Culture method of salt-tolerant anaerobic ammonium oxidation bacteria

A technology of anaerobic ammox bacteria and cultivation methods, applied in chemical instruments and methods, bacteria, water/sludge/sewage treatment, etc., can solve problems such as not being able to meet needs, achieve good economic and environmental benefits, and be easy to cultivate , good biological activity

Active Publication Date: 2013-03-06
CHINA PETROLEUM & CHEM CORP +1
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0008] The dilution method used in the prior art to treat high-salinity ammonia-nitrogen wastewater can no longer meet the needs, and how to cultivate anammox bacteria suitable for high-salinity ammonia-nitrogen wastewater has become a technical problem

Method used

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  • Culture method of salt-tolerant anaerobic ammonium oxidation bacteria
  • Culture method of salt-tolerant anaerobic ammonium oxidation bacteria

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Experimental program
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Embodiment 1

[0027] Step A, enrichment and cultivation of anammox bacteria group: the activated sludge taken from the anaerobic tank of the sewage treatment workshop of a chemical plant is used as the bacterial species, and the activated sludge is inoculated to the figure 1 In the biological fermentation tank shown, the composition of the simulated wastewater in the biological fermentation tank is: NH 4 + -N(NH 4 HCO 3 )100~200mg / L; NO 2 - -N(NaNO 2 )100~250mg / L; Fe 2+ (FeSO 4 ·7H 2 O) 12mg / L; Mg 2+ (MgSO 4 ·7H 2 O) 20mg / L; P (KH 2 PO 4 )65mg / L; Add sodium bicarbonate according to the carbon-nitrogen ratio (0.5-20):1. The dissolved oxygen value in the fermenter is controlled at 0-0.5mg / L, the pH is controlled at 7.5-8.5, and the temperature is controlled at 25-38°C. Each operation cycle of the biological fermentation tank is 48 hours, including 30 minutes of water intake, 44 hours of anaerobic reaction, 2 hours of sedimentation, and 1 hour of drainage, and the rest of the tim...

Embodiment 2

[0030] Step A, enrichment culture of anammox bacteria group: the activated sludge taken from the facultative tank test device of a sewage treatment plant is used as the bacterial species, and the activated sludge is inoculated to the figure 1 In the biological fermentation tank shown, the composition of the simulated wastewater in the biological fermentation tank is: NH 4 + -N(NH 4 HCO 3 )50~150mg / L; NO 2 - -N(NaNO 2 )50~200mg / L; Fe 2+ (FeSO 4 ·7H 2 O) 10mg / L; Mg 2+ (MgSO 4 ·7H 2 O) 15mg / L; P (KH 2 PO 4 ) 60mg / L; Add sodium bicarbonate according to the carbon-nitrogen ratio (0.5-20):1. The dissolved oxygen value in the fermenter is controlled at 0-0.5mg / L, the pH is controlled at 7.0-8.5, and the temperature is controlled at 25-38°C. Each operation cycle of the biological fermentation tank is 24 hours, including 30 minutes of water inflow, 21 hours of anaerobic reaction, 1.4 hours of sedimentation, and 1 hour of drainage, and the rest of the time is idle period. ...

Embodiment 3

[0033] In this embodiment, the process of enriching and culturing the anammox bacteria group in Step A is the same as that in Embodiment 1.

[0034] Step B, domestication and cultivation of salt-tolerant anammox bacteria: after the enrichment and cultivation of anammox bacteria, enter the process of domestication and cultivation of salt-tolerant anammox bacteria. In the initial stage of acclimation, sodium chloride and sodium sulfate in the simulated wastewater were added at 1.5g / L and 6.5g / L respectively; / L concentration gradient increases, after three levels of salinity acclimatization culture, the final salt concentration is 6.5g / L and 13.5g / L respectively. The domestication cycle of each salinity is 20 days; each operation cycle in the biological fermentation tank is 48 hours, including water inflow for 30 minutes, anaerobic reaction for 44 hours, precipitation for 2 hours, drainage for 1 hour, and the rest of the time is idle period. After acclimatization, the fungus ge...

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Abstract

The invention provides a culture method of salt-tolerant anaerobic ammonium oxidation bacteria, which comprises the following steps of: A, enrichment culture: performing enrichment culture on the strain to obtain anaerobic ammonium oxidation bacteria; and B, bacteria acclimation culture: gradually increasing the concentration of salt in the culture solution to perform salt tolerance acclimation culture of the anaerobic ammonium oxidation bacteria subjected to the enrichment culture in the step A, wherein the acclimation period of the bacterial at each salt concentration is 15-30 days; and the step A and step B both adopt sequencing batch culture, and the dissolved oxygen value in the culture solution is 0-0.5mg/L. The bacteria cultured by the method provided by the invention are applied to high-salt ammonia nitrogen wastewater, and the total nitrogen removal rate is high. By adopting the method provided by the invention to culture the salt-tolerant anaerobic ammonium oxidation bacteria, the range of available substrates is wide, and the culture is easy to implement; and additional organic carbon source is not required in the bacteria culture and application process, investment is greatly saved, and the wastewater treatment cost is reduced. The bacteria culture method provided by the invention can be applied to large-scale production, and has good economic and environmental benefits.

Description

technical field [0001] The present invention relates to a method for cultivating salt-tolerant anammox bacteria, more specifically, to a method for enriching and cultivating anammox bacteria with ammonia nitrogen in high-salt wastewater, which belongs to microbial technology Specifically, it can be applied to the treatment of high-salt ammonia-nitrogen industrial wastewater such as catalyst industry and nitrogen fertilizer industry. Background technique [0002] Ammonia nitrogen is an important factor in water pollution. Excessive ammonia nitrogen in water can easily cause excessive reproduction of plants and algae in water, weaken the self-purification ability of water body, deteriorate water quality and cause eutrophication of water body. In the prior art, the main treatment methods for ammonia nitrogen wastewater include physical and chemical methods, advanced oxidation methods and biological methods. [0003] Among them, physical and chemical methods include breakpoint ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34
Inventor 王道泉孙杰马兰兰赵璞赵桂瑜魏令勇
Owner CHINA PETROLEUM & CHEM CORP
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