Culture medium for screening enhanced biological phosphorus removal functional bacteria and method for screening enhanced biological phosphorus removal functional bacteria

A technology for biological phosphorus removal and culture medium, which is applied in biochemical equipment and methods, microbial measurement/inspection, and microorganisms, etc. It can solve the problem that the growth of bacteria that is not suitable for enhancing biological phosphorus removal function and the number of bacterial strains that strengthen biological phosphorus removal function are small. , poor targeting and other problems, to achieve the effect of reducing the number of miscellaneous bacteria, the number of miscellaneous bacteria is small, and the nutrients are reasonable

Active Publication Date: 2009-10-21
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the poor pertinence of existing methods for screening and strengthening biological phosphorus removal function bacteria, and the number of bacterial strains to obtain enhanced biological phosphorus removal function bacteria is few and used The medium is not suitable for the growth of bacteria with enhanced biological phosphorus removal function

Method used

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specific Embodiment approach 1

[0009] Specific embodiment 1: In this embodiment, the medium for screening enhanced biological phosphorus removal functional bacteria is four kinds of mediums used for screening in turn by enhanced biological phosphorus removal functional bacteria, and the four mediums are respectively anaerobic Culture medium, aerobic medium, solid separation medium and liquid separation medium; wherein each liter of anaerobic medium is composed of 0.1-0.3g of NaAC, 130-150mg of (NH 4 ) 2 SO 4 , 13~15mg of CaCl 2 2H 2 O, 170~190mg of MgSO 4 ·7H 2 O, 0.2-0.4mL of trace element solution, 0.8-1.2mL of vitamin solution and the rest of distilled water, the pH value of the anaerobic medium is 7-7.2; each liter of aerobic medium is composed of 130-150mg of KH 2 PO 4 , 230~260mg of K 2 HPO 4 , 120~160mg (NH 4 ) 2 SO 4 , 12~16mg of CaCl 2 2H 2 O. 160-200mg of MgSO 4 ·7H 2 O, 0.1-0.6mL of trace element solution, 0.5-1.5mL of vitamin solution and the rest of distilled water, the pH value ...

specific Embodiment approach 2

[0010] Specific embodiment two: the difference between this embodiment and specific embodiment one is the FeCl in the trace element solution in anaerobic medium, aerobic medium, solid separation medium and liquid separation medium 3 -6H 2 O concentration is 1.5g / L, H 3 BO 3 The concentration is 0.15g / L, CuSO 4 ·5H2 O concentration is 0.03g / L, KI concentration is 0.18g / L, MnCl 2 4H 2 O concentration is 0.12g / L, Na 2 MO 4 2H 2 O concentration is 0.06g / L, ZnSO 4 ·7H 2 The concentration of O is 0.12g / L and CoCl 2 ·6H 2 The concentration of O is 0.15g / L. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0011] Specific embodiment three: what this embodiment is different from specific embodiment one or two is that the vitamin solution in the anaerobic medium, aerobic medium, solid separation medium and liquid separation medium is composed of vitamin B with a concentration of 50mg / L 1 , vitamin B with a concentration of 50mg / L 2 , the concentration is 100mg / L vitamin B 6 , vitamin B at a concentration of 1g / L 12 , folic acid at a concentration of 20mg / L, niacin at a concentration of 50mg / L, calcium pantothenate at a concentration of 50mg / L, p-aminobenzoic acid at a concentration of 50mg / L and biotin at a concentration of 20mg / L. Others are the same as in the second embodiment.

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Abstract

The invention provides a culture medium for screening an enhanced biological phosphorus removal functional bacterium and a method for screening the enhanced biological phosphorus removal functional bacterium, and relates to a culture medium for screening a phosphorus removal functional bacterium and a method for screening the phosphorus removal functional bacterium. The culture medium and the method solve the problems that the prior method for screening the enhanced biological phosphorus removal functional bacterium has poor direction, the obtained enhanced biological phosphorus removal functional bacterium has small amount of strains, and the culture medium is not suitable for growth of the enhanced biological phosphorus removal functional bacterium. The culture medium for screening can be an anaerobic culture medium, an aerobic culture medium, a solid separation culture medium and a liquid separation culture medium. The method comprises the following steps: 1, preparing the culture mediums; 2, collecting mud; 3, culturing the anaerobic culture medium; 4, culturing the aerobic culture medium; 6, culturing the anaerobic culture medium; 7, circulating step 4 to step 6 for 2 to 4 times; 8, diluting in constant gradient; 9, culturing the solid separation culture medium; 10, culturing the liquid separation culture medium; 11, repeating step 8 to step 10 for 3 to 8 times; and 12, detecting. The method has strong direction, the functional bacterium obtained by screening has large amount, and the culture medium is suitable for growth of the functional bacterium.

Description

technical field [0001] The invention relates to a medium for screening phosphorus-removing functional bacteria and a method for screening phosphorus-removing functional bacteria. Background technique [0002] The current method for screening enhanced biological phosphorus removal functional bacteria is to place the sludge taken from the enhanced biological phosphorus removal system into a shake flask containing beef extract peptone medium for fermentation and culture, then dilute the cultured bacteria solution, and then apply Distributed in the solid traditional medium for screening to obtain enhanced biological phosphorus removal functional bacteria, but this method also has the following problems: 1. The method is poorly targeted, so during the cultivation process, non-enhanced biological phosphorus removal functional bacteria multiply in large numbers, and miscellaneous bacteria grow. As a result, the proportion of functional bacteria for enhanced biological phosphorus re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12Q1/04
Inventor 任南琪亢涵王秀蘅
Owner HARBIN INST OF TECH
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