Compound enzyme, additive, application thereof and method for removal of fungal toxins

A technology of mycotoxins and compound enzymes, which is applied in the field of removing mycotoxins and compound enzymes, can solve the problems of less research, lower removal efficiency, and difficulty in removing multiple toxins

Active Publication Date: 2018-07-06
COFCO NUTRITION & HEALTH RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, in terms of biological removal, it is difficult to remove multiple toxins efficiently at the same time. When using a compound enzyme agent, the removal efficiency of one toxin is often improved, while the removal efficiency of other toxins is lower. Decreased
When

Method used

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  • Compound enzyme, additive, application thereof and method for removal of fungal toxins
  • Compound enzyme, additive, application thereof and method for removal of fungal toxins
  • Compound enzyme, additive, application thereof and method for removal of fungal toxins

Examples

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preparation example Construction

[0031] The present invention also provides a preparation method of the above-mentioned enzyme compound preparation, the method comprising: uniformly mixing each component according to the aforementioned weight ratio.

[0032] According to the present invention, the fumonisins may include their respective toxin types, for example, FA1, FA2, FB1, FB2, FB3, FB4, FC1, FC2, FC3, FC4 and FP1, among which fumonisin FB1 is most preferred. The ochratoxins may also include their respective toxin types, for example, ochratoxin A and ochratoxin B.

[0033] In the second aspect, the present invention also discloses an additive, wherein the additive contains the complex enzyme as described above.

[0034] In a preferred situation, the additive uses the complex enzyme provided by the invention as an active ingredient. In the additive, the content of the complex enzyme is 0.001-10 g / kg, preferably 0.01-8 g / kg, more preferably 0.1-5 g / kg. In addition, the additives may also contain solvents ...

preparation example 1

[0056] This preparation example is used to illustrate the preparation of amidase provided by the invention

[0057] (1) Acquisition of genes

[0058] The following nucleotide fragments were synthesized by artificial chemical synthesis (commissioned Bao Biological Engineering (Dalian) Co., Ltd., the same below); after the initiation codon ATG at the 5' end of the nucleotide sequence shown in SEQ ID NO:1 Add an Nde I restriction site, add an Xho I restriction site and a stop codon TAG at the 3' end.

[0059] (2) Construction of recombinant plasmids

[0060] Use restriction endonucleases Nde I and Xho I (purchased from NEB Company) to perform double enzyme digestion on the PET30a plasmid (with His tag, purchased from Invitrogen Company, USA), and digest it in a water bath at 37°C for 4 h, and the enzyme digestion system (50 μL) as follows:

[0061]

[0062] After agarose gel electrophoresis, the digested product was purified and recovered. Then, a ligation reaction was per...

preparation example 2-4

[0071] Preparation example 2-4 is used to illustrate the preparation of amidase provided by the invention

[0072] According to the method of Preparation Example 1, amidases X2-X4 were prepared respectively, the difference is that Preparation Examples 2-4 respectively used SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5 to replace SEQ ID NO: 1 .

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Abstract

Relating to the field of removal of fungal toxins, the invention discloses a compound enzyme, an additive, application thereof and a method for removal of fungal toxins. Specifically, the invention relates to a compound enzyme, which contains amidase and esterase and can remove fungal toxins, especially fumonisins, ochratoxins and T2 toxin, additives containing the compound enzyme and applicationthereof in removal of fungal toxins, especially fumonisins, ochratoxins and T2 toxin, and a method for removal of fungal toxins. According to the technical scheme, combined use of the amidase and esterase can achieve simultaneous removal of ochratoxin A, fumonisins and T2 toxin, the removal efficiency is greatly improved than single use of one enzyme, and vomitoxins, aflatoxins and zearalenone toxin can be removed to certain degree.

Description

technical field [0001] The present invention relates to the field of mycotoxin removal, in particular to a composite enzyme, an additive containing the composite enzyme, the application of the composite enzyme and / or additive in removing mycotoxins, and a method for removing mycotoxins Methods. Background technique [0002] Mycotoxins are a class of secondary metabolites produced by toxin-producing fungi during the damage process, mainly including aflatoxin, deoxynivalenol (also known as vomitoxin, DON), zearalenone (ZEN) and Fumonisins (FUM), ochratoxins and T-2 toxins, etc. [0003] In recent years, due to the frequent occurrence of extreme climates in my country, the head blight that mainly harms wheat and corn has become a pandemic, and the pollution of fumonisins (FUM), ochratoxin and T-2 produced by fungi has increased. Excessive wheat and corn are on the rise. Research on the safe use of grains with excessive toxins can not only protect the interests of farmers in o...

Claims

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Application Information

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IPC IPC(8): C12N9/78C12N9/16A23L5/20A23K20/189
CPCC12N9/16C12N9/78
Inventor 苏会波林海龙熊强唐堂谭剑黄锦张子剑李文钊李凡陈博臧传刚王靖朱镜羲
Owner COFCO NUTRITION & HEALTH RES INST
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