Microbial composite repairing liquid organic fertilizer and preparation method thereof

Abstract

The invention provides a microbial composite remediation liquid organic fertilizer and a preparation method thereof, and aims to solve the problems of single function, limited pollution abatement capability and the like of a traditional liquid fertilizer. Through synergism of strains of bacillus velezensis, bacillus methylotrophicus, pseudomonas putida and methanogenic archaea, recycling of agricultural wastes such as straw and mushroom dreg compost and decomposition, extraction of humic acid through enzymolysis and a formula of green additives (mineral source potassium fulvate, sophorolipid and the like), chemical passivation of heavy metals, pesticide degradation and soil improvement are realized; and dynamic fermentation control and membrane separation processes are combined, so that the method has the characteristics of efficient repair and low-carbon sustainability, and an integrated solution is provided for pollution treatment of complex farmlands.

Description

Technical Field

[0001] This patent falls under the technical field of agricultural organic fertilizer technology, specifically relating to a liquid organic fertilizer prepared using microbial composite remediation technology and its preparation method. Its core lies in achieving multiple functional integrations through the synergistic effect of multiple microbial strains, resource utilization of agricultural waste, and green additive formulation, including heavy metal pollution passivation, pesticide residue degradation, soil quality improvement, and crop growth promotion and stress resistance. It belongs to the field of environmentally friendly fertilizer innovation within modern agricultural sustainable development technology. Background Technology

[0002] In the process of agricultural modernization, liquid organic fertilizers, as an important type of fertilizer, play a crucial role in improving soil fertility and promoting crop growth. However, traditional liquid organic fertilizers face many challenges in their production and application.

[0003] On the one hand, the pollution problems caused by pesticides and heavy metals widely used in agricultural production are becoming increasingly serious. These pollutants remain in the soil and fertilizers, not only affecting the soil ecological balance but also harming human health through the food chain. Traditional fertilizer treatment technologies mostly focus on the removal of single pollutants. For example, patent CN110498719B discloses a microbial fertilizer that uses Bacillus mucilaginosus and potassium humate to synergistically degrade pesticides, but its passivation ability for heavy metals is limited. Patent CN118084562A utilizes the complexation of sodium silicate and fly ash to passivate heavy metals, but it does not involve pesticide degradation. Existing technologies lack a composite remediation solution that simultaneously addresses heavy metal passivation and pesticide degradation, making it difficult to meet the demands of modern agriculture for environmentally friendly fertilizers.

[0004] On the other hand, declining soil quality and insufficient crop resistance are also significant factors hindering sustainable agricultural development. Patent CN108840751A proposes enhancing crop resistance through amino oligosaccharides and betaine, but it does not incorporate soil-improving functions. While microbial fertilizers in patent CN110498719B can improve soil structure, their ability to withstand extreme conditions is insufficient. Traditional fertilizers have limited functions and struggle to simultaneously address multiple objectives, including improving soil structure, promoting crop growth, and enhancing crop resistance.

[0005] Furthermore, the preparation process of liquid organic fertilizers also has some shortcomings. For example, in the preparation of liquid compound fertilizer in patent CN106365914B, the strain has a single function and fails to achieve multi-strain synergistic metabolism, resulting in limited fertilizer efficacy; the preparation efficiency and quality of the organic matrix need to be improved, affecting the fertilizer's nutrient supply and soil improvement effect; the activity of the microorganisms is easily damaged during the preparation process, and the fertilizer has poor stability, easily leading to precipitation and other problems, affecting the storage and use of the product.

[0006] Therefore, developing a microbial composite remediation liquid organic fertilizer that can simultaneously solve multiple problems such as heavy metal passivation, pesticide degradation, soil improvement, and crop growth promotion and stress resistance, and has advanced preparation technology and stable performance, is of great practical significance. Summary of the Invention

