Method for extracting total RNA (Ribonucleic Acid) from woody mangrove plants and special extracting solution thereof

Abstract

The invention discloses a method for extracting total RNA (Ribonucleic Acid) from woody mangrove plants and special extracting solution thereof. The extracting solution provided in the invention comprises Tris-HCl, EDTA (Ethylene Diamine Tetraacetic Acid), NaCl, SDS (Sodium Dodecyl Sulfonate), DTT (Dithiothreitol), beta-mercaptoethanol and water, wherein the final concentration of the Tris-HCl is 75-125 mmol / L, the final concentration of the EDTA is 400-600 mmol / L, the final concentration of the NaCl is 400-600 mmol / L, the final concentration of the SDS is 1%-3% (in weight percent), the final concentration of the DTT is 3-8 mmol / L, and the final concentration of the beta-mercaptoethanol is 1%-3% (in weight percent); and the pH value is 8-9. The method provided in the invention is used for extracting the total RNA from the woody mangrove plants by using the extracting solution. The RNA extracted by the method can be directly used for synthesizing cDNA (complementary Desoxyribonucleic Acid) and carrying out molecular biology operations, such as library establishment, molecular clone and the like.

Description

Technical field [0001] The invention relates to a method for extracting total RNA of woody mangrove plants and a special extracting solution thereof. Background technique [0002] Mangrove (Mangrove) is a woody plant community on the tropical coast. It is a tropical and southern subtropical coastal plant community. It is named because it is mainly composed of plants of the mangrove family. Mangroves are characteristic plants of wetlands. There are 61 species worldwide: Kadelia candel (Kadelia candel) and Rhizophora stylosa (Rhizophora stylosa) of the mangrove family, and Lumnitzera racemosa (Lumnitzera racemosa) of the Junzi family. [0003] It is a precious species resource with the unique characteristics of the ecosystem on the edge of the sea and land. With the rapid development of industry and agriculture in modern river basins, the rapid growth of population and economy in coastal cities, and human disturbance, environmental pollution in the estuary bay area has become increa...

AI Technical Summary

Problems solved by technology

Therefore, improper handling of polysaccharides will lead to two consequences in the extraction of RNA, which will affect further biological experiments.

First, a large amount of RNA is also removed while removing polysaccharides, which affects the yield of RNA; second, the polysaccharides in RNA cannot be completely removed, forming a precipitate that is insoluble in water, or a viscous solution after dissolution

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