A liquid-based cell preservation solution

A liquid-based cell and preservation solution technology, which is applied in the field of pathological examination, can solve the problems of unfavorable cell dispersion and observation, easy agglomeration and deposition of protein mucus, and unstable pH value, so as to reduce the loss of cell rupture, facilitate popularization and use, and reduce The effect of agglomeration

Inactive Publication Date: 2011-11-30
XIAOGAN CENT HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing cell preservation solution does not take long to fix the cells and maintain the stability of the cell structure. The cells are easy to deteriorate after more than three days at room temperature, and 50% methanol is added with formaldehyde as a fixed preservative, which is easy to cause cells to form agglomerates and protein Mucus is also easy to agglomerate and deposit, which is not conducive to the dispersion and observation of cells
Neith

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation method of a liquid-based cell preservation solution of the present invention is to weigh and measure the components in the following table;

[0017] Sodium dihydrogen phosphate (Na2HPO4) 42.3mL1mol

[0018] Sodium hydrogen phosphate dihydrate (NaH2PO4) 57.7mL1mol

[0019] 99.5% ethanol 420ml

[0020] Disodium ethylenediaminetetraacetic acid (EDTA) 3g

[0021] Sodium chloride 1g

[0022] Potassium chloride 0.4g

[0023] 37% formaldehyde 54ml

[0024] Dithiothreitol 2g

[0025] Calcium acetate 0.35g

[0026] Magnesium acetate 0.64g

[0027] The preparation steps of a liquid-based cell preservation solution of the present invention are as follows;

[0028] 1 Prepare sodium phosphate buffer solution: mix sodium dihydrogen phosphate (Na2HPO4) and sodium hydrogen phosphate dihydrate (NaH2PO4) storage solutions, add water to dilute to 1000ml, and measure 618ml.

[0029] 2. Add disodium ethylenediaminetetraacetic acid (EDTA) to ethanol, and heat to 50-7...

Embodiment 2

[0034] The preparation method of a liquid-based cell preservation solution of the present invention is to weigh and measure the components in the following table;

[0035] Sodium dihydrogen phosphate (Na2HPO4) 44.3mL1mol

[0036] Sodium hydrogen phosphate dihydrate (NaH2PO4) 52.7mL1mol

[0037] 100% methanol 410ml

[0038] Disodium ethylenediaminetetraacetic acid (EDTA) 2.8g

[0039] Sodium chloride 1g

[0040] Potassium chloride 0.4g

[0041] 37% formaldehyde 52ml

[0042] Dithiothreitol 2.1g

[0043] Calcium acetate 0.35g

[0044] Magnesium acetate 0.64g

[0045] The preparation steps of a liquid-based cell preservation solution of the present invention are as follows;

[0046] 1 Prepare sodium phosphate buffer: mix sodium dihydrogen phosphate (Na2HPO4) and sodium hydrogen phosphate dihydrate (NaH2PO4) storage solution, add water to dilute to 1000ml. Measure 628ml.

[0047] 2. Add disodium ethylenediaminetetraacetic acid (EDTA) to methanol, and heat to 50-70°C to f...

Embodiment 3

[0052] The preparation method of a liquid-based cell preservation solution of the present invention is to weigh and measure the components in the following table;

[0053] Sodium dihydrogen phosphate (Na2HPO4) 40mL1mol

[0054] Sodium hydrogen phosphate dihydrate (NaH2PO4) 58mL1mol

[0055] 99.5% Methanol 440ml

[0056] Disodium ethylenediaminetetraacetic acid (EDTA) 3.2g

[0057] Sodium chloride 1g

[0058] Potassium chloride 0.4g

[0059] 37% formaldehyde 50ml

[0060] Dithiothreitol 2g

[0061] Calcium acetate 0.32g

[0062] Magnesium acetate 0.62g

[0063] The preparation steps of a liquid-based cell preservation solution of the present invention are as follows;

[0064] 1 Prepare sodium phosphate buffer: mix sodium dihydrogen phosphate (Na2HPO4) and sodium hydrogen phosphate dihydrate (NaH2PO4) storage solution, add water to dilute to 1000ml. Measure 600ml.

[0065] 2. Add disodium ethylenediaminetetraacetic acid (EDTA) to methanol, and heat to 50-70°C to fully di...

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Abstract

The invention relates to a pathological examination, in particular to a liquid-based cell preservation solution used in cytopathological examination of human cervical mucus, sputum, urine, pleural fluid, tracheal mucus and the like. It is characterized in that it is prepared from alcohols, sodium phosphate buffer, edetate disodium, sodium chloride 0.08%-0.12%, potassium chloride, formaldehyde, dithiothreitol, calcium acetate, magnesium acetate, etc. As a result, it can not only maintain the stability of the cell structure. It can reduce the agglomeration and precipitation of cell mucus and the loss of cell rupture, and can also make cells easy to stain, improve the clarity of cell preparation, facilitate the smooth progress of pathological examination, and the cost is low, which is conducive to popularization and use.

Description

technical field [0001] The invention relates to a pathological examination, in particular to a liquid-based cell preservation solution used in cytopathological examination of human cervical mucus, sputum, urine, pleural fluid, tracheal mucus and the like. Background technique [0002] The existing cell preservation solution does not take long to fix the cells and maintain the stability of the cell structure. The cells are easy to deteriorate after more than three days at room temperature, and 50% methanol is added with formaldehyde as a fixed preservative, which is easy to cause cells to form agglomerates and protein Mucus is also easy to agglomerate and deposit, which is not conducive to the dispersion and observation of cells. Neither can solve the problem that there are a large number of red blood cells and mucus in the specimen, because these components will directly affect the cell volume after the cells are made on the glass slide, resulting in the loss of the cell vol...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 杨继洲杨娟戴一霏何惠华沈红安崔可舜
Owner XIAOGAN CENT HOSPITAL
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