Swab eluting solution with sample preservation and inactivation functions

Abstract

The invention discloses a swab eluting solution with sample preservation and inactivation functions. The swab eluting solution is prepared from guanidinium isothiocyanate, ethylenediamine tetraacetic acid, a buffering solution, dithiothreitol, a surfactant, glass beads or polypropylene plastic beads and de-ionized water, wherein the contents of the components in the eluting solution are as follows: 20 weight / volume percent to 55 weight / volume percent of the guanidinium isothiocyanate, 1mM to 25mM of the ethylenediamine tetraacetic acid, 20mM to 100mM of the buffering solution, 1 weight / volume percent to 5 weight / volume percent of the dithiothreitol, 1 percent to 5 percent of the surfactant and 3 to 10 glass beads or polypropylene plastic beads. The prepared eluting solution is low-cost and stable in performance; the prepared eluting solution can be used for preserving DNA / RNA (Deoxyribonucleic Acid / Ribonucleic Acid), and elution and normal-temperature preservation and transportation of a swab sample are realized; pathogens can be inactivated, so that the swab eluting solution has a sample inactivation effect, and risks of exposed infection in a laboratory can be greatly reduced; the guanidinium isothiocyanate which is used in the eluting solution is a common chemical component for extracting nucleic acid, so that the subsequent extraction of the nucleic acid is not influenced.

Description

technical field [0001] The present invention relates to a biological sample treatment liquid, in particular to a swab eluate with sample preservation and inactivation functions. Background technique [0002] In order to accurately diagnose diseases, swabs are used to collect specimens (microorganisms, exfoliated cells or secretions, etc.), and the specimens are preserved, cultured, and separated for further processing. It is a common method for clinical medical examinations. [0003] DNA / RNA degradation of nucleic acid samples is a common problem in PCR detection. At present, the common sample processing solutions are generally physiological saline or PBS eluent. During the preservation of nucleic acid samples treated with these eluents, pathogens will rupture and release nucleic acids, but physiological saline and PBS eluents cannot inhibit DNase / RNase activity, nucleic acid is easily degraded, thus affecting the accuracy of detection results. In actual clinical nucleic ...

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Problems solved by technology

However, this preservation solution has no preservation effect on the RNA samples in the sample; at the same time, because the cells are not inactivated, the virus in the cells may be transmitted to the inspection operator during the operation

[0006] In this technical field, Trizol preservation solution is also used. This preservation solution can better preserve RNA samples, and can also inactivate viruses by heating. However, due to the presence of toxic volatile gases, it is harmful to the operator.

[0007] In addition, RNase inhibitor enzymes are used to preserve RNA samples, but the cost is relatively high, and its application is limited

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