Defined medium for phalaenopsis tissue culture and culture method thereof

Abstract

The invention discloses a defined medium for phalaenopsis tissue culture and a culture method thereof. According to key points of the technical scheme, the defined medium comprises a pedicel inducingmedium and a rooting acclimation medium, wherein the pedicel inducing medium comprises the following raw materials: 250-350g of MS culture medium, 0.15-0.30 L of coconut water, 4-6mg of 6-benzyladenine, 4-6mg of adenine, 15-25g of sucrose and the balance of water, totaling 1L; a multiplication and strong seedling culture medium comprises the following raw materials: 2-4g of hyponex NO.1, 40-60g ofmashed banana, 10-30g of potato extract, 10-30g of onion, 10-20g of sucrose, 0.5-1.5g of activated carbon, 0.05-0.15g of citric acid and the balance of water, totaling 1L, wherein the pH value is regulated to 5.7-6.5 with a NaOH solution or a HCl solution; the rooting acclimation medium comprises the following raw materials: 1-3g of hyponex NO.1, 1-3g of hyponex NO.5, 40-60g of mashed banana, 140-60g of potato extract, 8-15g of fresh apple juice, 0.05-0.15g of citric acid, 16-22g of sucrose, 0.5-0.9g of activated carbon, and the balance of water, totaling 1L, wherein the pH value is regulatedto 5.7-6.0 with the NaOH solution or the HCl solution. The stability of the phalaenopsis in the tissue culture process is improved.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a combined culture medium for Phalaenopsis tissue culture and a culture method thereof. Background technique [0002] Phalaenopsis amabilis is a tropical aerial orchid, which is a perennial saprophytic herbaceous plant of the monocotyledon family Orchidaceae (Orchidaceae) Phalaenopsis (Phalaenopsis). It is one of the four most commercially valuable ornamental tropical orchids in the world, occupies an extremely important position in the world's flower industry, and has a huge market potential. [0003] At present, the rapid propagation methods of Phalaenopsis mainly include the use of explants such as roots, stems, and leaves to induce protocorms to induce meristems to achieve rapid propagation; directly induce clustered buds without callus. Using the method of tissue culture, the explants are collected and sterilized and inoculated on a suitable medium, and small buds with the same genet...

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Problems solved by technology

[0004] For example, the patent of CN103430842B with the authorized notification number discloses a method for rapid propagation of hybrid orchid tissue culture, which is a tissue culture method for directly inducing clustered buds through callus tissue, including disinfection of explants, induction of clustered buds, and formation of clustered buds. Proliferation, subculture, rooting culture, and transplanting of test-tube seedlings are six major steps. The four steps of induction of clustered buds, proliferation of clustered buds, subculture, and rooting culture all correspond to their respective culture media, that is, Said that in the connection of these four steps, the explants need to be taken out from the culture medium of the previous step and then transplanted into the culture medium of the next step, because the development of organs such as clustered shoots and roots of the plants is uneven during the tissue culture process. Immature and relatively fragile overall. During the inoculation process, it is easy to affect the growth of cluster buds, reduce the growth rate of plant cluster buds, and even improper operation will cause cluster buds to mutate or even die, which is not conducive to plant tissue culture.

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