[0007] This invention provides a microbial compound remediation liquid organic fertilizer, comprising the following steps: (1) Inoculate Bacillus belye, Bacillus methylotrophicus and Pseudomonas putida in a 50L fermenter. Use 20g / L glucose as carbon source and 20g / L corn steep liquor as nitrogen source. Supplement with 1g / L ammonium sulfate, 1g / L K2HPO4 and 10mL / L trace element solution (containing FeSO4·7H2O, MnSO4·H2O, EDTA and other components). Maintain the initial pH at 7.0, temperature at 37℃, aeration rate at 1.5vvm and stirring speed at 200 rpm for 24-48 hours, and control glucose residue <2g / L. Methanol was added in batches at a rate of 5 g / L, and methanogenic archaea seed culture of 5%-15% v / v was inoculated simultaneously. Methane gas was continuously introduced at a flow rate of 0.1 vvm. Sodium acetate of 10 g / L was added as the main carbon source for methanogens, along with 5 mL / L sodium acetate, 5 g / L methanol, and 5 mL / L vitamin solution (containing B1, B2, and B6). 12 Add nicotinic acid and 0.5 g / L cysteine ​​hydrochloride, and continue fermentation for 48-72 hours until the effective viable count is ≥10. 9 CFU / mL. The fermentation broth mass ratio is (2-5):(2-5):(1-2):(0.1-1), which yields a highly active compound microbial agent.

[0008] (2) Crush the straw to a particle size of 2-5 mm, inoculate it with 1% Bacillus subtilis and mix it with the mushroom residue (mushroom cultivation waste) that has undergone solid-state fermentation with Aspergillus niger for 48 hours at a mass ratio, and add 2% urea to adjust the C / N ratio. Carry out aerobic composting fermentation in a fermentation tank, control the initial moisture content at 50-70%, turn the pile mechanically once every 24 hours, monitor the fermentation temperature throughout the process and maintain it at 55-60℃. After high-temperature fermentation for 7-15 minutes, stop fermentation when the pile temperature naturally drops below 40℃. Mix the fermentation product with water at a mass ratio, add cellulase and hemicellulase, and extract at a constant temperature of 45℃ with shaking for 24-48 hours. After centrifugation at 8000 rpm for 15 minutes, take the supernatant and filter it through a 0.45 μm microporous membrane to obtain agricultural waste fermentation liquid rich in humic products.

[0009] (3) The compound microbial agent and agricultural waste fermentation liquid were mixed in volume ratio, and functional additives were added in sequence: potassium humate, 0.1% seaweed extract, sophorolipid, 0.1% ascorbyl palmitate, and polyacrylamide. 0.2% xanthan gum and 0.05% sodium carboxymethyl starch were added to form a dual suspension system. After adjusting the pH of the system to 7.0 with 2M NaOH / HCl, microfiltration and ultrafiltration membrane separation were performed in sequence to finally obtain a high-concentration microbial compound remediation liquid organic fertilizer.

[0010] Furthermore, in step (1), the highly active compound microbial agent includes Bacillus belye, Pseudomonas putida, methyltrophic Bacillus, and methanogenic archaea.

[0011] The Bacillus belyssus has the preservation number CGMCC NO.21077 and is deposited at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0012] The *Pseudomonas putida* ZTB-A29 is classified and named *Pseudomonas putida*, with the accession number CGMCC NO.1.860, and is deposited at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0013] The methyltrophic Bacillus S3-1 has the preservation number CGMCC NO.1.10912 and is deposited at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0014] The methanogenic archaea has the preservation number CGMCC NO.1.12097 and is deposited at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0015] Furthermore, the fermentation broth mass ratio of Bacillus vesicularis, Pseudomonas putida, Bacillus methyltrophicus, and methanogenic archaea in the compound microbial agent is (2-5):(1-2):(2-5):(0.1-1). Furthermore, in step (2), the mass ratio of the crushed straw to the mushroom residue is 3-5:1; Furthermore, in step (2), 2% urea is added to adjust the C / N ratio to 20-25:1; Furthermore, in step (2), the mass ratio of the fermentation product to water is 1:3-7; Furthermore, in step (2), the amount of cellulase and hemicellulase added is 5-15 g / L and 10-20 g / L, respectively; Furthermore, in step (3), the mass ratio of the compound microbial agent to the agricultural waste fermentation liquid is 1:8-10; Furthermore, in step (3), the amounts of potassium humate, sophorolipid, and polyacrylamide added are 0.1%-0.5%, 0.3%-0.8%, and 0.1%-0.5%, respectively.

[0016] Furthermore, in step (3), the purpose of 0.22 μm microfiltration is to remove large-sized substances such as cell fragments and unused solid particles to obtain a clear fermentation broth. The purpose of using 10 kDa ultrafiltration is to retain large molecular weight humic acid with a molecular weight >10 kDa, while the permeate is composed of small molecular substances.

