Separation and purification method of trehalose

A technology for separation and purification of trehalose, applied in the field of sugar engineering, which can solve the problems of high cost of separation, complex process, inability to separate maltose and trehalose, and achieve the effects of environmental friendliness, low labor intensity and small production site

Active Publication Date: 2013-12-18
ZHUCHENG DONGXIAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Because maltose and trehalose are isomers, the above technical scheme cannot separate maltose and trehalose, and the above-mentioned process is complicated and the separation cost is expensive

Method used

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  • Separation and purification method of trehalose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The calcium-ion type polymer vinyl system gel type strong acid cation exchange resin described in embodiment 1 is purchased from Finex Oy company;

Embodiment 2

[0034] The calcium-ion type polymer vinyl system gel type strong acid cation exchange resin described in embodiment 2 is purchased from purolite company;

Embodiment 3

[0035] The calcium ion type polymer vinyl system gel type strong acid cation exchange resin described in Example 3 was purchased from Shandong Lukang Like Pharmaceutical Chemical Co., Ltd.;

[0036] The 001×7 cationic resin was purchased from Purolite Company; the 201×7 anionic resin was purchased from Purolite Company.

[0037] The trehalase reaction liquid in the examples was obtained by crushing and filtering the fermented liquid prepared by the method in Chinese patent document CN101831474A (application number 201010137964.2) with a high-pressure cell disruptor. The sugar components in the trehalase reaction liquid are maltose, glucose and trehalose.

[0038] Glucoamylase was purchased from Novozymes (China) Biotechnology Co., Ltd.

[0039] separation equipment

[0040]The simulated moving bed consists of five separation areas composed of 20 rotating chromatographic columns. Area Ⅰ is composed of chromatographic columns No. 1 to 3 connected in series, area II is composed...

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Abstract

The invention relates to a separation and purification method of trehalose, which comprises the following steps: (1) carrying out enzymolysis on a trehalase reaction solution prepared by a trehalose synthase conversion process, and filtering to obtain a trehalose crude solution; (2) decolorizing the trehalose crude solution through activated carbon, and filtering to obtain a mixed solution; (3) passing the mixed solution through an ion exchange resin to obtain a glucose-trehalose sugar solution; and (4) concentrating the glucose-trehalose sugar solution, and separating by continuous chromatography with a simulated moving bed to obtain a trehalose solution and a glucose solution. The trehalase reaction solution generated by converting maltose by trehalose synthase is used as the raw material, the maltose which is isomeric with the trehalose is converted by an enzyme process, and impurity glucose, remaining maltose and impurities, such as proteins, pigments, metallic ions and the like, are removed to obtain the high-purity trehalose.

Description

technical field [0001] The invention relates to a method for separating and purifying trehalose, in particular to a method for separating trehalose from mixed sugar liquid after biological fermentation by using special resin and simulated moving bed chromatography technology, and belongs to the technical field of sugar engineering. Background technique [0002] Trehalose is a non-reducing disaccharide formed by combining two glucose molecules through α, α-1,1 bonds. It widely exists in bacteria, fungi, algae, lower plants and insects. It has been found through research that this sugar has unique biological functions, such as protecting biological macromolecules, protecting cell membranes, and protecting proteins from damage caused by freezing, drying, and changes in osmotic pressure. It is widely used in food, medicine, cosmetics, agriculture, etc. application. [0003] At present, the demand for trehalose in the domestic and foreign markets continues to increase, and its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/04C07H1/06C12P19/14
Inventor 王腾飞王瑞明李丕武
Owner ZHUCHENG DONGXIAO BIOTECH
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