Neuron-specific enolase stabilizer and preparation method thereof

Abstract

The invention relates to a neuron-specific enolase stabilizer. The stabilizer is prepared from a Tris.HCl buffer solution, NaCl, sucrose, trehalose, casein, bovine serum albumin, PEG6000, lysine, disodium ethylene diamine tetraacetate, dithiothreitol, glycerin, Tween-20, Triton X-100, aprotinin with the final concentration of 100-300 KIU/mL, and Proclin 300, and above substances are uniformly mixed in Millipore ultrapure water used as a solvent, is filtered by a 0.22 [mu]m filter membrane, and finally is preserved at 0-10 DEG C. The stabilizer greatly improves the preservation stability of neuron-specific enolase, prolongs the shelf life of a kit, simplification of the production technology and the doctor's detection operation process of a neuron-specific enolase detection kit, improvement of the metamorphism resistance in the preparation, transportation and storage process of the neuron-specific enolase, and reduction of inter-assay variation caused by a freeze-drying technology.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnostic reagents, in particular to a neuron-specific enolase stabilizer and a preparation method thereof. The stabilizer provided by the invention is used for the preservation of NSE, which can significantly improve the stability of the chemical properties of the NSE, maintain its spatial conformation, and improve its heat resistance. Background technique [0002] Neuron-specific enolase (Neuron specific Enolase, NSE) is an isoenzyme of enolase. The nucleotide sequence of the NSE gene is 2423 bp in length, and the open reading frame is from 226th to 1531 bases, with a length of 1305bp, encoding all 434 amino acids. The biological half-life of NSE may be about 20 hours. NSE has a molecular weight of 78kDa and an isoelectric point of pH4.7. It is an acidic protease that is mainly involved in glycolysis and catalyzes the conversion of 2-phosphoglycerol into enol phosphopyruvate. [0003] NSE is...

AI Technical Summary

Problems solved by technology

[0007] (1) Freeze-drying technology requires the use of freeze-drying machines, which increases equipment investment and maintenance management costs;

[0008] (2) Due to the extreme instability of NSE, the activity of NSE is easily attenuated during the solution preparation process, and the stability of the freeze-drying process itself is poor, which will lead to large errors between different batches

[0009] (3) For doctors to use, the freeze-dried powder needs to be reconstituted and mixed before use, which increases the operation steps and time cost; Can't be reused, it will cause waste

[0010] (4) The instability of NSE increases the difficulty and cost of production for enterprises; it brings inconvenience to doctors; it increases testing costs for patients

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