Method for preparing caproic acid through two-phase process anaerobic fermentation

A technology of anaerobic fermentation and caproic acid, applied in fermentation and other directions, can solve problems such as inhibition of carbon chain extension process, and achieve the effect of promoting production, efficient coupling, and good research and application prospects.

Active Publication Date: 2018-09-04
江苏道同环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, many researches on the synthesis of MCFAs in the anaerobic fermentation process are more or less affected by the toxicity of acetic acid, butyric acid or hexanoic acid to microorganisms, which inhibits the carbon chain extension process.

Method used

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  • Method for preparing caproic acid through two-phase process anaerobic fermentation
  • Method for preparing caproic acid through two-phase process anaerobic fermentation
  • Method for preparing caproic acid through two-phase process anaerobic fermentation

Examples

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Embodiment 1

[0027] Anaerobic acid production process such as figure 1 shown. Five groups were set up for the acid production phase. The substrate was fruit and vegetable residues. The inoculum sludge was taken from a sewage anaerobic digestion tank of a food processing factory in Wuxi. The inoculation ratio was 2:1 and the total TS was 5%. The initial pH was adjusted to 4, 5, 6, and 7 respectively, and the unadjusted pH system was used as a control experiment. In order to maintain the pH stability, 3 mol·L -1 NaOH solution adjusts the pH. The reaction device is an anaerobic bottle plus a gas bag, with an effective volume of 1L, placed in a 35°C constant temperature water bath.

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Abstract

The invention discloses a method for preparing caproic acid through two-phase process anaerobic fermentation, and belongs to the technical field of waste resource treatment techniques and environment-friendly purification treatment. The method utilizes fruit and vegetable waste as an acidogenic-phase substrate, and the fruit and vegetable waste serving as the acidogenic-phase substrate builds a stable acidogenic fermentation system at the temperature of 35 DEG C and with the pH value being 6.0; the acidogenic-phase fermented liquid serves as a substrate, the caproic acid yield can reach 12.85g.L<-1> under the conditions that the temperature is 30 DEG C, the pH value is 6.8-7.0, the alcohol acid ratio is 4 to 1, that is, the ethyl alcohol additive amount is 17.22g.L<-1>, and thus efficientgeneration of caproic acid is achieved.

Description

technical field [0001] The invention relates to a method for producing caproic acid by two-phase anaerobic fermentation, and belongs to the technical fields of waste resource treatment technology and environmental protection purification treatment technology. Background technique [0002] Caproic acid can be synthesized by adding ethanol or lactic acid as electron donors to the caproic acid-producing bacteria group, and using the reverse β-oxidation pathway of carboxylic acid carbon chain extension (reverse β oxidation). At present, there are few domestic studies on the directional synthesis of caproic acid by anaerobic bacteria, and only some studies focus on the role of pure caproic acid bacteria culture and mixed culture technology in the production of pit mud and liquor. Relevant research work is also mainly focused on the optimization and control of fermentation conditions, and some studies have also discussed the dominant microbial populations in the fermentation proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12P7/52
CPCC12P7/40C12P7/52
Inventor 黄振兴刘春梅余江南阮文权
Owner 江苏道同环境科技有限公司
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