[0017] The beneficial effects of this invention are as follows: 1. Multi-dimensional ecological restoration efficiency Bacillus belyssus exhibits excellent stress resistance and biocontrol capabilities, effectively promoting plant root development, inhibiting pathogen growth, and enhancing nutrient absorption. Pseudomonas putida possesses strong metabolic diversity, effectively decomposing organic matter, improving soil fertility, enhancing root absorption of water and nutrients, and degrading harmful substances to purify the soil environment. Methyltrophic Bacillus utilizes methane and methanol as carbon sources, improving plant nutrient utilization, soil structure, and promoting plant growth. Methanogenic archaea are anaerobic microorganisms that can degrade organic matter and produce methane under anaerobic conditions, thus synergistically improving soil quality and promoting plant growth with other strains. Through the construction of a four-effect synergistic strain system, the dual effects of chemical passivation and biosorption of heavy metal pollutants, the enzymatic hydrolysis-mineralization synergistic degradation of recalcitrant pesticides, and the continuous supply of plant growth-promoting metabolites are achieved. This technology overcomes the limitations of traditional single-function remediation agents, significantly improving the comprehensive treatment efficiency of complex farmland pollution scenarios, and can achieve functional expansion through microbial carbon fixation metabolic pathways.

[0018] 2. Full-process process optimization system A dynamic, staged fermentation control strategy is employed to achieve high-density co-cultivation of functional strains and precise synthesis of target metabolites. A combined enzymatic hydrolysis-membrane separation coupling process fully releases functional organic matter components from agricultural waste. This process chain integrates multiple technologies to ensure the functional stability of the final product and its reliability in field applications.

[0019] 3. Green and sustainable technology pathways Using agricultural waste as a substrate, a resource recycling system is constructed. Through bioconversion, low-value materials such as straw and mushroom residue are transformed into high-value-added remediation materials, reducing raw material costs compared to traditional processes. The selected functional additives are all of natural origin or biodegradable materials, avoiding the environmental residue risks of chemically synthesized substances, and fully meeting the goals of agricultural carbon neutrality and the needs of circular economy development. Detailed Implementation

[0020] The following embodiments further illustrate the content of the present invention, but should not be construed as limiting the present invention. Any modifications or substitutions made to the methods, steps, or conditions of the present invention without departing from the spirit and essence of the invention are within the scope of the present invention.

[0021] Examples 1-5 1. The formulations of the microbial compound remediation liquid organic fertilizers in Examples 1-5 are shown in the table below: Table 1. Formulations of microbial compound remediation liquid organic fertilizers in Examples 1-5 2. The preparation steps of the microbial compound remediation liquid organic fertilizer in Examples 1-5 are as follows: (1) Inoculate 5% v / v Bacillus belye, 10% v / v methyltrophic Bacillus and 5% v / v Pseudomonas putida in a 50L fermenter. Use 20g / L glucose as carbon source and 20g / L corn steep liquor as nitrogen source. Supplement with 1g / L ammonium sulfate, 1g / L K2HPO4 and 10mL / L trace element solution (containing FeSO4·7H2O, MnSO4·H2O, EDTA and other components). Maintain the initial pH at 7.0, temperature at 37℃, aeration rate at 1.5vvm and stirring speed at 200 rpm for 8 hours. Control the glucose residue to <2g / L. Methanol was added in batches at a rate of 5 g / L, and 10% v / v methanogenic archaea seed culture was inoculated simultaneously. Methane gas was continuously introduced at a flow rate of 0.1 vvm. Sodium acetate was added at a rate of 10 g / L as the main carbon source for methanogens, along with 5 mL / L sodium acetate, 5 g / L methanol, and 5 mL / L vitamin solution (containing B1, B2, and B6). 12 Add nicotinic acid and 0.5 g / L cysteine ​​hydrochloride, and continue fermentation for 72 hours until the effective viable count is ≥10. 9 CFU / mL.

