A kind of Brevibacterium aureus and its application in kitchen waste

A technology for kitchen waste and Brevibacterium, applied in the field of microorganisms, can solve the problems of low efficiency and poor effect, and achieve the effect of good treatment effect, good development prospect, and efficient, fast and stable treatment method.

Active Publication Date: 2020-01-03
BIOMAX ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing biological treatment methods have low efficiency and poor effect

Method used

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  • A kind of Brevibacterium aureus and its application in kitchen waste
  • A kind of Brevibacterium aureus and its application in kitchen waste
  • A kind of Brevibacterium aureus and its application in kitchen waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and screening of Brevibacterium aureum (Brevibacterium aureum) Cl-8

[0032] The separation process of Brevibacterium aureum (Brevibacterium aureum) Cl-8 of the present invention: take by weighing the rhizosphere soil 10g of strawberry planting in Nantong, add 90mL sterile water, get 10 -1 Dilution, in a shaker at 30°C at 130r / min, cultured for 2-3h, continued to add sterile water to dilute to obtain 10 -5 , 10 -6 , 10 -7 , 10 -8 200uL of the above dilutions were spread on LB plates, cultured at 30°C for 48 hours, and a single colony was picked and inoculated on the slant of LB solid medium for preservation.

[0033] The physiological activity characteristics of Brevibacterium aureum (Brevibacterium aureum) Cl-8 are as follows:

[0034] a. Morphological characteristics: Gram staining is positive, contact enzyme reaction is positive, no spores are produced, the bacteria are short rod-shaped, not arranged in chains, and have no motility. The colon...

Embodiment 2

[0065] Example 2 Preparation of biological treatment agent containing Brevibacterium aureus agent and its application in kitchen waste

[0066] Preparation of biological treatment agent containing Brevibacterium aureus:

[0067] (1) Brevibacterium aureum (Brevibacterium aureum) Cl-8 was cultured on the MRS solid medium, cultured at 30°C for 24h; inoculated in a shaker flask of fermentation liquid, shaken at 30°C and 130r / min for 24h to form a seed liquid;

[0068] The seed solution is inoculated into the culture material in the fermenter at a volume ratio of 1:400 for fermentation production. The basic working parameters of the fermenter are: temperature: 30-35°C, stirring speed: 130r / min, pH=6.6-7.2 , dissolved oxygen: 30%, tank pressure: 0.04MPa; stop the fermentation after 24 hours of fermentation, then centrifuge at 6000r / min for 10min, take the sediment and dilute it with sterile water to make the bacterial agent; the concentration of viable bacteria in the bacterial agen...

Embodiment 3

[0082] Example 3 Preparation of biological treatment agent containing Brevibacterium aureus agent and its application in kitchen waste

[0083] This example is basically the same as Example 2, except that the kitchen waste disposal bacteria is Bacillus licheniformis (Bacillus licheniformis) CY-1; Brevibacterium aureus and Bacillus licheniformis with a mass ratio of 25:75 are mixed to obtain The mixed microbial agent; the mass ratio of sawdust, bran powder and rice husk is 6:3:2; the mass ratio of microbial agent and carrier is 1:12.

[0084] Weight loss efficiency test of biological treatment agent on food waste after treatment:

[0085] (1) The composition of kitchen waste: the food left in the canteen, remove the large and hard parts such as plastic or bones;

[0086] (2) The biological treatment agent prepared by the above method is applied to the treatment of kitchen waste: preheating operation 6h, ventilation and stirring at the same time; 10kg of food waste to be treate...

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Abstract

The present invention provides a kind of Brevibacterium aureum, which is classified as Brevibacterium aureum (Brevibacterium aureum) Cl-8, preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the address is No. 1, Beichen West Road, Chaoyang District, Beijing No. 3 Institute of Microbiology, Chinese Academy of Sciences, postcode 100101, deposit number: CGMCC NO.12446, deposit date is May 13, 2016. The invention also provides the application of the Brevibacterium aureus in the treatment of kitchen waste. The Brevibacterium aureus has high activity of protease, amylase, and oil hydrolase, degrades glucose to produce acid, and is a thermophilic bacterium with high salt tolerance and acid resistance. The Brevibacterium aureus can effectively improve the treatment effect of other kitchen waste. The weight reduction and synergistic effect can reach more than 5%.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Brevibacterium aureus, and also relates to the application of the Brevibacterium aureus in the treatment of kitchen waste. Background technique [0002] Kitchen waste mainly comes from food scraps (kitchen waste) and food residues (swill) from catering operations and residents’ lives. There is a large amount of kitchen waste generated by families, schools, canteens and catering industries in my country. If it is not handled properly, it will pollute the environment, spread diseases, and endanger human health. Food waste has the characteristics of high water content (up to 80-95%), high salinity, high organic matter content, perishable odor and breeding of mosquitoes and flies. The main components are water, sugar, protein, fat, salt and oil, etc. . [0003] At present, the treatment methods of food waste are mainly divided into non-biological treatment (incinerat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N11/14B09B3/00C12R1/13C12R1/125C12R1/10C12R1/72
CPCB09B3/00C12N1/16C12N1/20C12N11/14C12N1/205C12R2001/13Y02W30/78
Inventor 刘立鹤王哲周哲张炳如杜国扬韦祥银
Owner BIOMAX ECOLOGICAL ENG
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