Methods and compositions to enhance plant breeding

a technology of compositions and plants, applied in the field of methods, can solve problems such as unadjusted areas, and achieve the effects of increasing grain yield, enhancing agronomic and transgenic traits, and increasing oil

Inactive Publication Date: 2006-12-14
MONSANTO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0011] The present invention includes a method for breeding of a crop plant, in particular a soybean or corn plant with enhanced agronomic and transgenic traits comprising a preferred T-type genomic region. A transgene of the T-type genomic region is further defined as conferring a preferred property like herbicide tolerance, disease resistance, insect or pest resistance, altered fatty acid, protein or carbohydrate metabolism, increased grain yield, increased oil, increased nutritional content, increased growth rates, enhanced stress tolerance, or altered morphological characteristics, or any combination of these.

Problems solved by technology

This is an area that has not been adequately addressed in current plant breeding with transgene insertion events.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Identification of Haplotypes

[0104] This example illustrates identifying soybean haplotypes useful in databases for practicing the methods of this invention. The chromosomes of soybean were divided into haplotypes by following the hereditability of a large set of makers. Allelic forms of the haplotypes were identified for a set of 4 haplotypes which are listed in Table 1. With reference to Table 1, a haplotype mapped to a genomic location is identified by reference, for example C8W6H5 refers to chromosome 8, window 6 in that chromosome and haplotype 5 in that window (genomic region); SEQ_ID provides reference to the sequence listing and the marker ID number is an arbitrary identifying name for a DNA amplicon associated with the a marker locus; START_POS refers to the start position of the marker in the DNA amplicon; HAP allele refers to the nucleotide of an SNP / Indel marker at the Start position where * indicates a deletion of an Indel; “other marker states” identifies another nucle...

example 2

Preparation of a Database with Agronomic Traits and Haplotypes

[0105] This example illustrates the preparation of a database useful in a method of this invention. With reference to Table 2 the database comprises computed values of agronomic traits, for example, yield, maturity, plant height, and lodging, for the specific allelic soybean haplotypes and the haplotype frequency in a set of breeding lines. Other traits can be measured, for example, yield of a grain, seed, fruit, fiber, forage, oil; or an agronomic trait, for example, pest resistance such as disease resistance, insect resistance, nematode resistance, or improved growth rate, and stress tolerance; or an improved processed product of the plant, for example, fatty acid profile, amino acid profile, nutritional content, fiber quality and a database compiled for the values of each haplotype for these other traits. The agronomic trait values of these haplotypes represent the predicted population change in mean value for the tra...

example 3

Use of Breeding Values

[0110] The haplotype regions and breeding values of each were determined for four haplotype regions in which an insect tolerance gene was inserted into the genome of a soybean plant. The relative breeding value for each haplotype regions is shown in Table 3, the definitions of the measurements are the same as described in Example 2. The table is a database for determining the haplotype and its breeding value in which an insect tolerance gene was inserted (a T-type). A transgenic event comprising the T-type is selected using the database information. A particular event, GM—19459, contains the T-type of the insect tolerance gene associated with C6W4H1 haplotype that is a favorable haplotype for maturity.

TABLE 3The calculated breeding values for yield, maturity, plantheight, and lodging of four haplotypes for the insect tolerantsoybean events. The frequency of the haplotype in the germplasmwas estimated from 2589 soybean lines.YieldPlant(Bushels / MaturityheightL...

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Abstract

The present invention provides breeding methods and compositions to enhance the germplasm of a plant. The methods describe the identification and accumulation of transgenes and favorable haplotype genomic regions in the germplasm of a breeding population of crop plants.

Description

[0001] This application claims the benefit of U.S. Provisional Application No. 60 / 685,584, filed May 27, 2005, the entire text of which is specifically incorporated by reference hereinBACKGROUND OF THE INVENTION [0002] 1. Field of the Invention [0003] The invention relates to the field of plant breeding and plant biotechnology, in particular to a transgene inserted into genetic linkage with a genomic region of a plant, and to the use of the transgene / genomic region to enhance the germplasm and to accumulate other favorable genomic regions in breeding populations. [0004] 2. Description of Related Art [0005] Breeding has advanced from selection for economically important traits in plants and animals based on phenotypic records of the individual and its relatives to the use of molecular genetics to identify genomic regions that contain the valuable genetic traits. Information at the DNA level has lead to faster genetic accumulation of valuable traits into a germplasm than that achieved...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01H1/02A01H5/00A23L11/00
CPCA01H1/02A01H1/04A01H5/10A01N57/20C12N15/8261C12N15/8275C12N15/8286C12N15/821A01N2300/00Y02A40/146C12N15/11C12Q1/6876A01H6/542
Inventor BULL, JASONBUTRUILLE, DAVIDEATHINGTON, SAMEDWARDS, MARLINGUPTA, ANJUJOHNSON, RICHARDKENNARD, WAYNERINEHART, JENNIFERWU, KUNSHENG
Owner MONSANTO TECH LLC
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