[0022] (2) Crush corn stalks to a particle size of 2-5 mm and sieve to remove impurities. Inoculate 1% Bacillus subtilis onto mushroom cultivation waste and ferment with Aspergillus niger in a solid state for 48 hours to degrade residual polysaccharides and lignin. Mix straw and mushroom residue at a mass ratio of 3:1, and add 2% urea to adjust the C / N ratio to 25:1. Carry out aerobic composting fermentation in a fermentation tank, with the initial moisture content controlled at 70%. Mechanically turn the pile once every 24 hours, and monitor the fermentation temperature throughout the process and maintain it at 60℃. After 10 days of fermentation, terminate the fermentation when the pile temperature naturally drops below 40℃. Mix the fermentation product with water at a mass ratio of 1:5, add 10 g / L cellulase and 15 g / L hemicellulase, and extract at a constant temperature of 45℃ with shaking for 48 hours. After centrifugation at 8000 rpm for 15 min, the supernatant was filtered through a 0.45 μm microporous membrane to obtain an agricultural waste fermentation broth rich in humic products.

[0023] (3) The compound microbial agent and agricultural waste fermentation liquid were mixed at a volume ratio of 1:9. Functional additives were added sequentially according to the formula: potassium humate, seaweed extract, sophorolipid, ascorbyl palmitate, and polyacrylamide. Xanthan gum and sodium carboxymethyl starch were added to form a double suspension system. The pH of the system was adjusted to 7.0 with 2M NaOH / HCl. The mixture was then passed through a 0.22 μm microfiltration membrane and a 10 kDa ultrafiltration membrane. The final product was a dark brown homogeneous liquid.

[0024] Example 6 The inoculum size of methylotrophic Bacillus was 5% v / v, and that of methanogenic archaea was 15% v / v. All other preparation conditions and methods were identical to those in Example 5.

[0025] Example 7 The inoculum size of methylotrophic Bacillus was 15% v / v, and that of methanogenic archaea was 5% v / v. All other preparation conditions and methods were identical to those in Example 5.

[0026] Comparative Examples 1-4 Four comparative examples were set up. The components of comparative examples 1-4 are shown in Table 2. Compared with the microbial compound remediation liquid organic fertilizer components of Example 5, comparative example 1 had no compound microbial agent, comparative example 2 had no mineral potassium humate, comparative example 3 had an excess of sophorolipid, and comparative example 4 had an insufficient amount of polyacrylamide.

[0027] Table 2. Formulations of microbial compound remediation liquid organic fertilizers for Comparative Examples 1-4 Monitoring of actual viable bacterial count in different embodiments Take 1g of each of the above Examples 1-7, dilute them in equal amounts, spread them on a plate, and test the number of viable bacteria. Repeat this process three times.

[0028] Table 3 Actual viable cell counts in Examples 1-7 Example 5 showed the best viable count at 10.5 × 10⁻⁶. 8 The CFU / mL concentration was significantly higher than in other examples (p<0.05), with an activity retention rate of 105%, indicating optimal synergistic effect of the strain and nutrient ratio. In Examples 1-4, within the range of 8%-14.85% of the bacterial agent ratio, the viable cell count showed a non-linear relationship with the bacterial agent ratio. Example 5 (10% bacterial agent) achieved optimal growth by balancing cell density and nutrient supply. In Example 6 (5% v / v of methyltrophic Bacillus), the viable cell count decreased to 9.2 × 10⁻⁶. 10 CFU / mL, possibly due to insufficient methanol induction leading to limited synthesis of growth-promoting metabolites. Example 7 (methanogenic archaea 5% v / v) viable count 8.8 × 10⁻⁶ 8 The CFU / mL count suggests that the methane metabolism pathway is blocked, affecting the energy supply of the bacterial community. In Example 5, the dual suspension system (xanthan gum + sodium carboxymethyl starch) and the antioxidant (ascorbate palmitate) jointly maintained bacterial activity, and the viable count remained ≥9.0 × 10⁻⁶ after 6 months of storage at room temperature. 8 CFU / mL.

[0029] Soil organic matter content enhancement test The experiment was conducted in the same experimental area, which was divided into several experimental blocks of the same area. After the soil organic matter content was tested and recorded, the fertilizers of the example and the comparison were applied respectively, with all parameters being the same. The time was 3 months. The soils after using the fertilizers of the example and the comparison were tested in accordance with GB9834-1988 "Soil Organic Matter Determination Method". The test results are shown in Table 4.

