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Preparation method of microbial preparation capable of enabling crop straws to be rotten

A microbial preparation and crop straw technology, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of improper dosage of chemical additives, affecting the survival rate of microorganisms, complicated preparation of rotten agent, etc., achieving good results and easy large-scale Area popularization and application, beneficial to the effect of environmental protection

Inactive Publication Date: 2017-10-31
刘从荡
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of this kind of rotten agent is more complicated. Firstly, the various microorganisms contained in it should be cultured separately so as not to contaminate each other.
During the synthesis process, if the operation is not careful enough, it will cause an explosion, endangering personal safety, and the improper amount of chemical additives used will also affect the survival rate of microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. A microbial agent that rots crop straw.

[0034] (1) WH1 strain: 5% (2) WH2 strain: 35%

[0035] (3) WH3 strain: 10% (4) WH4 strain: 15%

[0036] (5) WH5 strain: 35%.

[0037] 2. The preparation method of microbial preparation - rotten stalk agent:

[0038] (1) Preparation of WH strains: Mix WH1 strains, WH2 strains, WH3 strains, WH4 strains, and WH5 strains according to the above ratio to form a WH strain.

[0039] (2) Secondary strain culture:

[0040] The secondary bacterial culture is mixed and mixed thoroughly to prepare (percentage by weight) according to the following formula:

[0041] Rice Bran 80% Soybean Flour 16% Milk Powder 2% Meat and Bone Meal 0.1%

[0042] Fish bone meal 0.1% sugar 0.8% water 1%.

[0043] The prepared secondary strain culture medium was inoculated with WH flora at room temperature, the culture temperature was 35°C, the pH value was 7.0, and the culture time was 9 days to obtain the secondary active furfur of WH flora.

[0044] (...

Embodiment 2

[0052] This embodiment differs from example 1 in that:

[0053] 1. The ratio of WH flora is (percentage by weight):

[0054] (1) WH1 strain: 12% (2) WH2 strain: 27% (3) WH3 strain: 20%

[0055] (4) WH4 strain: 11% (5) WH5 strain: 30%

[0056] 2. Secondary strain culture conditions: culture temperature is 37°C, culture time is 8 days.

[0057] 3. Fermentation conditions of WH flora: the filter mud was inoculated with WH flora at 35° C., and the inoculum amount was 0.1%. The temperature of the inoculated and fermented filter mud gradually rises to 62°C, the pH value is 7.0, and it matures after 10 days of fermentation.

[0058] All the other parameters and process steps are the same as Example 1.

[0059] Finally, 85% of the base material and 15% of the WH flora secondary active Pityriasis fungus (by weight percentage) were mixed, pulverized, and packaged to make a rotten agent.

Embodiment 3

[0061] This embodiment differs from Example 1 in that:

[0062] 1. The ratio of WH flora is (percentage by weight):

[0063](1) WH1 strain: 20% (2) WH2 strain: 17% (3) WH3 strain: 30%

[0064] (4) WH4 strain: 8% (5) WG5 strain: 25%

[0065] 2. Secondary strain culture conditions: culture temperature is 40°C, culture time is 7 days.

[0066] 3. Fermentation conditions of WH flora: Inoculate the filter mud with WH flora at 50°C, the inoculum amount is 0.01%, the temperature of the inoculated and fermented filter mud gradually rises to 65°C, the pH value is 7.2, and it matures after 7 days of fermentation.

[0067] All the other parameters and process steps are the same as Example 1.

[0068] Finally, 80% of the base material and 20% of the WH flora secondary active furfur (percentage by weight) are mixed, pulverized, and packaged to make a rotten agent.

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Abstract

The invention relates to a method for preparing a microbial preparation for rotting crop straw. The microbial flora used is named WH flora, which belongs to the genus Bacillus. Filter mud is used as the three-stage medium and carrier of WH flora. Group preparation, secondary strain cultivation, tertiary strain cultivation and other process steps are used to make rotten agent. The degradation products of WH flora provide energy and nutrition to native cellulolytic bacteria and various spoilage flora, enabling them to rapidly activate and cooperate with WH flora to rapidly decay crop straws. The method has the advantages of simple process, low cost, high number of live bacteria, easy use and easy popularization and application in a large area.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to a method for preparing a microbial preparation for decomposing plant stalks. Background technique [0002] my country produces hundreds of millions of tons of crop stalks every year. Except for a small amount that is directly used as feed, a large amount of remaining crop stalks are burned and pollute the environment. For many years, scientists have devoted themselves to using microorganisms to return crop stalks to the fields. At present, there is a kind of microorganism preparation—stalk rotting agent synthesized with several other high-efficiency microorganisms based on Sporosporium thermophilicum. The preparation of this kind of rotten agent is relatively complicated. Firstly, the various microorganisms contained in it must be cultured separately so as not to contaminate each other. During the synthesis process, if the operation is not careful enough, it will cause an explosion, e...

Claims

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Application Information

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IPC IPC(8): C12N1/00C05F11/08
CPCC12N1/00C05C9/00C05F11/08
Inventor 刘从荡
Owner 刘从荡
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