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Novel culture medium for producing monensin by fermenting streptomyces cinnamonensis and culture method

A technology of Streptomyces cinnamonis and fermentation medium, which is applied in the field of fermentation, can solve the problems of high market price, carbonization of glucose, long fermentation and cultivation cycle, etc., and achieves the effect of increasing the fermentation unit and reducing the production cycle

Inactive Publication Date: 2014-07-23
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]2 Glucose is added to the culture medium for domestic fermentation and production of monensin, and after high temperature sterilization, it is easy to cause part of the glucose to be carbonized, reduce the total sugar content, and affect the quality of the fermentation broth
[0006]3 The procurement cost of raw materials required for monensin fermentation production is relatively high
The organic nitrogen sources commonly used in monensin medium are mainly peanut cake powder (or soybean cake powder), peptone, and yeast powder, and their market prices are relatively high
[0007]4 The domestic monensin fermentation and cultivation period is relatively long, generally over 300 hours, resulting in high energy consumption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Primary seed medium preparation : Seed tank volume 1.5m 3 , the effective volume is 1m 3 .

[0067] Bran 1kg, maltose 5kg, fish meal 3kg, corn steep liquor 5L, corn gluten powder 1kg, beer yeast extract 4kg, ammonium sulfate 1kg, light calcium carbonate 1kg, olive oil 5L.

[0068] Sterilize the primary seed medium and cool it down to 25°C, keep the pressure with sterile air, and then transfer the cultured Streptomyces cinnamomi mother bottle fermentation broth into the primary seed tank for cultivation under flame protection, and inoculate The volume is controlled at 0.3L. Amino nitrogen of primary seed medium after sterilization: 51mg / 100ml, dissolved phosphorus: 104ug / ml, fat: 12g / L, pH: 7.3.

[0069] Primary seed cultivation: tank pressure 0.03~0.05MPa; tank temperature 31~33℃; air flow: 0~5h, using air stirring instead of mechanical stirring; 6h~transplanting: 50m 3 / h; stirring speed 60r / min.

[0070] The primary seed cultivation is completed, the bacterial ...

Embodiment 2

[0089] Primary seed medium preparation : Seed tank volume 1.5m 3 , the effective volume is 1m 3 .

[0090] Bran 2kg, maltose 6kg, fish meal 4kg, corn steep liquor 6L, corn gluten powder 2kg, beer yeast extract 5kg, ammonium sulfate 2kg, light calcium carbonate 2kg, rapeseed oil 6L.

[0091] Sterilize the primary seed medium and cool it to 26°C, keep the pressure with sterile air, and then transfer the cultured Streptomyces cinnamomi mother bottle fermentation liquid into the primary seed tank for cultivation under flame protection, and inoculate The volume is controlled at 0.3L. Amino nitrogen of primary seed medium after sterilization: 53mg / 100ml, dissolved phosphorus: 112ug / ml, fat: 14g / L, pH: 7.2.

[0092] Primary seed cultivation: tank pressure 0.03~0.05MPa; tank temperature 31~33℃; air flow: 0~5h, using air stirring instead of mechanical stirring; 6h~transplanting: 52m 3 / h; stirring speed 65r / min.

[0093] The primary seed cultivation is completed,...

Embodiment 3

[0112] Primary seed medium preparation : Seed tank volume 1.5m 3 , the effective volume is 1m 3 .

[0113] Bran 3kg, maltose 7kg, fish meal 5kg, corn steep liquor 7L, corn gluten powder 3kg, beer yeast extract 6kg, ammonium sulfate 3kg, light calcium carbonate 3kg, rapeseed oil 7L.

[0114] Sterilize the primary seed medium and cool it to 27°C, keep the pressure with sterile air, and then transfer the cultured Streptomyces cinnamomi mother bottle fermentation broth into the primary seed tank for cultivation under flame protection, and inoculate The volume is controlled at 0.3L. Amino nitrogen of primary seed medium after sterilization: 55mg / 100ml, dissolved phosphorus: 124ug / ml, fat: 18g / L, pH: 7.0.

[0115] Primary seed cultivation: tank pressure 0.03-0.05MPa; tank temperature 31-33°C; air flow: 0-5h, using air stirring instead of mechanical stirring; 6h-transplanting: 55m 3 / h; stirring speed 70r / min.

[0116] The primary seed culture is completed, the...

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Abstract

The invention relates to a novel culture medium for producing monensin by fermenting streptomyces cinnamonensis and a culture method. The fermentation unit of the monensin can be increased, the fermentation period can be shortened and the fermentation cost can be reduced by use of the formula of the culture medium and a fermentation control process, and moreover, the source of raw and auxiliary materials is avoided from environmental influence to the utmost extent, the sufficient supply of the raw and auxiliary materials is guaranteed, and then stable high-efficiency production of the monensin is realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, and in particular relates to a novel culture medium and a culture method for fermenting and producing monensin by Streptomyces cinnamomi. Background technique [0002] Monensin, also known as monensin, monensic acid, and rumenin, is a fat-soluble antibiotic isolated from a strain of Streptomyces cinnamomi by the Eli lilly company of the United States in 1967. It is a broad-spectrum anticoccidial drug, which has high antibacterial activity against Gram-positive bacteria such as Staphylococcus aureus, Streptococcus, Bacillus subtilis and Treponema hyodysenteriae. It is mainly used to prevent coccidiosis and swine dysentery in chickens, rabbits, calves, and lambs, and can promote their growth and improve feed utilization. [0003] At present, the fermentation production of monensin in China adopts a three-stage fermentation mode. The main problems of this method are as follows: [0004] 1 Th...

Claims

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Application Information

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IPC IPC(8): C12P17/16C12R1/465
Inventor 任勇王义
Owner 宁夏泰瑞制药股份有限公司
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