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Composite biological seed coating agent used for controlling vegetable soil-borne diseases and preparation and application methods thereof

A technology for soil-borne diseases and biological species, applied in botany equipment and methods, chemicals for biological control, biocides, etc. , poor film-forming effect, etc., to achieve the effect of low cost, uniform seed coating and strong adhesion

Active Publication Date: 2014-08-13
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this preparation method needs to be processed by high-pressure spray drying, and the production cost is high and the process is complicated.
The Chinese invention patent application with the publication number CN102972447A and the publication date of March 20, 2013 discloses a biological seed dressing, which includes bentonite as a carrier medium, starch and hydroxymethylcellulose as an adhesive, and soil beneficial microorganisms Bacillus subtilis, Bacillus licheniformis and Streptomyces Loucheri, but this biological seed dressing has the following defects: diffusion, poor film-forming effect, uneven coating treatment, slow colonization speed of Bacillus subtilis in plant rhizosphere, The amount of colonization is small, and the effect of use is unstable
Traditional seed coating agents are composed of chemical pesticides added to film materials, which are likely to cause environmental pollution and pesticide residues after use, which is not conducive to the improvement of soil microbial groups. Therefore, the development of new biological seed coating agents is the future development direction of seed treatment technology

Method used

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  • Composite biological seed coating agent used for controlling vegetable soil-borne diseases and preparation and application methods thereof
  • Composite biological seed coating agent used for controlling vegetable soil-borne diseases and preparation and application methods thereof
  • Composite biological seed coating agent used for controlling vegetable soil-borne diseases and preparation and application methods thereof

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Experimental program
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Effect test

preparation example Construction

[0025] The preparation method of the above-mentioned compound biological seed coating agent for preventing and treating soil-borne diseases of vegetables comprises the following steps:

[0026] (1) Beneficial microorganisms Bacillus subtilis and Streptomyces Loucherii were isolated from vegetable rhizosphere soil. The preparation is prepared according to the conventional microbial culture method: strain → inclined plane → shake flask → seed tank → fermenter → fermented liquid. Bacillus subtilis fermentation liquid is filtered by ultrafiltration membrane and concentrated to a bacterial content of >10 11 cfu / g. Streptomyces louchei was concentrated by ultrafiltration membrane to >70PMV / g;

[0027] (2) Stir and mix the fermented liquid of Bacillus subtilis and Streptomyces loucherii described in (1) in a reaction kettle at a weight ratio of 2:1 to obtain a mixed liquid of beneficial microorganisms;

[0028] (3) Add the sodium hydroxymethylcellulose, sodium lignosulfonate, chit...

Embodiment 1

[0033] The synergistic effect of Bacillus subtilis and Streptomyces loucherii was determined by co-toxicity coefficient method. Accurately weigh Bacillus subtilis (10 11 cfu / g) and Streptomyces Loucheri (70PMV / g), according to the ratio of Bacillus subtilis: Streptomyces Loucheri: 1:0, 1:1, 3:2, 2:1, 5:2, Mixed bacterial solutions were prepared in different ratios of 3:1. On the ultra-clean workbench, dilute the bacterium solution in 7 different proportions with sterile water into 5 concentration gradients of the drug solution, use a micropipette to draw 4mL of the drug solution from low concentration to high concentration, and add it to the previously melted and sterilized solution respectively. Shake well, then pour an equal amount into four sterilized petri dishes with a diameter of 9 cm, and after cooling, the corresponding concentration of drug-containing culture base plate. In the experiment, the treatment without drug was used as blank control. At the edge of the co...

Embodiment 2

[0037] According to the synergistic combination ratio of Bacillus subtilis in embodiment 1: Streptomyces Louchei=2: 1, add 30% Bacillus subtilis fermentation concentrate (number of live bacteria ≥ 10 11 cfu / g), 15% Streptomyces Louchei fermentation concentrate (biomass ≥ 70PMV / g), fully stirred and mixed.

[0038] Add 3.5% sodium hydroxymethylcellulose, 0.5% sodium lignosulfonate, 1% chitosan, 0.2% sodium benzoate, and 0.1% rhodamine to the mixture of Bacillus subtilis and Streptomyces loucheri, and continue stirring mix.

[0039] Dissolve 2% polyvinyl alcohol in an appropriate amount of hot water at 60-70°C, cool to room temperature, add to the above mixture, add distilled water to make up to 100%, continue to stir and mix to prepare the compound biological seed coating agent.

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Abstract

The invention discloses a composite biological seed coating agent used for controlling vegetable soil-borne diseases and preparation and application methods thereof; the composite biological seed coating agent comprises bacillus subtilis and streptomyces rochei, also contains water as a carrier medium, sodium carboxymethyl cellulose as an adhesive, sodium lignin sulfonate as a dispersing agent, chitosan and polyvinyl alcohol as a film forming agent, sodium benzoate as a preservative and rhodamine as a colorant; the preparation method is as follows: (1) respectively preparing bacillus subtilis and streptomyces rochei fermentation liquids; (2) stirring and mixing the bacillus subtilis and streptomyces rochei fermentation liquids in a ratio of 2:1; (3) proportion ally adding the sodium carboxymethyl cellulose, the sodium lignin sulfonate, the chitosan, the polyvinyl alcohol, the sodium benzoate and the rhodamine, and finally complementing the margin with the water.; and the application method is as follows: coating, root dipping and root irrigation. The composite biological seed coating agent is obvious in disease resistance effect and long in effective period, has the effects of saving seeds, strengthening seedlings and the like, and is free of environmental pollution.

Description

technical field [0001] The present invention relates to a compound biological seed coating agent for preventing and treating soil-borne diseases of vegetables, in particular to a compound biological seed-coating agent containing two kinds of soil beneficial microorganisms and capable of preventing and controlling soil-borne diseases of vegetables, and the preparation of the biological seed-coating agent and how to use it. Background technique [0002] Vegetable soil-borne diseases are mainly caused by pathogens such as Fusarium, Phytophthora, Pythium, Rhizoctonia solani and Pseudomonos. It is a plant vascular bundle disease that is widespread, harmful, and difficult to control. There are many types of diseases, mainly melons, solanaceous fruits, beans, leafy vegetables blight, fusarium wilt, damping-off, sclerotinia, anthracnose, root rot and so on. The occurrence of soil-borne diseases leads to serious reduction or even failure of vegetable production, seriously affecting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P3/00
Inventor 徐健刘琴李传明韩光杰刘怀阿祁建杭孙俊
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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