Method for suppressing browning of carya cathayensis sarg explant
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A technology of explants and pecans, applied in the directions of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of uneven quality, long seedling period, hindering production and development, etc., and achieves inhibition of browning and operation. Simple and effective in inhibiting browning
Inactive Publication Date: 2013-04-03
ANHUI AGRICULTURAL UNIVERSITY +2
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[0004] At present, the propagation method used in hickory nut production mainly adopts seeding propagation, which has a long seedling period, uneven quality and easy degradation of seedlings, which seriously hinders the development of production; The increase of the content of carya-like substances increases, and a brown necrotic layer is formed on the injured part of the plant, which seriously hinders the survival rate of hickory grafting and cutting propagation; One of the main factors of culture, the research on inhibiting the browning of pecan explants is of great significance not only to its tissue culture, but also to its asexual reproduction
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Embodiment 1
[0024] The method for suppressing the browning of hickory nut explants comprises the steps:
[0025] ① Disinfection treatment
[0026] Take the spring hickory tree with axillary buds as explants, wash it with running water for 1-2 hours, soak it with carbendazim (1000 times the weight of liquid) for 30 minutes, put it on a super-clean workbench, and wash it with 75% alcohol (volume percentage) Soak for 90s, wash with sterile water for 3 times, then soak with 0.1% mercuric chloride (mercuric chloride) for 15min, wash with sterile water for 3 times.
[0027] ②, culture medium preparation
[0028] Based on 1L (total) medium (pH adjusted to 5.8-6.0), the medium contains:
[0036] In the culture medium, the catechins are 2g and 4g in turn, and the others are the same as in 1.
Embodiment 4
[0038] The additional components are: 4mg of 6-benzylaminopurine, 2.0mg of 6-furylmethyladenine, 0.05mg of 2,4-dichlorophenoxyacetic acid, 0.04mg of a-naphthaleneacetic acid, 8g of agar, and 25g of sucrose.
[0039] Others are with embodiment 1.
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Abstract
The invention relates to a forest tree seedling breeding technology and in particular relates to a method for suppressing browning of a carya cathayensis sarg explant. The method is characterized by selecting a carya cathayensis sarg stem with axillary buds as an explant in spring, disinfecting the explant, then inoculating the explant into a culture medium to which catechins and anti-browning agents are added, and firstly culturing a tissue culture vessel in the low temperature dark environment and then transferring the tissue culture vessel to the routine culture environment at the initial stage of inoculation. The method can conduce to obvious reduction of the browning rate of the carya cathayensis sarg explant and improvement of inductivity of the carya cathayensis sarg explant, is simple and convenient to operate, has a better browning suppression effect, and provides a technical support for tissue culture and asexual reproduction of carya cathayensis sarg.
Description
technical field [0001] The invention relates to forest tree seedling propagation technology, in particular to a method for inhibiting the browning of hickory nut explants. Background technique [0002] Hickory (Carya cathayensis Sarg) is a deciduous tree of the genus Hickory in the family Juglandaceae. It is concentrated in Ningguo, Jixi, Anhui, and Lin'an, Zhejiang, within the range of 29°30′-30°35′ north latitude and 118°45′-119°25′ east longitude. , Chun'an and other areas. [0003] Hickory is an important woody oil plant and dried fruit tree species in the southern region. The fruit is delicious in color and taste after frying, and the nuts are crispy and delicious, so it is loved by the public. The oil content of its dried kernels is 69.8-74.1%, and the protein content is 7.8-9.6%; it contains 8 kinds of fatty acids and 17 kinds of amino acids, of which 7 are necessary for the human body; It has extremely high nutritional value, and has the effect of lowering blood fa...
Claims
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