Detoxifying pre-treated lignocellulose-containing materials

A lignocellulose and pretreatment technology, applied in the post-processing of cellulose pulp, fiber raw material processing, biofuels, etc., can solve problems such as expensive

Inactive Publication Date: 2009-12-23
NOVOZYMES NORTH AMERICA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This requires a large amount of water which needs to be removed again and is therefore expensive

Method used

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  • Detoxifying pre-treated lignocellulose-containing materials
  • Detoxifying pre-treated lignocellulose-containing materials
  • Detoxifying pre-treated lignocellulose-containing materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0332] Pretreatment of completely unwashed pretreated corn stover (fuwPCS)

[0333] Corn stover (PCS) erupted with dilute acid vapor was diluted with water and treated with NH 4 OH to pH 5.0. The total solids (TS) level was 15% by weight. This sample was then saccharified with Cellulolytic Preparation A at 50°C for 63 hours. Penicillin was added at a rate of 1 g / L and citrate buffer was also added at a rate of 50 mL of 1M citrate buffer per 100 ml of substrate prior to saccharification. Following the saccharification step, samples were filtered through a 0.2 micron Nalgene vacuum filter system (Product #8-0000-43-0803) to remove solids and used for fermentation. Then use a pipette to transfer the fuwPCS into a separate sterile container containing CO 2 15mL conical centrifuge tube with small vent.

[0334] Formulated (Gallic Acid)

[0335] with H 2 SO 4 The pH was adjusted to about 2; gallic acid was formulated at concentrations of 2 mM (GaA-L) and 10 mM (GaA-H). Ga...

Embodiment 2

[0345] Carbonic anhydrase and amidase

[0346] Pretreated corn stover saccharification

[0347] Corn stover (PCS) erupted with dilute acid vapor was diluted with water and treated with NH 4 OH to pH 5.0. The total solids (TS) level was 16%. This sample was then saccharified with Cellulolytic Preparation A at 50°C for 72 hours. Penicillin and citrate buffer were also added prior to saccharification. After the saccharification step, the samples were filtered to remove solids, and the filtrate was used for fermentation. Then use a pipette to transfer the fuwPCS into a separate sterile container containing CO 2 15mL conical centrifuge tube with small vent.

[0348] yeast preparation

[0349] Freeze-dried RED STAR TM Ethanol Red yeast was rehydrated in 10x YP medium for 30 minutes at 32°C. It was formulated into the fermentation at a dose of 0.2 g / L.

[0350] Preparation / detoxification

[0351] Detoxify filtered, unwashed PCS for 19 h using optimal conditions for ...

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Abstract

The invention relates to a process of detoxifying pre-treated lignocellulose-containing material by subjecting pre-treated material to a detoxifying compound capable of binding 1) pre-treated lignocellulose degradation products and / or 2) acetic acid. The detoxifying compound may also be an amidase and / or and anhydrase. The invention also relates to a process of producing a fermentation product including a detoxification process of the invention.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Application No. 60 / 870,420, filed December 18, 2006, and U.S. Provisional Application No. 60 / 890,652, filed February 20, 2007, under 35 U.S.C. 119, the contents of which are incorporated by reference in their entirety into this article. field of invention [0003] The present invention relates to methods of detoxifying pretreated lignocellulose-containing material. The present invention also relates to methods of producing fermentation products from lignocellulose-containing material using fermenting organisms comprising the detoxification methods of the present invention. Background of the invention [0004] Due to the limited reserves of fossil fuels and concerns about greenhouse gas emissions, there is increasing interest in the use of renewable energy sources. Production of fermentation products from lignocellulose-containing material is known in the art, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D21C9/00
CPCC12P7/10Y02E50/16D21C9/00Y02E50/10
Inventor 贾森·W·霍姆斯兰迪·戴因哈默宋子良
Owner NOVOZYMES NORTH AMERICA INC
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