A method for rapid screening of phosphorus accumulating bacteria in activated sludge
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A technology of activated sludge and phosphorus-accumulating bacteria, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of poor purpose of screening strains and slow screening process of phosphorus-accumulating bacteria
Inactive Publication Date: 2011-12-07
BEIJING UNIV OF TECH
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[0004] The purpose of the present invention is to solve the problem that the screening process of the existing phosphorus accumulating bacteria is slow, and the purpose of
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Embodiment 1
[0030] (1) Prepare screening medium. The screening medium includes two kinds of aerobic solid medium and anaerobic solid medium, and its composition is as follows: each liter of aerobic solid medium contains 0.3g of NaAC, 6g of beef extract, 12g of tryptone, 6g of NaCl, 0.2g KH 2 PO 4 , 18g of agar powder, 2ml of trace element solution, the remaining distilled water to make up, adjust the pH to 7.0; each liter of anaerobic solid medium contains 3g of NaAC, 6g of beef extract, 12g of tryptone, 6g of NaCl, 18g of agar powder , 2ml trace element solution, the remaining distilled water to make up, adjust the pH to 7.0. The trace element solution is composed of: 0.5g of EDTA and 0.06g of CaCl per liter of distilled water 2 , 0.051g FeSO4 ·7H 2 O, 0.016g CuSO 4 ·5H 2 O, 0.016g CoCl 2 ·6H 2 O, 0.023g ZnSO 4 ·7H 2 O, 0.053 g MnCl 2 4H 2 O, 0.012g (NH 4 ) 6 Mo 7 o 24 4H 2 O. The strain preservation medium is a solid LB medium, and each liter contains 10 g of tryptone,...
Embodiment 2
[0041] (1) Prepare screening medium. The screening medium includes two kinds of aerobic solid medium and anaerobic solid medium, and its composition is as follows: each liter of aerobic solid medium contains 0.1g of NaAC, 4g of beef extract, 8g of tryptone, 4g of NaCl, 0.15g KH 2 PO 4 , 15g of agar powder, 1ml of trace element solution, the remaining distilled water to make up, adjust the pH to 7.0; each liter of anaerobic solid medium contains 1g of NaAC, 4g of beef extract, 8g of tryptone, 4g of NaCl, 15g of agar powder , 1ml trace element solution, the remaining distilled water to make up, adjust the pH to 7.0. The trace element solution is composed of: 0.4g of EDTA and 0.05g of CaCl per liter of distilled water 2 , 0.049g FeSO 4 ·7H 2 O, 0.015g CuSO 4 ·5H 2 O, 0.0163 g CoCl 2 ·6H 2 O, 0.021g ZnSO 4 ·7H 2 O, 0.05 g MnCl 2 4H 2 O, 0.01g (NH 4 ) 6 Mo 7 o 24 4H 2 O. The strain preservation medium is a solid LB medium, and each liter contains 10 g of tryptone...
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Abstract
The invention provides a method for rapidly screening phosphorus-accumulating bacteria in active sludge, belonging to the technical field of sewage treatment and phosphorus removal. The invention relates to a method for rapidly screening high-efficiency phosphorus-accumulating bacteria in a reinforced biological phosphorus removal system. According to the invention, a reinforced biological phosphorus removal technology is an important phosphorus removal technology in sewage treatment, the phosphorus-accumulating bacteria takes a main removal effect in the technology; and the current strain isolation method has the defects that effect is poor and effective strain number is less. In the invention, a photocopy method coordinated with different culture media is utilized, so that bacterial colony passage is performed in the culture conditions alternating between anaerobism and aerobism; and in the passage process, dyeing with Sudan black and toluidine blue is carried out for observation, white-blue plaque selection is carried out for selecting blue plaque strains to be used as high-efficiency phosphorus removal strains. By using the method, the problems of fussy process, slow screeningand poor pertinence caused by a culture medium and culture conditions are solved, and the high-efficiency phosphorus-accumulating bacteria can be rapidly screened out from the active sludge.
Description
technical field [0001] The invention relates to a method for rapidly screening high-efficiency phosphorus-accumulating bacteria in an enhanced biological phosphorus removal system. Background technique [0002] Enhanced biological phosphorus removal technology is an important phosphorus removal technology in sewage treatment. If the phosphorus content in the water body exceeds the standard, it will cause algae in the water body to multiply and form algal blooms, which will deteriorate the water body condition. Therefore, how to reduce the discharge of phosphorus in the environmental water body is an important measure to prevent the eutrophication of the water body. The enhanced biological phosphorus removal technology is to use the microorganisms in the sludge to overabsorb the phosphorus element in the water body under specific culture conditions, and then reduce the phosphorus content in the water by discharging the remaining sludge. It can be seen that the type and quan...
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