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A kind of Pediococcus lactis and its application in kitchen waste

A technology of Pediococcus lactis and kitchen waste, applied in the field of microorganisms, can solve problems such as poor effect and low efficiency, and achieve the effects of good treatment effect, improved treatment effect and good development prospect.

Active Publication Date: 2020-03-17
BIOMAX ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing biological treatment methods have low efficiency and poor effect

Method used

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  • A kind of Pediococcus lactis and its application in kitchen waste
  • A kind of Pediococcus lactis and its application in kitchen waste
  • A kind of Pediococcus lactis and its application in kitchen waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and screening of Pediococcus acidilactici (Pediococcus acidilactici) CY-10

[0033] The separation process of Pediococcus acidilactici (Pediococcus acidilactici) CY-10 of the present invention: take by weighing Changshu small tomato rhizosphere soil 10g, add 90mL sterile water, obtain 10 -1 Dilution, in a shaker at 30°C at 130r / min, cultured for 2-3h, continued to add sterile water to dilute to obtain 10 -5 , 10 -6 , 10 -7 , 10 -8 200uL of the above dilutions were spread on the MRS solid plate, cultured at 30°C for 48 hours, and a single colony was picked and inoculated on the slant of the MRS solid medium for preservation.

[0034] The physiological activity characteristics of Pediococcus acidilactici (Pediococcus acidilactici) CY-10 are as follows:

[0035] a. Morphological characteristics: Gram staining is positive, the bacteria are spherical in pairs and quadruplets, and the colonies are cultured on MRS solid medium. The colonies are small, o...

Embodiment 2

[0067] Example 2 Preparation of biological treatment agent containing Pediococcus lactic acid bacteria agent and its application in kitchen waste

[0068] Preparation of biological treatment agent containing Pediococcus lactis:

[0069] (1) Cultivate Pediococcus acidilactici CY-10 on MRS solid culture at 30°C for 24 hours; inoculate it in a shaker flask of fermentation liquid, shake it at 30°C and 130r / min for 24 hours to form seed liquid;

[0070] Inoculate the seed liquid into the culture material in the fermenter at a volume ratio of 1:400 for fermentation production. The basic working parameters of the fermenter are: temperature: 30-35°C, stirring speed: 130r / min, pH=6.2-7.2 , dissolved oxygen: 30%, tank pressure: 0.04MPa; stop the fermentation after 24h of fermentation, then centrifuge at 6000r / min for 10min, take the sediment and dilute it with sterile water to make the bacterial agent; the concentration of viable bacteria in the bacterial agent is 7.3 ×10 9 CFU / mL;

...

Embodiment 3

[0084] Example 3 Preparation of Biological Treatment Agent Containing Pediococcus Lactic Acid Bacteria and Application in Kitchen Waste

[0085] This example is basically the same as Example 2, except that the kitchen waste treatment bacteria is Bacillus licheniformis CY-1; Pediococcus lactis and Bacillus licheniformis with a mass ratio of 25:75 are mixed to obtain The mixed microbial agent; the mass ratio of sawdust, bran powder and rice husk is 8:3:3; the mass ratio of microbial agent and carrier is 1:11.

[0086] Weight loss efficiency test of biological treatment agent on food waste after treatment:

[0087] (1) The composition of kitchen waste: the food left in the canteen, remove the large and hard parts such as plastic or bones;

[0088] (2) The biological treatment agent prepared by the above method is applied to the treatment of kitchen waste: preheating operation 6h, ventilation and stirring at the same time; 10kg of food waste to be treated is added in the processo...

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Abstract

The invention provides a Pediococcus lactici, which is classified as Pediococcus acidilactici (Pediococcus acidilactici) CY-10, and is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the address is No. 1, Beichen West Road, Chaoyang District, Beijing No. 3 Institute of Microbiology, Chinese Academy of Sciences, postcode 100101, deposit number: CGMCC NO.12450, deposit date is May 13, 2016. The invention also provides the application of the Pediococcus lactis in the treatment of kitchen waste. The Pediococcus lactis has high activity of protease, amylase, and oil hydrolase, degrades glucose to produce acid, and is a thermophilic bacteria with high salt tolerance and acid resistance. The Pediococcus lactis can effectively improve the treatment effect of other kitchen waste. The weight reduction and synergistic effect can reach more than 5%.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Pediococcus lactis, and also relates to the application of the Pediococcus lactis in the treatment of kitchen waste. Background technique [0002] Kitchen waste mainly comes from food scraps (kitchen waste) and food residues (swill) from catering operations and residents’ lives. There is a large amount of kitchen waste generated by families, schools, canteens and catering industries in my country. If it is not handled properly, it will pollute the environment, spread diseases, and endanger human health. Food waste has the characteristics of high water content (up to 80-95%), high salinity, high organic matter content, perishable odor and breeding of mosquitoes and flies. The main components are water, sugar, protein, fat, salt and oil, etc. . [0003] At present, the treatment methods of food waste are mainly divided into non-biological treatment (incinerat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N11/02B09B3/00C12R1/01C12R1/125
CPCB09B3/00C12N1/16C12N1/20C12N11/02C12N1/205C12R2001/01
Inventor 王哲刘立鹤周哲李浩韦祥银张炳如杜国扬
Owner BIOMAX ECOLOGICAL ENG
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