This invention discloses a low-serum MDCC-MSB1 (MSB1)
suspension culture medium and an inactivated
chicken infectious anemia virus (TIV) vaccine. The culture medium comprises amino acids,
carbon source, additives, vitamins,
inorganic salts, buffer salts, and water, and further contains newborn calf serum (NBCS). Using this medium, low-cost, high-density
suspension culture of MSB1 cells can be achieved, significantly improving
cell culture stability. After 30 consecutive passages at a 1% volume ratio of NBCS, the viability of MSB1 cells remains above 94%, with uniform
cell morphology and a clumping rate of <3%, making it suitable for large-scale
bioreactor production. Furthermore, when applied to the preparation of an inactivated
chicken infectious anemia virus vaccine, it can comprehensively improve
virus propagation efficiency, increasing
virus production efficiency by more than 25%, with a
virus titer of not less than 1.0 × 10⁻⁶. 8.0 TCID 50 / ml, ensuring stable quality in large-scale production.