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Food additive comprising an amidase for detoxifying ochratoxin

A technology for food additives and ochratoxin, applied in food preparation, enzymes, enzymes, etc., can solve problems such as identification and display of proteins

Inactive Publication Date: 2013-07-17
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme activities other than carboxypeptidases have been reported for OTA degradation, but until now no one has been able to identify proteins showing OTA degradation activity

Method used

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  • Food additive comprising an amidase for detoxifying ochratoxin
  • Food additive comprising an amidase for detoxifying ochratoxin
  • Food additive comprising an amidase for detoxifying ochratoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1O

[0337] Example 1 OTA activity screening

[0338] Thirty enzymes including various lipases, proteases and amidases were investigated for their ability to degrade ochratoxin A (Table 1). Only carboxypeptidase A and lipase from Aspergillus niger (Amano TM A) Can decompose OTA, as previously reported (Pitout, 1969; Stander et al., 2000). In addition, the aspartic protease pepAd2 (Danisco) from Aspergillus niger showed OTA conversion activity, but this activity was inefficient. In order to facilitate the comparison of these three enzymes, the protein concentration of PEPAd was determined and the same concentration (1 mg / ml) of carboxypeptidase A and Amano TM Enzyme solution of lipase A. Perform degradation kinetics ( figure 1 ). figure 1 Show Amano TM The OTA degradation kinetics of lipase A and carboxypeptidase A were similar. After 7 hours of reaction at 37°C, no OTA peak was visible, implying complete conversion of OTA. For PEPAd, complete conversion was not achieved...

Embodiment 2

[0342] Example 2 Purification of ochratoxin from Aspergillus niger fermentation broth or filtered fermentation broth (cell-free) A degradation activity .

[0343] First, ammonium sulfate is used for precipitation. Several concentrations of ammonium sulfate were applied to the crude A. niger protein extract. OTA degradation activity was determined in supernatants and centrifuged pellets. By increasing the ammonium sulfate concentration, the OTA degradation activity was transferred from the supernatant to the centrifuged pellet. At 60% ammonium sulfate, all degradative activity exists in the centrifuge pellet. This shows that enzymes from 40% to 60% ammonium sulphate produced precipitates are capable of hydrolyzing mycotoxins. At the same salt concentration (60%), slight lipase activity remained in the centrifuged pellet (data not shown).

[0344] Fractions from 40% to 60% ammonium sulfate were used as starting material for further ochratoxin degrading enzyme purificatio...

Embodiment 3

[0357] Example 3 Improved Purification of Ochratoxin A Degrading Enzyme

[0358] Further improvements in purification by HIC and AEX could not be achieved by using different buffers and different gradient programs. Purification using phenyl sepharose can be considered as an affinity step because the OTA molecule has phenyl groups, however, impurities are highly hydrophobic and bind tightly to the phenyl sepharose medium and elute very late ( Figure 4 A). When using octyl sepharose and butyl sepharose, the OTA degradation active fraction was present in the unadsorbed flow-through fraction. This suggests that it has low hydrophobicity and that its tight association with phenyl sepharose is due to the affinity properties ( Figure 4 A), while the impurity is highly hydrophobic, it binds tightly and elutes later ( Figure 4 B), as in the case of phenyl-agarose.

[0359] The procedure was further refined using ammonium sulfate fractionation (AS), HIC / affinity chromatography ...

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Abstract

The present invention relates to amidase enzymes and a feed or food additive comprising the amidase enzyme capable of degrading ochratoxin.

Description

technical field [0001] The present invention relates to methods for detoxifying mycotoxins. More particularly, the present invention relates to amidases and feed and / or food additives comprising at least one amidase for detoxifying ochratoxins, especially ochratoxin A. Background technique [0002] Mycotoxins are toxic secondary products of fungi essentially belonging to the genera Aspergillus, Penicillium and Fusarium. They can be produced on a wide range of agricultural commodities and under various agronomic, ecological and post-harvest conditions worldwide. [0003] Mycotoxins can enter the food chain in the field, during storage of feed or food materials, or at later points in the food chain. Their accumulation in food and feed is a great threat to human and animal health, since consumption of mycotoxin-contaminated diets can lead to teratogenic, carcinogenic and estrogenic or immunosuppressive effects. [0004] In 1985, the World Health Organization estimated that a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/015A23L1/03A23L5/20A23L29/00
CPCA23K1/006A23L1/0153A23L1/034C12Y305/01004A23K1/1653C12N9/80A23K10/10A23K20/189A23L5/25A23L29/06
Inventor 于书坤C.H.波尔森S.达尔斯加尔德王华明I.尼科莱夫
Owner DUPONT NUTRITION BIOSCIENCES APS
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