Environment-friendly production method for enhancing trehalose yield of beer waste yeast under stress conditions

A technology for waste beer yeast and a production method, applied in the field of environmental protection production, can solve the problems of low extraction rate, difficult impurity dissolution, long extraction time of trehalose, etc., and achieve the effect of short processing time

Active Publication Date: 2014-12-03
HEZE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides an environment-friendly production method that uses stress conditions to increase the yield of trehalose from beer waste yeast, in order to sol

Method used

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  • Environment-friendly production method for enhancing trehalose yield of beer waste yeast under stress conditions

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Effect test

Embodiment 1

[0033] Such as figure 1 Shown, a kind of environment-friendly production method that utilizes stress condition to improve the trehalose yield of beer waste yeast comprises the following steps:

[0034] 1) Microwave treatment

[0035] First, debitterize and purify the waste beer yeast sludge. The debittering and purification of the waste beer yeast sludge includes the following steps: add pure water to the waste beer yeast sludge (the weight ratio of beer waste yeast sludge to pure water is 1:3), after 80 ~ 100 mesh sieve to obtain yeast suspension; add tartaric acid to the obtained yeast suspension, so that the mass fraction of tartaric acid is 5%, stir for 60 minutes to remove bitterness, and centrifuge to discard the supernatant; 300-mesh sieve, washed with water again, and centrifuged to obtain purified and debittered yeast sludge; then place the debittered and purified yeast sludge in a 500W microwave oven for 5 minutes;

[0036] 2) heat treatment

[0037] Add pure wate...

Embodiment 2

[0045] Such as figure 1 Shown, a kind of environment-friendly production method that utilizes stress condition to improve the trehalose yield of beer waste yeast comprises the following steps:

[0046] 1) Microwave treatment

[0047] First, debitterize and purify the waste beer yeast sludge. The debittering and purification of the waste beer yeast sludge includes the following steps: add pure water to the waste beer yeast sludge (the weight ratio of beer waste yeast sludge to pure water is 1:3), after 80 ~ 100 mesh sieve to obtain yeast suspension; add tartaric acid to the obtained yeast suspension, so that the mass fraction of tartaric acid is 5%, stir for 60 minutes to remove bitterness, and centrifuge to discard the supernatant; 300-mesh sieve, washed with water again, and centrifuged to obtain purified and debittered yeast sludge; then place the debittered and purified yeast sludge in a 500W microwave oven for 5 minutes;

[0048] 2) heat treatment

[0049] Add pure water ...

Embodiment 3

[0057] Such as figure 1 Shown, a kind of environment-friendly production method that utilizes stress condition to improve the trehalose yield of beer waste yeast comprises the following steps:

[0058] 1) Microwave treatment

[0059] First, debitterize and purify the waste beer yeast sludge. The debittering and purification of the waste beer yeast sludge includes the following steps: add pure water to the waste beer yeast sludge (the weight ratio of beer waste yeast sludge to pure water is 1:3), after 80 ~ 100 mesh sieve to obtain yeast suspension; add tartaric acid to the obtained yeast suspension, so that the mass fraction of tartaric acid is 8%, stir for 30min to remove bitterness, and centrifuge to discard the supernatant; 300-mesh sieve, washed with water again, and centrifuged to obtain purified and debittered yeast sludge; then place the debittered and purified yeast sludge in a 600W microwave oven for 1 min;

[0060] 2) heat treatment

[0061] Add pure water to the ...

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Abstract

The invention discloses an environment-friendly production method for enhancing trehalose yield of beer waste yeast under stress conditions, belonging to the field of biochemical engineering and aiming to solve the problems of long trehalose extraction time, low extraction rate and difficulty in purification due to too many impurities dissolved in the prior art. The method comprises the following steps: 1) microwave treatment; 2) heat treatment; 3) helicase treatment; 4) ultrasonic crushing treatment; and 5) trehalose extraction and purification. According to the method, the yeast is under stress conditions to generate abundant trehalose; meanwhile, the activity of the trehalase is inhibited, and the yeast is subjected to helicase treatment and ultrasonic crushing treatment to rupture the yeast cells, so that almost all the trehalose is dissolved out; and thus, the method has the advantages of short treatment time, easy trehalose purification and high yield. Besides, the method does not use any strong acid, strong alkali or toxic organic solvent, and implements cleanness and environmental protection; and the waste liquid and waste residue generated in the method can be used for producing yeast extract, so almost no waste liquid or waste residue is discharged.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to an environment-friendly production method for increasing the yield of trehalose from beer waste yeast by using stress conditions. Background technique [0002] Trehalose is a non-reducing disaccharide composed of two glucose molecules with a 1,1-glycosidic bond, and is widely distributed in bacteria, fungi, animals and plants. In organisms, it not only exists as a structural component and energy substance, but also has a specific protective effect on biological tissues and biological macromolecules under stress conditions such as high cold, high temperature, and dehydration. Due to its unique biological functions, it has broad application prospects in food, molecular biology, medicine, cosmetics, agriculture, etc. Although trehalose has a wide range of resources, there are not many species that can accumulate trehalose in large quantities, and trehalose is a metabolic stres...

Claims

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Application Information

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IPC IPC(8): C07H3/04C07H1/06
Inventor 王宜磊朱陶张海丽宋磊
Owner HEZE UNIV
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