The application belongs to the technical field of
cell culture equipment, and provides a high-low
pressure cell culture device, which comprises a device box, a device cover movably installed on the device box, a high-pressure device cover and a low-pressure device cover, a plurality of
sieve-shaped partitions for placing
cell culture dishes and detachably arranged in the device box, and a first air inlet and
exhaust pipe installed on the sidewall of the device box, and further comprises a pressure regulating mechanism, which comprises a pressure regulating box, a driving
assembly, a first adjusting part, a fixing part, a second adjusting part and a second connecting part. The high-low
pressure cell culture device can control the
gas pressure in the device through the pressure regulating mechanism, completely separates the CO2
partial pressure control and
temperature control of the device from the device, controls the CO2
partial pressure and temperature in the device under different pressures by controlling the CO2 concentration and ambient temperature in the high-pressure gas source, and makes the device structure simpler and the operation more convenient.