A method for reducing the browning rate of leaf tissue culture of Begonia variegata

A technology for tissue culture and begonia, applied in the field of plant tissue culture, can solve the problems of limiting the tissue culture efficiency and browning of begonia variegata, and achieve the effects of reducing pollution rate and browning rate, eliminating browning, and increasing induction rate

Active Publication Date: 2022-06-07
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the tissue culture of Begonia iridatus, and there is a serious browning problem in conventional tissue culture, which seriously limits the tissue culture efficiency of Begonia iridatus

Method used

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  • A method for reducing the browning rate of leaf tissue culture of Begonia variegata
  • A method for reducing the browning rate of leaf tissue culture of Begonia variegata
  • A method for reducing the browning rate of leaf tissue culture of Begonia variegata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for reducing the browning rate of begonia iridescent leaf tissue culture, comprising the steps of:

[0026] Step (1), refrigerated preservation treatment: refrigerated fresh young leaves of Begonia variegata in a refrigerator at 4°C for 1 day;

[0027] Step (2), disinfection and treatment of explants: After washing the young leaves treated in step (1) with washing powder water, soak them in alcohol with a volume concentration of 75% for 30 seconds, and then wash them with 0.1% HgCl 2 The solution was treated for 5 minutes, followed by rinsing with sterile water for 3 times; then under aseptic conditions, the water was absorbed with sterile filter paper, and then the leaves were cut into 2cm 2 size leaf disk;

[0028] Step (3), dark culture: scratch the back of the leaf disc obtained in step (2), inoculate the primary culture medium with the back of the leaves facing down, and culture in the dark for 5 days at a culture temperature of 23±2°C;

[0029] Step (4)...

Embodiment 2

[0034] A method for reducing the browning rate of begonia iridescent leaf tissue culture, comprising the steps of:

[0035] Step (1), refrigerated preservation treatment: refrigerated fresh young leaves of Begonia variegata in a refrigerator at 4°C for 2 days;

[0036] Step (2), disinfection and treatment of explants: After washing the young leaves treated in step (1) with washing powder water, soak them in alcohol with a volume concentration of 75% for 30 seconds, and then wash them with 0.1% HgCl 2 The solution was treated for 8 minutes, and then rinsed with sterile water for 3 times, each time for 2 minutes; then under sterile conditions, use sterile filter paper to absorb water, and then cut the leaves into 2cm 2 size leaf disk;

[0037] Step (3), dark culture: scratch the back of the leaf disc obtained in step (2), inoculate the primary culture medium with the back of the leaves facing down, and culture in the dark for 5 days at a culture temperature of 23±2°C;

[0038]...

Embodiment 3

[0043] A method for reducing the browning rate of begonia iridescent leaf tissue culture, comprising the steps of:

[0044] Step (1), refrigerated preservation treatment: refrigerated fresh young leaves of Begonia variegata in a refrigerator at 4°C for 1 day;

[0045] Step (2), disinfection and treatment of explants: After washing the young leaves treated in step (1) with washing powder water, soak them in alcohol with a volume concentration of 70% for 20 seconds, and then wash them with 0.1% HgCl 2 The solution was treated for 5 minutes, then rinsed with sterile water for 3 times, each time for 2 minutes; then under sterile conditions, use sterile filter paper to absorb water, and then cut the leaves into 1.8cm 2 size leaf disk;

[0046] Step (3), dark culture: scratch the back of the leaf disc obtained in step (2), inoculate the primary culture medium with the back of the leaves facing down, and culture in the dark for 5 days at a culture temperature of 23±2°C;

[0047] St...

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Abstract

The invention relates to a method for reducing the browning rate of begonia leaf tissue culture and belongs to the technical field of plant tissue culture. In this method, the young leaves of fresh Begonia variegata are refrigerated before inoculation, and after being sterilized by alcohol and mercuric chloride, different light culture methods in tissue culture are combined with continuous bottle transfer, that is, first dark culture→semi-dark culture→ Light culture, and transfer once every 5-7 days, so as to effectively prevent or even eliminate the browning of Begonia variegata, reduce the browning rate and increase its induction rate to a certain extent, and promote the growth of plants. Compared with the existing method for reducing browning, the method of the invention does not need to add special equipment, has low concentration, low cost, simple operation and easy popularization and application.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for reducing the browning rate of leaf tissue culture of begonia iridatus. Background technique [0002] Colored Begonia ( Begonia masoniana ) is a perennial evergreen herb belonging to the family Begoniaceae. It has oval leaves with wrinkles and bristles on the surface. There is a reddish-brown horseshoe-shaped ring in the center of the yellow-green leaves. It is very beautiful and is a relatively rare species of begonias. However, as a species of Begonia, its seeds are not easy to obtain, its sexual reproduction ability is weak, and its offspring are highly variable, so it is difficult to maintain its excellent traits. As one of the contemporary emerging biotechnology methods, plant tissue culture technology has many advantages such as high reproduction coefficient, stable genetic traits, and no seasonal restrictions, so it is very suitable fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005Y02A40/22
Inventor 段青王继华杜文文马璐琳王祥宁崔光芬贾文杰
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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