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Large-scaled culture method of high-concentration nitrosobacteria and application thereof

A technology for nitrosating bacteria and a culturing method is applied in the field of large-scale cultivation of high-concentration nitrosifying bacteria, which can solve problems such as being unsuitable for large-scale cultivation and affecting the growth of nitrifying bacteria, achieve significant energy-saving benefits, and improve ammonia nitrogen removal efficiency and activity. Good results

Active Publication Date: 2010-05-19
ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although it can handle high-concentration ammonia-nitrogen wastewater, if no organic substances are added in the actual cultivation, the activated sludge may be degraded. If organic substances are added, the growth of nitrifying bacteria will be affected, so it is not suitable for large-scale cultivation.

Method used

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  • Large-scaled culture method of high-concentration nitrosobacteria and application thereof
  • Large-scaled culture method of high-concentration nitrosobacteria and application thereof
  • Large-scaled culture method of high-concentration nitrosobacteria and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The directional enrichment culture of embodiment 1 nitrosative bacteria (1)

[0023] The enrichment culture of experimental nitrosative bacteria uses a 10L glass tank simple culture device, equipped with a small stirring device, and the aeration head is passed into the bottom of the tank to provide oxygen. Add the enrichment culture solution with an initial ammonia nitrogen mass concentration of 100mg / L, take the activated sludge with a volume ratio of 20% from the sewage treatment plant and inoculate once, control the enrichment culture temperature at 29°C, pH value at 8.1, and dissolved oxygen at 3.5mg / L L, the stirring speed is 120r / min. The composition of the enriched medium is 1.0g NH per liter of water 4 HCO 3 , 0.5g KH 2 PO 4 , 1.0g Na 2 HPO 4 , 0.4g MgSO 4 ·7H 2 O, 1.0g NaHCO 3 and 0.5g CaCO 3 . Add 1g / L NH in mid-culture 4 HCO 3 And growth promoter 0.5g / L KH 2 PO 4 , 1g / L Na 2 HPO 4 , 0.5g / L MgSO 4 ·7H 2 O, 0.1g / L FeSO 4 ·7H 2 O, to be cult...

Embodiment 2

[0024] The directional enrichment culture of embodiment 2 nitrosative bacteria (two)

[0025] The enrichment culture of experimental nitrosative bacteria uses a 10L glass tank simple culture device, equipped with a small stirring device, and the aeration head is passed into the bottom of the tank to provide oxygen. Add the enrichment culture solution with an initial ammonia nitrogen mass concentration of 30mg / L, take the activated sludge with a volume ratio of 0.5% from the sewage treatment plant for one inoculation, control the enrichment culture temperature at 27°C, pH value at 7.5, and dissolved oxygen at 1.5mg / L L, the stirring speed is 50r / min. The composition of the enriched medium is 0.1g NH per liter of water 4 Cl, 0.1g K 2 HPO 4 , 0.1g NaH 2 PO 4 , 0.05g MgSO 4 ·7H 2 O, 0.1g NaHCO 3 and 0.1g CaCO 3 . Add NH in the middle 4 Cl 0.5g / L and growth promoter 0.05g / LK 2 HPO 4 , 0.5g / L NaH 2 PO 4 , 0.1g / L MgSO 4 ·7H 2 O, 0.01g / L FeSO 4 ·7H 2 O, to be cultur...

Embodiment 3

[0026] Directional enrichment culture of embodiment 3 nitrosative bacteria (three)

[0027] The enrichment culture of experimental nitrosative bacteria uses a 10L glass tank simple culture device, equipped with a small stirring device, and the aeration head is passed into the bottom of the tank to provide oxygen. Add the enrichment culture solution with an initial ammonia nitrogen mass concentration of 200mg / L, take the activated sludge with a volume ratio of 20% from the sewage treatment plant and inoculate once, control the enrichment culture temperature at 32°C, pH value at 8.0, and dissolved oxygen at 3.5mg / L L, the stirring speed is 100r / min. The composition of the enriched culture solution is 1g (NH 4 ) 2 SO 4 , 2g K 2 HPO 4 , 1g NaH 2 PO 4 , 0.5g MgSO 4 ·7H 2 O, 2g NaHCO 3 and 2g CaCO 3 . Addition in the middle (NH 4 ) 2 SO 4 1g / L and growth promoter 1.0g / LK 2 HPO 4 , 1g / L NaH 2 PO 4 , 1g / L MgSO 4 ·7H 2 O, 0.5g / L FeSO 4 ·7H 2 O, to be cultured fo...

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Abstract

The invention discloses a large-scaled culture method of high-concentration nitrosobacteria and application thereof. The method comprises the following steps of: (1) adding an enrichment culture solution with initial ammonia nitrogen mass concentration of 30-500mg / L in a reaction device with functions of stirring, heating and aeration; preparing activated sludge of a waste water treatment plant in activated sludge of 0.5-20% for primary inoculation to conduct the enrichment culture of the nitrosobacteria by 2-6d as a culture cycle, wherein extra ammonium salts and a growth promoter are added during the enrichment culture of the nitrosobacteria; (2) ending the culture when the concentration of NO2-N in the solution is accumulated to reach 70-95% of the concentration of total nitrogen; adding a flocculating agent for flocculation and sedimentation; and allowing to stand still to remove supernate, and then adding the enrichment culture solution to start the culture of the second cycle; and (3) continuously conducting 3-8 cycles of the enrichment culture to obtain the high-concentration nitrosobacteria. By adopting the nitrosobacteria to strengthen the bi-nitrification treatment of ammonia nitrogen contained waste water, the method has the advantages of high stress resistance, low cost and high efficiency and is suitable for the complicated treatment of ammonia nitrogen contained industrial waste water.

Description

technical field [0001] The invention relates to the field of environmental biotechnology, in particular to a method for large-scale cultivation of high-concentration nitrosative bacteria and its application. Background technique [0002] Ammonia nitrogen pollution can cause excessive reproduction of aquatic plants and algae, deteriorating water quality, and is an important factor causing eutrophication of water bodies. The biological nitrification reaction with nitrosating bacteria as the core is to use nitrifying bacteria to convert NH 4 + The process of converting into nitrite and nitrate is an important link in water purification. In recent years, the biological denitrification process using nitrifying bacteria to treat ammonia nitrogen wastewater, such as short-cut nitrification and denitrification and simultaneous nitrification and denitrification, has been favored by people, but the research only stays in the laboratory stage. The nitrification of nitrosifying bacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34
Inventor 徐灏龙李明智仝武刚白俊跃章一丹王长智胡智锋张刚
Owner ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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