[0030] Table 4 Results of soil organic matter content enhancement test The results showed that Examples 4 and 5 performed best in improving soil organic matter content, which may be related to their optimized formulation ratios, particularly the synergistic effect of the compound microbial agent and functional additives. Examples 1, 2, 6, and 7 also showed some improvement, but were less effective than Examples 4 and 5, possibly due to differences in formulation ratios. Comparative Example 1 (without compound microbial agent) showed the lowest improvement in soil organic matter content, indicating that the compound microbial agent played a crucial role in soil improvement. Comparative Example 2 (without mineral-derived potassium humate) also showed a lower improvement value than the average of the examples, indicating that mineral-derived potassium humate also contributed to soil improvement. Comparative Examples 3 (excess sophorolipid) and 4 (insufficient polyacrylamide) also showed lower improvement values ​​than the examples, indicating that the proportion of functional additives needs to be precisely controlled to achieve the best soil improvement effect. These data indicate that by optimizing the formulation of microbial compound remediation liquid organic fertilizer, soil organic matter content can be significantly increased, thereby improving soil quality.

[0031] Heavy metal passivation capability test Artificially prepared Cd-containing 2 ⁺ (5 mg / kg), Pb 2 Simulated contaminated soil was treated with 500 mg / kg of fertilizer. Examples 1-5 and Comparative Examples 1-4 were set up, with a fertilizer application rate of 50 mL / kg. Samples were taken on days 7, 14, and 28, and the available heavy metal content was determined using the DTPA extraction method. Changes in soil pH and Eh values ​​were measured simultaneously. The reduction rate of available heavy metals was calculated as (available content before treatment - available content after treatment) / available content before treatment × 100%. The test results are shown in Table 5.

[0032] Table 5. Results of heavy metal passivation test The results showed that in Example 5, Pb 2 The best performance in terms of effective state reduction rate suggests that its formulation may be more focused on Pb. 2 Cd passivation. Other embodiments also showed some heavy metal passivation ability, but were inferior to those of Examples 4 and 5. Comparative Example 1 (without composite microbial agent) Cd 2 ⁺ The effective state reduction rate is low, and Pb 2 The effective state reduction rate was 0%, which further confirms the crucial role of the compound microbial agent in heavy metal passivation. Comparative Example 2 (potassium humate without mineral source) showed a Cd reduction rate of 0%. 2 - The effective state reduction rate is lower than the average level of the examples, but Pb 2 The high rate of reduction in bioavailable form suggests that potassium humate from mineral sources may have a significant impact on Cd. 2The passivation of ⁺ made a significant contribution. The heavy metal passivation capabilities of Comparative Example 3 (excess sophorolipid) and Comparative Example 4 (insufficient polyacrylamide) were also lower than those of the examples, indicating that the proportion of functional additives needs to be precisely controlled to achieve the best results.

[0033] Field planting effect After applying this fertilizer to farmland contaminated with heavy metals and pesticides, rice yield increased significantly by 15-25% compared to the control group, grain protein content increased by 5-10%, and the available cadmium content in the soil decreased by more than 65%. Among them, the formulation in Example 5 performed best, with an input-output ratio of 1:4.2, which was significantly better than conventional fertilizers. The verification of missing core components showed that the compound microbial agent is the key to promoting regeneration and remediation, and the mineral-derived potassium humate is crucial for soil improvement.

[0034] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A microbial compound remediation liquid organic fertilizer, characterized in that, Includes the following components by weight percentage: (1) Compound microbial inoculant 8%-15%; (2) Agricultural waste fermentation liquid: 84.45%-91.55%; (3) Functional additives: potassium humate (mineral source) 0.1%-0.5%; sophorolipid 0.3%-0.8%; polyacrylamide 0.1%-0.5%; seaweed extract 0.1%; ascorbyl palmitate 0.1%; xanthan gum 0.2%; sodium carboxymethyl starch 0.05%; The compound microbial agent is composed of Bacillus velezensis (CGMCC NO.21077), Pseudomonas putida ZTB-A29 (CGMCC NO.1.860), Bacillus methylotrophicus S3-1 (CGMCC NO.1.10912), and Methanosarcinasp. (CGMCC NO.1.12097), with a fermentation broth mass ratio of (2-5):(1-2):(2-5):(0.1-1). The agricultural waste fermentation liquid is prepared by mixing straw and Aspergillus niger solid fermentation residue at a mass ratio of 3-5:1, followed by aerobic composting fermentation and enzymatic hydrolysis by cellulase and hemicellulase.

