Enrichment culture method of salt-tolerant nitrifying bacterium communities

A technology of enrichment culture and nitrifying bacteria, applied in the field of microorganisms, can solve the problems of unsuitable treatment of high-salt ammonia nitrogen wastewater, achieve good economic and environmental benefits, easy to cultivate, and reduce the cost of wastewater treatment

Inactive Publication Date: 2012-03-21
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

[0008] The invention provides a method for enriching and cultivating salt-tolerant nitrifying bacteri...

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  • Enrichment culture method of salt-tolerant nitrifying bacterium communities

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Embodiment 1

[0048] a. Enrichment culture of nitrifying bacteria: activated sludge is taken from a nitrification test device of a sewage treatment plant, and the activated sludge is inoculated into the SBR reactor at a concentration of 2g / L, and enriched culture medium is added, and the composition of the enriched culture medium is : NH 4 + -N(NH 4 HCO 3 )200~600mg / L; Fe 2+ (FeSO 4 ·7H 2 O) 10mg / L; Mg 2+ (MgSO 4 ·7H 2 O) 15mg / L; P (KH 2 PO 4 ) 60mg / L; Add sodium bicarbonate according to the carbon-nitrogen ratio (0.5-20):1. During the enrichment and cultivation of nitrifying bacteria, one operating cycle is 24 hours, of which: water intake for 15 minutes, aeration for 20 hours, sedimentation for 2 hours, drainage for 1 hour, and the remaining time is the idle period; the dissolved oxygen value in the reactor is controlled at 2.5-4.5mg / L, the pH is controlled between 6.0 and 8.5, and the temperature is controlled between 15 and 37°C. In the process of enriching and cultivating ...

Embodiment 2

[0051] a. Enrichment culture of nitrifying bacteria: the activated sludge is taken from the aeration tank of the sewage treatment workshop of a chemical plant, and the activated sludge is inoculated into the SBR reactor at a concentration of 2.5g / L, and the enrichment culture solution is added for enrichment culture The liquid composition is: NH 4 + -N(NH 4 HCO 3 )100~700mg / L; Fe 2+ (FeSO 4 ·7H 2 O) 12mg / L; Mg 2+ (MgSO 4 ·7H 2 O) 20mg / L; P (KH 2 PO 4 )65mg / L; Add sodium bicarbonate according to the carbon-nitrogen ratio (0.5-20):1. During the enrichment and cultivation of nitrifying bacteria, an operation cycle is 24 hours, in which: 15 minutes of water inflow, 20 hours of aeration, 2 hours of sedimentation, 1 hour of drainage, and the rest of the idle period; the dissolved oxygen value in the reactor is controlled at 1.5-5.5 mg / L, the pH is controlled between 6.5 and 8.8, and the temperature is controlled between 15 and 37°C. In the process of enriching and culti...

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Abstract

The invention discloses an enrichment culture method of salt-tolerant nitrifying bacterium communities, which comprises the steps that: activated sludge is inoculated, and the culture mode is the sequencing batch type enrichment culture; the enrichment culture is carried out in simulated wastewater, the main ingredient of nutritive salt of the enrichment culture is inorganic salt, the substrate is externally added ammonium salt, and a carbon source is inorganic carbonate; the pH value in an aeration tank in the enrichment culture process is regulated and controlled through sodium carbonate and sodium bicarbonate, and the dissolved oxygen value is regulated through the aeration quantity; a method for gradually increasing the salinity is adopted for domesticating the nitrifying bacterium communities; and when no filamentous bacterium is observed in the sludge and the number and the variety of protozoans are few, the enrichment culture of the nitrifying bacterium communities is finished. The enrichment culture method has the advantages that the available matrix range is wide, the culture is easy, the concentration of the cultured salt-tolerant nitrifying bacterium communities is high, the activity is good, the biochemical treatment removal rate of high-salt ammonia-nitrogen wastewater can be obviously improved, the enrichment culture method is suitable for the treatment of various ammonia-nitrogen industrial wastewater and is suitable for the scale production, the wastewater treatment cost is reduced, and good economic benefits and environmental benefits are realized.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a method for enriching and culturing salt-tolerant nitrifying bacteria. Background technique [0002] Ammonia nitrogen is an important factor in water pollution. Excessive ammonia nitrogen in water can easily cause excessive reproduction of plants and algae in water, weaken the self-purification ability of water body, deteriorate water quality and cause eutrophication of water body. In the current prior art, the main treatment methods of ammonia nitrogen wastewater include physicochemical method, advanced oxidation method and biological method. [0003] Physicochemical methods include breakpoint chlorination, chemical precipitation, ion exchange, stripping / stripping, membrane technology, electrodialysis and crystallization. The physicochemical method has disadvantages such as harsh reaction conditions and high operating costs, and its application is limited to some indust...

Claims

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Application Information

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IPC IPC(8): C12N1/00C02F3/34
Inventor 王道泉马兰兰赵璞赵桂瑜
Owner CHINA PETROLEUM & CHEM CORP
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