Low-temperature resisting method for festuca arundinacea by using complex microbial inoculants

A technology of compound microbial bacterial agent and tall fescue, which is applied in the field of air-dried sludge bacterial agent and tall fescue anti-low temperature, and can solve rare problems

Inactive Publication Date: 2014-07-23
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few reports on the comprehensive evaluation of the greening of the lawn and the comprehensive evaluation of the appearance of the lawn after the greening of the sludge microbial agent

Method used

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  • Low-temperature resisting method for festuca arundinacea by using complex microbial inoculants
  • Low-temperature resisting method for festuca arundinacea by using complex microbial inoculants
  • Low-temperature resisting method for festuca arundinacea by using complex microbial inoculants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] (1) Cultivation of strains

[0109] Weigh fresh sludge and prepare 10 by dilution separation method -3 、10 -4 、10 -5 、10 -6 sludge suspension. According to the isolated microorganisms, take different concentrations of sludge suspension (fungi take 10 -3 、10 -4 , Actinomycetes take 10 -4 、10 -5 , bacteria take 10 -5 、10 -6 ), shake well and pipette 0.2 mL into the prepared plate. Fungi use Martin's agar plate; actinomycetes use Gao's No. 1 agar plate; bacteria use beef extract peptone plate. Spread evenly with a sterilized applicator, repeat 3 times for each treatment, and incubate at 28°C. According to the shape, size, color and growth speed of the colonies in the plate, pick as many single colonies as possible from each sample and streak for purification. The strains were classified and counted according to their colony culture traits and morphological characteristics. The dominant strains were determined and stored under serial numbers pending further ide...

Embodiment 2

[0119] A compound microbial bacterial agent is characterized in that it is made up of following composition:

[0120] 100-fold dilution of Pseudomonas stutzeri + Trichoderma reesei + Streptomyces flavum;

[0121] Wherein Pseudomonas stutzeri: Trichoderma reesei: the volume ratio of Streptomyces flavus is 1:1:1

[0122] Follow the steps below:

[0123] (1) Selection of materials:

[0124] Select tall fescue with plump and uniform grains ( Festuca arundinacea L.) The seeds are the test materials; the test soil is common garden soil;

[0125] (2) Test method

[0126] Preparation of compound microbial bacterial agent: Select Pseudomonas stutzeri, Trichoderma reesei and Streptomyces griseus, purify and culture the isolated strains for 2 generations, take the activated strains, and insert them into a container containing 200 mL of liquid medium. In a triangular flask, cultivate at a suitable temperature of 180 r / min, select 600 nm wavelength for turbidity measurement, take the...

Embodiment 3

[0133] A compound microbial bacterial agent is characterized in that it is made up of following composition:

[0134] 200-fold dilution of Pseudomonas stutzeri + Trichoderma reesei + Streptomyces flavum;

[0135] Wherein Pseudomonas stutzeri: Trichoderma reesei: the volume ratio of Streptomyces flavus is 1:1:1

[0136] Follow the steps below:

[0137] (1) Selection of materials:

[0138] Select tall fescue with plump and uniform grains ( Festuca arundinacea L.) The seeds are the test materials; the test soil is common garden soil;

[0139] (2) Test method

[0140] Preparation of compound microbial bacterial agent: Select Pseudomonas stutzeri, Trichoderma reesei and Streptomyces griseus, purify and culture the isolated strains for 2 generations, take the activated strains, and insert them into a container containing 200 mL of liquid medium. In a triangular flask, cultivate at a suitable temperature of 180 r / min, select 600 nm wavelength for turbidimetric measurement, take...

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Abstract

The invention discloses a low-temperature resisting method for festuca arundinacea by using air-dried sludge inoculants, which is implemented by comprising components including pseudomonas stutzeri, trichoderma reesei and yellow streptomyces 100-times diluent or pseudomonas stutzeri, trichoderma reesei and yellow streptomyces 200-times diluent. Development results of the invention show that the application of the complex microbial inoculants of pseudomonas stutzeri, trichoderma reesei and streptomyces griseoflavus can significantly improve the height, the aboveground biomass and the chlorophyll content of festuca arundinacea plants, and improve the cold resistance thereof. The regreening time of festuca arundinacea to which the complex microbial inoculants are applied is about five days earlier than that in a blank, and the comprehensive index of lawn appearance of regreened festuca arundinacea is also significantly higher than that in the blank, which fully shows the application potential of the complex microbial inoculants in practice.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to a method for applying an air-dried sludge microbial agent to resist low temperature of tall fescue. Background technique [0002] As an important part of urban landscaping, lawn has been gradually known for its ecological benefits and economic benefits. First of all, as an ecosystem, the lawn is a huge carbon source storage, and the lawn vegetation can assimilate CO in the atmosphere 2 , and lawn soil can also absorb CO in the atmosphere 2 . Lal's research shows that grassland soils in the world can absorb and deposit 0.01-0.3Gt of carbon per year. thus reducing CO in the air 2 to reduce the greenhouse effect to a certain extent. Secondly, the dense surface covering layer and grass root layer formed by the lawn have a good effect on preventing soil erosion. Third, the lawn has the ability to absorb SO in the air 2 and NO 2 And other toxic gases release oxyg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P21/00A01N63/00
Inventor 赵树兰多立安王志旭
Owner TIANJIN NORMAL UNIVERSITY
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