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Bacillus subtilis and application thereof to kitchen wastes

A technology of Bacillus subtilis and kitchen waste, applied in the field of microorganisms, can solve the problems of poor effect and low efficiency, and achieve the effect of good treatment effect, good development prospects, efficient, fast and stable treatment method

Active Publication Date: 2016-10-26
BIOMAX ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing biological treatment methods have low efficiency and poor effect

Method used

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  • Bacillus subtilis and application thereof to kitchen wastes
  • Bacillus subtilis and application thereof to kitchen wastes
  • Bacillus subtilis and application thereof to kitchen wastes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and screening of Bacillus subtilis (Bacillus subtilis) CY-4

[0033] The separation process of Bacillus subtilis (Bacillus subtilis) CY-4 of the present invention: take Zhenjiang Jurong planting grape rhizosphere soil 10g, add 90mL sterile water, obtain 10 -1 Dilution, in a shaker at 30°C at 130r / min, cultured for 2-3h, continued to add sterile water to dilute to obtain 10 -5 , 10 -6 , 10 -7 , 10 -8 200uL of the above dilutions were spread on LB plates, cultured at 30°C for 48 hours, and a single colony was picked and inoculated on the slant of LB solid medium for preservation.

[0034] The physiological activity characteristics of Bacillus subtilis CY-4 are as follows:

[0035] a. Morphological characteristics: Gram staining is positive, rod-shaped, with spores, and the spores are oval. On LB solid medium, the surface of the colony is rough and wrinkled, opaque, with irregular edges, milky white to slightly yellow;

[0036] b. Physiological an...

Embodiment 2

[0042] Example 2 Preparation of a biological treatment agent containing Bacillus subtilis and its application in kitchen waste

[0043] Preparation of biological treatment agent containing Bacillus subtilis:

[0044] (1) Culture Bacillus subtilis on LB solid culture at 30°C for 24h; inoculate it in a fermentation liquid shaker flask, and culture it with shaking at 30°C and 130r / min for 24h to form a seed liquid;

[0045] The seed liquid is inoculated into the culture material in the fermenter at a volume ratio of 1:400 for fermentation production. The basic working parameters of the fermenter are: temperature: 30-35°C, stirring speed: 130r / min, pH=6.6-7.2 , dissolved oxygen: 30%, tank pressure: 0.04MPa; stop fermentation after 24 hours of fermentation, then centrifuge at 6000r / min for 10 minutes, take the sediment and dilute it with sterile water to make bacterial agent; the concentration of viable bacteria in the bacterial agent is detected to be 6.3 ×10 9 CFU / mL;

[0046]...

Embodiment 3

[0056] Example 3 Preparation of Biological Treatment Agent Containing Bacillus subtilis Inoculum and Application in Kitchen Waste

[0057] This embodiment is basically the same as Example 2, except that in step (2), the mass ratio of sawdust, bran powder and rice husk is 8:1:1; in step (3), microbial inoculum and carrier The mass ratio is 2:15.

[0058] Weight loss efficiency test of biological treatment agent on food waste after treatment:

[0059] (1) The composition of kitchen waste: the food left in the canteen, remove the large and hard parts such as plastic or bones;

[0060] (2) The biological treatment agent prepared by the above method is applied to the treatment of kitchen waste: preheating operation 6h, ventilation and stirring at the same time; 10kg of food waste to be treated is added in the processor after the pre-operation, and biological treatment agent Mix for aerobic fermentation, and ventilate and stir at the same time; the amount of biological treatment a...

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Abstract

The invention provides bacillus subtilis which is classified and named bacillus subtilis CY-4 and is preserved in Institute of Microbiology, Chinese Academy of Sciences at No. 3 Yard, No. 1, Beichen West Road, Chaoyang District, Beijing, on May 13, 2016, the postal code is 100101, and the preservation number is CGMCC No.12447. The invention further provides application of the bacillus subtilis to kitchen wastes. The bacillus subtilis has the activities of highly-active proteases, amylases and oil hydrolases, degrades glucose-produced acid and contains thermophilic bacteria with high salt resistance and acid resistance, and through the effective combination with a carrier, the weight of the bacillus subtilis put into the kitchen wastes can be quickly and effectively reduced to 85 percent.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a bacillus subtilis, and also relates to the application of the bacillus subtilis in the treatment of kitchen waste. Background technique [0002] Kitchen waste mainly comes from food scraps (kitchen waste) and food residues (swill) from catering operations and residents’ lives. There is a large amount of kitchen waste generated by families, schools, canteens and catering industries in my country. If it is not handled properly, it will pollute the environment, spread diseases, and endanger human health. Food waste has the characteristics of high water content (up to 80-95%), high salinity, high organic matter content, perishable odor and breeding of mosquitoes and flies. The main components are water, sugar, protein, fat, salt and oil, etc. . [0003] At present, the treatment methods of food waste are mainly divided into non-biological treatment (incineration, dehydration, va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14B09B3/00C12R1/125
CPCB09B3/00C12N1/20C12N11/14C12N1/205C12R2001/125
Inventor 周哲刘立鹤王哲李浩张炳如杜国扬
Owner BIOMAX ECOLOGICAL ENG
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