Streptomyces hygroscopicus and use thereof

A technology of Streptomyces hygroscopicus and strains, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of many fermentation by-products, difficult chromatographic separation, low product yield, etc., and achieve less fermentation by-products, reduce Difficulty, the effect of stabilizing production capacity

Active Publication Date: 2009-07-22
HANGZHOU HUADONG MEDICINE GRP PHARMA RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

General sirolimus-producing bacteria, the fermentation unit is only about 200ug / ml, and the fermentation by-products are more, resulting in more difficult chromatographic separation during subsequent extraction, more complicated work, and a product yield of less than 20%.

Method used

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  • Streptomyces hygroscopicus and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: The culture characteristics of the bacterial strain whose preservation number is CCTCC NO: M 207200

[0032] The medium recommended by the International Streptomyces Program and the medium recommended by Waksman were used to describe the culture characteristics. The strain with the preservation number CCTCC NO: M 207200 was cultured at 25-30°C for 7, 14, and 28 days to observe the results. The description of color adopts "chromatogram" published by Science Press in 1957.

[0033] The preservation number is CCTCC NO: M 207200. The air filament growth of the bacterial strain is poor-medium, white-medium gray (IX44'), and black water-absorbing spots appear after 2 to 3 weeks of cultivation. The base filaments are well developed, colorless-beige (Ic34') on inorganic medium, and light mango yellow-light turtle gray (Ia33'-Ic54') on organic medium. There are beige soluble pigments only on glycerol-calcium malate agar.

[0034] Culture characteristics of the stra...

Embodiment 2

[0036] Example 2: Physiological and biochemical characteristics

[0037] Physiological and biochemical properties used standard methods in the classification of Streptomyces.

[0038] The strain with the preservation number CCTCC NO: M 207200 can utilize cellulose, hydrolyze starch, liquefy gelatin, freeze and coagulate milk, reduce nitrate, and do not produce H 2 S; growth pH 5 ~ 9, growth temperature 15 ~ 40 ℃.

Embodiment 3

[0039] Example 3: Carbon source utilization

[0040] The carbon source utilization test used Chapeauer and Goose No. 1 agar without carbon source as the basic medium, added 2% of various carbon sources respectively, and inserted into the strain with the preservation number of CCTCC NO: M 207200, cultured at 25-30°C for 10 , 20 days observation results.

[0041] The strain with the preservation number CCTCC NO: M 207200 can utilize most carbon sources such as glucose, mannose, fructose, sucrose, glycerin, raffinose, starch, etc., and does not utilize carbon sources such as sorbose and rhamnose.

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Abstract

The invention discloses a streptomyces hygroscopicus and applications thereof. The streptomyces hygroscopicus strain is preserved in China Center for Type Culture Collection with CCTCC NO. of M 207200 and the preservation date of December 14th, 2007. The strain with CCTCC NO. of M 207200 is a currently-separated natural strain with high sirolimus yield and excellent fermentation performance. The fermentation yield of the sirolimus-producing strain can reach over 600ug/ml, has relatively less fermentation byproducts, lowers post extraction difficulty, can reach the product yield of over 30 percent, and is applicable to industrial production.

Description

technical field [0001] The invention relates to a microorganism and its application, in particular to a Streptomyces hygroscopicus and its application in preparing sirolimus. Background technique [0002] Immunosuppressants are used to prevent rejection in organ transplantation, that is, to interfere with the receptor's recognition of foreign antigens and the removal of non-self cells. Since the first successful kidney transplant between identical twins in 1954, there have been many major breakthroughs in medical science research on organ transplantation over the past 40 years. The clinical use of cyclosporine A (cyclosporine A) in 1978 ushered in a new era for organ transplantation. The 2-year survival rate of most patients with kidney transplantation has exceeded 80%, and the survival rate of patients has also improved. [0003] Sirolimus (Sirolimus) is a metabolite of Streptomyces hygroscpicus. It is a new type of potent immunosuppressant. It is a lipophilic 35-membered...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/18C12R1/465
Inventor 朱健卿亚丽陈晓霞
Owner HANGZHOU HUADONG MEDICINE GRP PHARMA RES INST
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