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Biocontrol bacterium and application of biocontrol bacterium to preventing and controlling cotton blight and cotton greensickness

A technology of bio-control bacteria and bio-control fungicides, applied in the direction of application, microbial-based methods, chemicals for biological control, etc., to achieve significant control effects, good control effects, and good application and development prospects

Inactive Publication Date: 2013-01-16
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the registered biocontrol bacteria for the control of Verticillium wilt of cotton have not been reported, but there are commercial products of biocontrol agents for the control of Verticillium wilt of other crops

Method used

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  • Biocontrol bacterium and application of biocontrol bacterium to preventing and controlling cotton blight and cotton greensickness
  • Biocontrol bacterium and application of biocontrol bacterium to preventing and controlling cotton blight and cotton greensickness
  • Biocontrol bacterium and application of biocontrol bacterium to preventing and controlling cotton blight and cotton greensickness

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The separation of embodiment one biocontrol bacteria

[0019] Using 0-30cm soil samples from Xinjiang Qijiaojing Salt Lake (altitude 1584.49m, north latitude 43.48°, east longitude 91.48°), eight conventional culture media containing 5%, 10%, 15%, 20% and 25% sodium chloride were used (GW1 medium, GPY medium, chitosan asparagine medium, glycerol arginine medium, glycerol inorganic salt medium, mannitol inorganic salt medium, ISP-4 medium and PY medium), using Dilution plate method (weigh 1 g of soil sample and put it into 9 mL of sterile aqueous solution, shake fully, and make 10 -1 Suspension. Aspirate 0.1 mL of the suspension, evenly spread it on the pre-prepared separation medium plate, and culture it upside down at 37°C. The petri dish is sealed with plastic wrap to reduce the water loss of the culture medium and prolong the culture time. ), a total of 35 strains of actinomycetes were isolated from 7 genera, mostly distributed in Streptomyces and Pseudomonas. Act...

Embodiment 2

[0020] Embodiment two identification of biocontrol bacteria

[0021] In order to further confirm the taxonomic status of the biocontrol fungus 42561, the present invention uses traditional classification methods and molecular classification methods to classify and identify the strain.

[0022] 1 Culture medium for morphology observation

[0023] Inorganic salt starch agar (ISP-4) medium: starch 10.00 g, (NH 4 ) 2 SO 4 4.00 g, MgSO 4 2.00 g, K 2 HPO 4 2.00 g, CaCO 3 1.00 g, 40.00 g of Nacl, 18.00 g of agar, and distilled water were dissolved to 1000ml, pH 7.2-7.4, and sterilized at 121°C for 30 minutes.

[0024] 2 primers

[0025] Synthesized by Saiyin Biotechnology (Shanghai) Co., Ltd. using actinomycetes universal primers. The sequences are: 27F (5'-AGAGTTTGATCCTGGCTC-3') and 1492R (5'-CGGCTACCTTGTTACGACTT-3')

[0026] 3 Test method

[0027] 3.1 Morphological observation

[0028] Ordinary microscope observation: using insert method, 37 ℃ culture biocontrol bac...

Embodiment 3

[0048] Example three biological antibacterial 42561 antibacterial activity

[0049] 1 Pathogens tested

[0050] Cotton Verticillium dahliae pathogen: Verticillium dahliae ( Verticillium dahliae ), cotton wilt pathogen: Fusarium oxysporum cotton-specific type ( Fusarium oxysporum sp. vasinfectum ) provided by the Xinjiang Production and Construction Corps Tarim Basin Biological Resources Protection and Utilization Key Laboratory.

[0051] 2 Plants and agents tested

[0052] Test plants: Cotton (Xinluzhong 36)

[0053] Treatment agent: fermentation broth of biocontrol bacteria 42561

[0054] Control agent: carbendazim wettable powder (Shandong Dongxin Biological Pesticide Co., Ltd.)

[0055] 3 media

[0056] Potato Dextrose Agar Medium (PDA): Take 200g of peeled potatoes, cut into small pieces, boil in water for 30min, filter with four layers of gauze, add 20g of glucose to the filtrate, 18g of agar, distilled water to 1000mL, pH natural. After filling the conical fl...

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Abstract

The invention discloses Streptomyces rochei 42561 which is preserved in the general microorganism center of the China Committee for Culture Collection of Microorganisms (CCCCM), with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.6556. The bacteriostatic and prophylactic actions of the biocontrol bacterium 42561 are comprehensively tested according to a cup-plate method, a mycelial growth rate inhibition method, a spore germination inhibition method, greenhouse pot culture and field tests; and the testing results show that the strain has a remarkable prevention effect on cotton blight and cotton greensickness, can be used as an effective biological pesticide preparation agent and has excellent application and development prospects.

Description

technical field [0001] The invention relates to the field of biological control, in particular to Streptomyces loucherii ( Streptomyces rochei ) and its biological control purposes. Background technique [0002] Cotton fusarium wilt and cotton verticillium wilt are the most important diseases that harm cotton production. In recent years, cotton fusarium wilt and cotton verticillium wilt have become increasingly serious in cotton areas of my country. However, there is no effective medicament for controlling cotton fusarium wilt and cotton verticillium wilt at present. Searching for biological pesticides from microbial resources is one of the effective ways, and the agricultural antibiotics produced by actinomycetes are the most important sources. The development and promotion of biological pesticides such as Jinggangmycin, Abamectin and Qinlingmycin have fully proved the rationality and superiority of screening economical and efficient agricultural antibiotics from the meta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
Inventor 张利莉柳成宾贾晓宇李艳宾范瑛阁曾红张飞万传星
Owner TARIM UNIV
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