounting for 1.5% of the protein, stir to dissolve, and let it stand at room temperature to form keratin. protein gel.

Embodiment 2

[0027] Weigh 0.15g of keratin powder and add it to 2ml of water or buffer solution (pH=7.0-12.0), then add dithiothreitol, which accounts for 5% of the protein, stir to dissolve, and let it stand at room temperature. Forms a keratin gel.

Embodiment 3

[0029] Weigh 0.6g of keratin powder and add it to 2ml of water or buffer solution (pH=7.0-12.0), then add glutathione accounting for 1.5% of the protein, stir to dissolve, and pour it into the model. After the solvent evaporates, a keratin film is formed.

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Abstract

The invention relates to a method of promoting keratin dissolution and enhancing strength of a keratin material. The keratin relates to water-insoluble keratin or keratin the solubility of which in water is low, and is characterized in that the disulfide bond content of the keratin is high. The method of promoting dissolution of the water-insoluble keratin includes adding the keratin into an aqueous solution, and adding a certain amount of a reductant at the same time to break disulfide bonds to form free mercapto groups, thus promoting keratin dissolution. The reductant includes cysteine, dithiothreitol, glutathione, mercaptan and sodium pyrosulfite, or keratin or peptides with reducibility, or other compounds capable of inducing disulfide bond breakage and formation of the free mercapto groups, such as proteins or peptides with reducibility. The method is also characterized in that the dissolved keratin comprises the free mercapto groups so that the dissolved keratin can be subjected to disulfide bond crosslinking again under oxidation conditions, thus significantly improving mechanical strength of the keratin material. The keratin material can includes keratin hydrogel, film, sponge, fibers, and a plurality of other forms.

Description

technical field [0001] The invention relates to a method for promoting the dissolution of keratin in water, and a method for preparing materials such as high-strength keratin hydrogel, film, sponge and fiber by using the keratin solution. Background technique [0002] Keratin is an insoluble fibrous protein, a structural protein of ectodermal cells, widely present in animal skin and skin appendages, such as hair, hooves, shells, claws, horns, scales, etc. A large number of studies in recent years have shown that keratin is a high-quality biomedical material with good biocompatibility and not rejected by the body's immune system, and has broad application prospects. Most prominently, the amino acid sequence of keratin derived from wool and other sources was found to contain Arg-Gly-Asp (RGD) tripeptide sequence. This tripeptide sequence is recognized as an effective binding site for cell binding in the extracellular matrix, and has the function of promoting cell adsorption. ...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/075C08J5/18C08J9/28C08L89/00C08K5/37C08K3/30D01F4/00D01F1/10
CPCC08K5/37C08J3/075C08J3/24C08J5/18C08J9/28C08J2201/0484C08J2389/00C08K3/30D01F1/10D01F4/00C08L89/00
Inventor 杨光聂华丽
Owner SUZHOU BAIRUI BIOLOGICAL SCI & TECH CO LTD
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