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A seedling cultivation method for Sargassum vachellianum greville by using tissue culture

A technique for culturing Sargassum waschii and tissue, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of small breeding quantity, long breeding time, and limited seedling production, and achieve easy preservation and improvement The effect of ecological environment and great preservation

Inactive Publication Date: 2013-11-13
SHANGHAI OCEAN UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sexual reproduction of Sargassum was affected by the amount of eggs laid and the season, the seed production was severely limited, and the breeding time was long and the breeding quantity was small, so it was difficult to meet the needs of sea multiplication or artificial cultivation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] 1. The cultivation of seedlings:

[0030] Collect the immature wild Sargassum varschii algae from the reproductive receptacle, and temporarily raise them under the conditions of a temperature of 18°C, a light intensity of 1000-2000lx, and a photoperiod of 10L:14D, and replace the culture medium every 10 days until the reproductive receptacle grows. After distinguishing between male and female, cut off the side branches of the genital receptacle, promote maturation under the conditions of temperature 24°C, light intensity 2000-3000lx, photoperiod 14L:10D, and replace the culture medium every 3 days to promote egg release. Place the collected fertilized eggs at a temperature of 18°C, light intensity of 1000-1500lx, and photoperiod of 10L:14D for static culture. The culture medium was changed every 3 days for the first 30 days, and every 7 days after 30 days to obtain seedling.

[0031] 2. Cultivation of complete seedlings:

[0032] Select the leaves of healthy low-day-o...

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Abstract

The invention relates to the technical field of asexual reproduction of seaweed artificial seedlings, and particularly relate to a seedling cultivation method for Sargassum vachellianum greville by using tissue culture. The method comprises acquiring fully grown wild Sargassum vachellianum greville with immature receptacles, holding culture, accelerating maturation, obtaining oosperms for static cultivation, and obtaining seedlings; segmenting the blades of seedlings of low age in days and the holdfasts of seedlings of low age in days for tissue culture, and obtaining regenerated seedlings; separating the regenerated seedlings from tissue blocks, culturing in vitro, obtaining complete seedlings, and holding culture; segmenting the blades of complete seedlings in holding culture again for tissue culture, and obtaining regenerated seedlings again; and finally separating the regenerated seedlings from tissue blocks, culturing in vitro, inducing the bases of the regenerated seedlings to regenerate holdfasts to form complete seedlings, and obtaining complete seedlings for another tissue culture. The blades and the holdfasts of the regenerated seedlings obtained by using the method of the invention can be used as regeneration sources for continuous use for many times, and are large in amount and easy for storage; the reproduction time is short, the acquisition of seedlings are not limited by objective conditions such as seasons, ecological conditions and the release amount of generative cells.

Description

technical field [0001] The invention belongs to the technical field of asexual propagation of seaweed artificial seedlings, and in particular relates to a method for raising seedlings by tissue culture of Sargassum waschii. Background technique [0002] Sargassum vachellianum Greville belongs to the genus Sargassum, brown-green in color, with fixator, main branch, side branch, leaf, air sac and other organs, sex is dioecious, perennial, and the peak breeding period is from June to July , is a warm temperate large brown algae concentrated in the coasts of Zhejiang, Fujian, Guangdong and Hong Kong in my country. Sargassum vacheronii itself is widely used in the fields of medicine, food, feed and organic fertilizer because it is rich in protein, carbohydrates, inorganic salts, vitamins, etc. Especially in the field of organic fertilizers, it has attracted people's attention. Compared with chemical fertilizers, Sargassum vacheronii fertilizer has natural advantages, no secondar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 严兴洪魏正蛟
Owner SHANGHAI OCEAN UNIV
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