Lactobacillus johnsonii, and preparation and application thereof

A technology of Lactobacillus johnsonii and freeze-dried preparations is applied in the field of microorganisms to achieve the effects of improving body immunity, improving intestinal health and reducing the number of harmful bacteria

Inactive Publication Date: 2012-12-26
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, these studies are still preliminary. How to prepare probiotics that are effective and easy to use in sow breeding production still needs further research.

Method used

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  • Lactobacillus johnsonii, and preparation and application thereof
  • Lactobacillus johnsonii, and preparation and application thereof
  • Lactobacillus johnsonii, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Isolation, screening and identification of porcine Lactobacillus johnsonii ZLJ010

[0040] From a standardized breeding pig farm in Beijing, fresh feces of healthy late-pregnancy and lactating sows were collected, put into sterile bags, and immediately brought back to the laboratory in a refrigerated box. Weigh 1 g of feces and add it to 99 mL of sterile saline, and mix well. Take 1mL of the suspension and dilute to 10 with sterile normal saline -5 、10 -6 、10 -7 , 0.1 mL each was spread on MRS solid medium, SL solid medium and LBS solid medium, and cultured anaerobically at 37°C. After 48 hours, the milky white colonies suspected of Lactobacillus were picked for anaerobic pure culture. After 48 h, half of the cultured colonies were picked for H 2 o 2 Enzyme test and Gram stain microscopic examination, all Gram stain positive, H 2 o 2 The strains with negative enzyme test and rod-shaped bacteria can be preliminarily identified as Lactobacillus. Then th...

Embodiment 2

[0055] Embodiment 2 measures the biological characteristics of Lactobacillus johnsonii ZLJ010

[0056] 2.1 Growth curve

[0057] Using OD 600nm The growth characteristics of Lactobacillus johnsonii ZLJ010 were determined based on the index and pH value. see results figure 2 Lactobacillus johnsoni ZLJ010 culture process OD 600nm Curve, image 3 The pH change curve of Lactobacillus johnsonii ZLJ010 culture process.

[0058] Depend on figure 2 , image 3 It can be seen that the OD of Lactobacillus johnsonii ZLJ010 was 600nm The pH value increased rapidly, and the pH value decreased rapidly; during 12~16h, the OD of the bacterial solution 600nm and the change of pH value decreased gradually, indicating that the logarithmic growth phase had been reached; OD after 16h 600nm and pH value did not change significantly, indicating that the bacteria had entered a stable growth period.

[0059] 2.2 Acid resistance test

[0060] 2.2.1 Preparation of artificial gastric juice: 0...

Embodiment 3

[0075] Embodiment 3 prepares Lactobacillus johnsonii preparation

[0076] The lactic acid bacteria preparation is added to the pig’s diet in the form of freeze-dried powder, which is effective and convenient. One is because the freeze-dried lactic acid bacteria preparation is easy to store and has stable activity; the other is because the solid preparation is easy to add to the feed, which is good for mixing uniform. In the production process of freeze-dried preparations, the formula of culture medium, freeze-drying protective agent and freeze-drying process are three important technical links, which directly affect the survival rate of viable bacteria, the number of viable bacteria in freeze-dried preparations and the unit production cost in the production process. . The present invention has carried out technological improvement to these three technical links, has obtained remarkable effect.

[0077] 3.1 Improving the culture medium of Lactobacillus johnsonii ZLJ010

[00...

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Abstract

The invention discloses a Lactobacillus johnsonii, and a preparation and application thereof, and discloses an optimal culture medium for the strain and a preparation method of a freeze-dried preparation of the strain. The microbial preservation number of the Lactobacillus johnsonii ZLJ010 is CGMCC No.5762. The strain is definite in hereditary basis, and has the characteristics of good growth performance and high acid production, acid resistance, heat resistance and bacterium inhibition capabilities. When being used as a feed additive for sows, the freeze-dried preparation of the strain can improve the intestinal tract health of the sows, reduce the number of harmful bacteria in feces, enhance the body immunity and increase the production performance of the sows.

Description

【Technical field】 [0001] The invention belongs to the field of microorganisms, and in particular relates to the application of a Lactobacillus johnsonii ZLJ010 strain and its preparation in microbial feed additives for livestock and poultry. 【Background technique】 [0002] Sows play a very important role in pig production, and the health status and reproductive performance of sows directly affect the production level and economic benefits of pig farms. According to data analysis, the more piglets provided per sow per year, the lower the production cost of sows shared by piglets. The production cost of providing 16 piglets per year is 52% higher than that of providing 22 piglets. Therefore, it is of great significance to improve the reproductive performance of sows through technological innovation. If a high-efficiency probiotic preparation is developed in a targeted manner, the balance of intestinal flora in sows can be well adjusted, the absorption and metabolism of feed n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P31/04C12N1/04A61K9/19A23K1/16C12R1/225A61K35/747
CPCY02A50/30
Inventor 季海峰王四新刘辉张董燕王晶王雅民
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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