Method for treating deep-sea spilled oils by using barophilic bacteria

Abstract

The invention provides a method for treating deep-sea spilled oils by using barophilic bacteria. The method particularly comprises that a pressure unit with the internal pressure of 0.3-0.4 MPa is adopted for gradient screening and domestication of the petroleum-degrading barophilic baceria, and with porous zirconium oxide as a barophilic bacteria carrier, degradation effects of crude oils with different concentrations are tested. Aiming at environments of deep-sea high pressure, complex ocean current and the like, indigenous bacteria in sea oil spillage region bottom mud is used as a bacterial source, a special-made pressurization device is utilized, and the gradient-concentration crude oils are used as a sole carbon source, the indigenous bacteria are subjected to domestication and screening, and thus the barophilic bacteria which can degrade the crude oils under the pressure of 0.3-0.4 MPa are obtained. The screened barophilic bacteria are used as a study object, the crude oils under the pressure of 0.3-0.4 MPa are used as the sole carbon source, the degradation experiments with the crude oil concentrations of 1 g / L, 3 g / L and 5 g / L respectively are carried out, determination is continuously carried out for 30 days, and the degradation rates reach 86.9%, 68.3% and 49.1% respectively; therefore, the screened and domestication barophilic bacteria have relatively good degradation efficiency on the crude oils under the high pressure of 0.3-0.4 MPa, and a new way is provided for bioremediation of the deep-sea spilled oils.

Description

technical field [0001] The invention belongs to the technical field of deep-sea oil spill treatment, and in particular relates to a screening, bacteria-carrying and restoration method for treating deep-sea oil spill barophilic bacteria. Background technique [0002] With the world's increasing demand for oil and its products, oil spill accidents in the process of offshore mining, transportation, loading and unloading, and utilization of oil are also increasing, causing serious environmental pollution. Taking Bohai Bay as an example, the Bohai Sea is the only semi-enclosed inland sea in my country, covering an area of ​​77,284 square kilometers. It has unique resource and geographical advantages, and is an important support system for the Bohai Rim Economic Circle. The Bohai Rim region includes Shandong, Hebei, Liaoning and Tianjin provinces and one city. Over the past ten years, the marine economy in the Bohai Rim region has maintained a high-speed growth rate of more than ...

AI Technical Summary

Problems solved by technology

And the notification number is CN102604924, and the name is "a kind of marine oil degrading bacterial agent and its preparation" and it is described that the bacterial agent is attached to a kind of buoyant biological carrier. Good degradation effect, but it is not suitable for the degradation of crude oil in deep sea sediments

Images