Method for synthesizing polyhydroxyalkanoate by using residual sludge broth as substrate

A technology of polyhydroxyalkanoate and excess sludge, which is applied in fermentation, sludge treatment, biological sludge treatment, etc., can solve the problem that the synthesis cost of PHAsPHAs cannot meet the requirements of industrialization.

Active Publication Date: 2012-06-20
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0007] Although the above patent documents have discussed and studied the process of using waste to synthesize PHAs from sludge, the conte

Method used

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  • Method for synthesizing polyhydroxyalkanoate by using residual sludge broth as substrate
  • Method for synthesizing polyhydroxyalkanoate by using residual sludge broth as substrate

Examples

Experimental program
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Embodiment 1

[0019] (1) Domestication of sludge

[0020] Take 5L of activated sludge with MLSS of 1g / L in the domesticator, add culture medium solution so that the concentration of each component in the sludge system is: CH 3 COOH 0.1mL / L, CH 3 CH 2 COOH 0.7mL / L, KCl 100mg / L, K 2 HPO 4 ·3H 2 O 50mg / L, NH 4 Cl 85mg / L, MgSO 4 70mg / L, CaCl 2 20mg / L, FeCl 3 ·6H 2 O 5mg / L, peptone 110mg / L, yeast extract 30mg / L, adjust the initial pH value to be neutral, and at an aeration rate of 1L / L / min, aerobic aeration for 21h, stop aeration, discharge mud, control The sludge concentration is 1g / L, 3L of supernatant is discharged after 1 hour of precipitation, and after 18 cycles of "water inflow - aerobic aeration - sludge discharge - sedimentation - effluent" cultivation and domestication, the sludge properties are stable, and the appearance changes from gray to black Gradually turns to light brown.

[0021] (2) Anaerobic fermentation of excess sludge to produce VFAs

[0022] Take 1L MLSS with...

Embodiment 2

[0026] (1) Domestication of sludge

[0027] Take 5L of activated sludge with MLSS of 2g / L in the domesticator, add culture medium solution so that the concentration of each component in the sludge system is: CH 3 COOH 0.6mL / L, CH 3 CH 2 COOH 0.2mL / L, KCl 234mg / L, K 2 HPO 4 ·3H 2 O 150mg / L, NH 4 Cl 194mg / L, MgSO 4 150mg / L, CaCl 2 54mg / L, FeCl 3 ·6H 2 O 10mg / L, peptone 334mg / L, yeast extract 124mg / L, adjust the initial pH value to be neutral, and at an aeration rate of 2L / L / min, aerobic aeration for 21h, stop aeration, discharge mud, Control the sludge concentration at 2g / L, discharge 3L of supernatant after 2 hours of precipitation, and then cultivate and domesticate 20 cycles of "water inflow-aerobic aeration-sludge discharge-sedimentation-water effluent", the sludge properties are stable, and the appearance changes from gray to gray Black gradually turns to beige.

[0028] (2) Anaerobic fermentation of excess sludge to produce VFAs

[0029]For VFAs produced by ana...

Embodiment 3

[0033] (1) Domestication of sludge

[0034] The domestication of sludge is shown in Example 2.

[0035] (2) Anaerobic fermentation of excess sludge to produce VFAs

[0036] For VFAs produced by anaerobic fermentation of excess sludge, see Example 1. The difference is that the sludge concentration MLSS used for anaerobic fermentation to produce acid is 30g / L, and the added sodium hydroxide is 1g. Under the conditions of 60°C and magnetic stirring Treat for 60min. After 36 hours of reaction, volatile fatty acids with a concentration of 2.96 g / L were obtained.

[0037] (3) Synthesis of PHAs from sludge

[0038] See Example 1 for the synthesis of PHAs from the sludge. After 10 hours of accumulation, the concentration of PHAs obtained was 1353 mg / L, the PHAs accounted for 36.2% of the dry weight of the sludge, and the substrate conversion rate was 42%.

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Abstract

The invention provides a method for synthesizing polyhydroxyalkanoate by using a residual sludge broth solution as a substrate, and relates to a synthesis method of polyhydroxyalkanoate. The method comprises the following steps: putting urban aerobic active sludge into a sludge domestication device to domesticate for one domestication period according to a sequence of water intake, aerobic aeration, sludge discharging, precipitating and water discharging, wherein the aeration amount is controlled to be between 1 and 3L/L/min in aerobic aeration, and the domestication is continuously carried out for 18-22 periods to obtain the active sludge stabilized through domestication; putting the urban residual sludge into a reactor, adding sodium hydroxide, carrying out hot alkali pretreatment, and regulating the pH value to be neutral; inoculating anaerobic sludge, stirring to perform anaerobic fermentation, and separating to obtain volatile fatty acid fermentation stock; and putting the active sludge stabilized through domestication into a synthesis reactor, adding the volatile fatty acid fermentation stock, synthesizing polyhydroxyalkanoate by virtue of sludge, regulating the pH value to be neutral, and adding sodium acetate utilized as an external carbon source under an intermittent aeration condition so as to synthesize the polyhydroxyalkanoate.

Description

technical field [0001] The invention relates to a method for synthesizing polyhydroxyalkanoate, in particular to a method for synthesizing polyhydroxyalkanoate with excess sludge fermentation liquid as a substrate. Background technique [0002] With the continuous rise of energy prices and the increasingly prominent environmental problems, the pace of replacing traditional petroleum products with biodegradable materials is gradually accelerating. The production of biodegradable materials polyhydroxyalkanoate (PHAs) by fermentation of pure bacteria has been industrialized, but their production costs are high, and they have not been able to obtain large-scale industrial applications. In the past ten years, the research on the synthesis of PHAs from sludge has been greatly developed; however, compared with traditional petroleum plastics, the price of PHAs synthesized from sludge is still too high because of the high cost of carbon source substrates. Therefore, it is a developm...

Claims

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Application Information

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IPC IPC(8): C12P7/62C02F11/02
CPCY02W10/20
Inventor 李清彪刘正贵何宁王远鹏孙弘王海涛
Owner XIAMEN UNIV
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