2. The microbial composite remediation liquid organic fertilizer according to claim 1, characterized in that, The preparation method of the compound microbial agent includes the following steps: Crush straw to a particle size of 2-5 mm, inoculate with 1% Bacillus subtilis and mix with the residue fermented by Aspergillus niger for 48 hours at a mass ratio of (3-5):1, add 2% urea to adjust the C / N ratio to 20-25:1; carry out aerobic composting fermentation in a fermentation tank with an initial moisture content of 50-70%, mechanically turn the pile once every 24 hours, and maintain the fermentation temperature at 55-60℃ throughout the process; after 7-15 days of high-temperature fermentation, stop fermentation when the pile temperature naturally drops below 40℃; mix the fermentation product with water at a mass ratio of 1:(3-7), add 5-15 g / L cellulase and 10-20 g / L hemicellulase, and extract at a constant temperature of 45℃ for 24-48 hours with shaking; after centrifugation at 8000 rpm for 15 min, take the supernatant and filter it through a 0.45 μm microporous membrane to obtain agricultural waste fermentation liquid rich in humic products.

3. The microbial composite remediation liquid organic fertilizer according to claim 1, characterized in that, The preparation method of the agricultural waste fermentation liquid includes the following steps: (1) Crush the straw to a particle size of 2-5 mm, mix it with the residue of the fungus that has been fermented by Aspergillus niger in solid state for 48 hours at a mass ratio of 3-5:1, and add 2% urea to adjust the C / N ratio to 20-25:1; (2) Aerobic composting is carried out in the fermentation tank with an initial moisture content of 50-70%. The compost is turned mechanically every 24 hours to maintain a fermentation temperature of 55-60℃. High-temperature fermentation is terminated after 7-15 days. (3) Mix the fermentation product with water at a mass ratio of 1:3-7, add cellulase and hemicellulase, and extract at a constant temperature of 45°C for 24-48 hours by shaking. The product is then obtained by centrifugation and filtration through a microporous membrane.

4. The microbial composite remediation liquid organic fertilizer according to claim 1, characterized in that, The functional additives contain 0.2%-0.5% potassium humate, 0.5%-0.8% sophorolipid, and 0.2%-0.5% polyacrylamide.

5. The microbial compound remediation liquid organic fertilizer according to claim 1, characterized in that, The mass ratio of the compound microbial agent to the agricultural waste fermentation liquid is 1:8-10. After mixing, the pH is adjusted to 7.0 by 2M NaOH / HCl, and then subjected to 0.22μm microfiltration and 10kDa ultrafiltration separation treatment in sequence.

6. The microbial compound remediation liquid organic fertilizer according to any one of claims 1-5, characterized in that, The application of the fertilizer in the remediation of heavy metal contaminated soil is specifically manifested in its effect on Cd. 2 - The effective state decreases, affecting Pb 2 ⁺ The effective state decreases.

7. The microbial compound remediation liquid organic fertilizer according to any one of claims 1-5, characterized in that, The application of the fertilizer in pesticide residue degradation is specifically manifested in its high degradation rate of organophosphorus pesticides such as dichlorvos.

8. The microbial compound remediation liquid organic fertilizer according to any one of claims 1-5, characterized in that, The application of the fertilizer in soil quality improvement is specifically manifested in increased soil organic matter content and increased crop yield.

9. The microbial compound remediation liquid organic fertilizer according to any one of claims 1-5, characterized in that, The fertilizer has a room temperature storage stability of ≥6 months and a viable bacteria retention rate of ≥90%.

10. The microbial compound remediation liquid organic fertilizer according to any one of claims 1-5, characterized in that, The fertilizer process is optimized across the entire process through the following steps: (1) Dynamic staged fermentation control: High-density co-culture of four strains is achieved through a fed-batch fermentation strategy; (2) Composite enzymatic hydrolysis-membrane separation coupling process: functional organic matter components in agricultural waste are released through the synergistic action of cellulase and hemicellulase; (3) Construction of dual suspension system: The synergistic effect of xanthan gum and sodium carboxymethyl starch is used to maintain cell activity and product uniformity.

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