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Immobilized microbial preparation and method of treating meat product processing waste water by utilizing same

A technology for immobilizing microorganisms and processing wastewater, applied in biological water/sewage treatment, chemical instruments and methods, fixed on/in organic carriers, etc. High concentration of pollutants and other problems, to achieve high-purity and high-efficiency strains, strong ability to adapt to water quality and pH changes, and high treatment efficiency

Active Publication Date: 2013-07-17
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with general industrial wastewater and domestic wastewater, the production wastewater of the meat processing industry has the characteristics of high pollutant concentration and large wastewater discharge per unit product. It is usually difficult to achieve ideal treatment effects by using ordinary wastewater treatment processes
If it is not treated in time, it will cause serious environmental pollution, which is not only harmful to the health of humans and animals, but also causes secondary pollution in the production process of meat products, affecting the quality of products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] An immobilized microbial preparation prepared by the following steps:

[0023] S1. Fermentation culture of strains, inoculate Bacillus subtilis (Bacillus subtilis ATCC 14579), Bacillus megaterium ATCC 13578, Nitrobacter ATCC 14123 and Nitrosomonas europaea ATCC 25978, respectively Into the enrichment medium, cultured at 30°C and 160rpm for 24h, centrifuged at 5000rpm for 10min to obtain the logarithmic growth phase cells of each bacteria;

[0024] S2, the mixing of thalline, after the cells in step S1 are washed 2 times with phosphate buffer saline, by volume percentage, get 40% Bacillus subtilis, 30% Bacillus megaterium, 15% Nitrobacter, 15% subtilis Nitrobacteria are mixed and suspended in physiological saline to obtain a bacterial cell mixture;

[0025] The immobilization of S3, PVA-sodium alginate, it comprises the following substeps:

[0026] S31. Preparation of PVA gel: Take PVA and add it to water, dissolve it completely at 80°C, the weight / volume ratio of PVA ...

Embodiment 2

[0033] Immobilized microbial preparations, immobilized microbial preparations, are prepared by the following steps:

[0034] S1. Fermentation culture of strains, inoculate Bacillus subtilis (Bacillus subtilis ATCC 14579), Bacillus megaterium ATCC 13578, Nitrobacter ATCC 14123 and Nitrosomonas europaea ATCC 25978, respectively In the enrichment medium, cultured at 28°C and 170rpm for 26h, centrifuged at 5000rpm for 10min to obtain the logarithmic growth phase cells of each bacteria;

[0035] S2, the mixing of thalli, after the cells in step S1 are washed 3 times with phosphate buffer saline, by volume percentage, get 60% Bacillus subtilis, 25% Bacillus megaterium, 10% Nitrobacter, 5% subtilis Nitrobacteria are mixed and suspended in physiological saline to obtain a bacterial cell mixture;

[0036] The immobilization of S3, PVA-sodium alginate, it comprises the following substeps:

[0037] S31. Preparation of PVA gel: Take PVA and add it to water, dissolve it completely at 80°...

Embodiment 3

[0044] Immobilized microbial preparations, immobilized microbial preparations, are prepared by the following steps:

[0045] S1. Fermentation culture of strains, inoculate Bacillus subtilis (Bacillus subtilis ATCC 14579), Bacillus megaterium ATCC 13578, Nitrobacter ATCC 14123 and Nitrosomonas europaea ATCC 25978, respectively Into the enrichment medium, cultured at 32°C and 150rpm for 22h, centrifuged at 5000rpm for 10min to obtain the logarithmic growth phase cells of each bacteria;

[0046] S2, the mixing of thalline, after the cells in step S1 are washed 2 times with phosphate buffer saline, by volume percentage, get respectively 55% Bacillus subtilis, 20% Bacillus megaterium, 12% Nitrobacter, 13% subtilis Nitrobacteria are mixed and suspended in physiological saline to obtain a bacterial cell mixture;

[0047] The immobilization of S3, PVA-sodium alginate, it comprises the following substeps:

[0048] S31. Preparation of PVA gel: Take PVA and add it to water, dissolve it...

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PUM

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Abstract

The invention discloses an immobilized microbial preparation which is prepared through the following steps: S1, fermentation culturing strain: respectively inoculating bacillus subtilis, bacillus megatherium, nitrobacter and nitrosomonas in an enrichment medium to be cultured; S2, mixing thallus to obtain mixed thallus liquor; S3, immobilizing PVA-sodium alginate, namely uniformly mixing PVA gel with the mixed thallus liquor, dripping the mixture into a boric acid solution containing CaCl2 while continuously stirring at a low speed, immobilizing and crosslinking the mixture, taking out the mixture, washing the mixture through deionized water, and freeze drying the mixture to obtain the immobilized microbial preparation. The invention further discloses a method of treating meat product processing waste water by utilizing the immobilized microbial preparation. The immobilized microbial preparation disclosed by the invention has the beneficial effects that the microorganism categories are various, various pollutants in the waste water can be degraded, the degrading speed is fast and the capability is strong; the immobilized microbial preparation is suitable for changes of water quality and pH; the sludge yield is small, the products are liable to separate, the stability is good, the technique operation is simple, and the production cost is low.

Description

technical field [0001] The invention relates to the technical field of meat product processing wastewater treatment technology, in particular to an immobilized microbial preparation and a method for treating meat product processing wastewater by using the preparation. Background technique [0002] In recent years, with the development of my country's social economy and the continuous improvement of people's living standards, people's consumption of meat products is also increasing. At the same time, the meat processing industry has also developed rapidly. However, with the rapid development of the meat processing industry, the pollution problems in this industry, especially the water pollution problems, have attracted more and more attention. Compared with general industrial wastewater and domestic wastewater, production wastewater in the meat processing industry has the characteristics of high pollutant concentration and large wastewater discharge per unit product. It is us...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C02F3/34C02F103/32
Inventor 李明元龙吉海马力向文良饶瑜张庆常伟周巍熹张友华胡怀容唐萍鲜欣言
Owner XIHUA UNIV
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